| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97710-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99108-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06652-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98811-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90017-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09026-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12016-0.025mg | |||
![Western blot analysis on different lysates with NBP3-33050 at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HCT 116 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: HT-29 cell lysate Lane 8: HUVEC cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 40 kDa Observed band size: 42 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33050 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33050_-western-blot-639204595248910235.jpg "Western blot analysis on different lysates with NBP3-33050 at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HCT 116 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: HT-29 cell lysate Lane 8: HUVEC cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 40 kDa Observed band size: 42 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33050 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PARP10 Antibody [NBP2-88012] - WB Suggested Anti-PARP10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/PARP10-Antibody-Western-Blot-NBP2-88012-img0001.jpg "Western Blot: PARP10 Antibody [NBP2-88012] - WB Suggested Anti-PARP10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate")
![Immunohistochemistry-Paraffin: PARP4 Antibody [NBP2-97710] - Immunochemical staining of human PARP4 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PARP4-Antibody-Immunohistochemistry-Paraffin-NBP2-97710-img0001.jpg "Immunohistochemistry-Paraffin: PARP4 Antibody [NBP2-97710] - Immunochemical staining of human PARP4 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunoprecipitation: Nox4 Antibody [NBP2-99108] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-NOX4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NOX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions. Predicted band size: 64 kDaObserved band size: 71 kDa](http://images.novusbio.com/fullsize/Nox4-Antibody-Immunoprecipitation-NBP2-99108-img0001.jpg "Immunoprecipitation: Nox4 Antibody [NBP2-99108] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-NOX4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NOX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions. Predicted band size: 64 kDaObserved band size: 71 kDa")
![Flow Cytometry: UbcH5a/UBE2D1 Antibody (07) [NBP3-06652] - Flow cytometric analysis of Human UBE2D1 expression on A431 cells. Cells were stained with purified anti-Human UBE2D1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/UbcH5a-UBE2D1-Antibody-07-Flow-Cytometry-NBP3-06652-img0001.jpg "Flow Cytometry: UbcH5a/UBE2D1 Antibody (07) [NBP3-06652] - Flow cytometric analysis of Human UBE2D1 expression on A431 cells. Cells were stained with purified anti-Human UBE2D1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP2-98811] - Immunofluorescence staining of JNK2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with NBP2-98811 antibody (1:1000)(Figure A), incubated with NBP2-98811 antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98811_rabbit-polyclonal-jnk2-antibody-23520252135112.png "Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP2-98811] - Immunofluorescence staining of JNK2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with NBP2-98811 antibody (1:1000)(Figure A), incubated with NBP2-98811 antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.")
![Western blot analysis on different lysates with NBP3-32779 at 1/5,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 289 kDa Observed band size: 289 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32779 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32779_-western-blot-639204595240263937.jpg "Western blot analysis on different lysates with NBP3-32779 at 1/5,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 289 kDa Observed band size: 289 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32779 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Chromatin Immunoprecipitation: KAT3B/p300 Antibody (1H11G2) [NBP2-59233] - ChIP was performed using HeLa cells, the antibody against p300 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for two genomic regions near the ANKRD32 and IRS2 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and an intergeic region on chromosome 11, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-1H11G2-Chromatin-Immunoprecipitation-NBP2-59233-img0001.jpg "Chromatin Immunoprecipitation: KAT3B/p300 Antibody (1H11G2) [NBP2-59233] - ChIP was performed using HeLa cells, the antibody against p300 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for two genomic regions near the ANKRD32 and IRS2 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and an intergeic region on chromosome 11, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![Western Blot: TRIB3 Antibody (2D1) [H00057761-M08] - TRIB3 monoclonal antibody (M08), clone 2D1 Western Blot analysis of TRIB3 expression in HepG2 ( Cat # L019V1 ).](http://images.novusbio.com/fullsize/TRIB3-Antibody-2D1-Western-Blot-H00057761-M08-img0001.jpg "Western Blot: TRIB3 Antibody (2D1) [H00057761-M08] - TRIB3 monoclonal antibody (M08), clone 2D1 Western Blot analysis of TRIB3 expression in HepG2 ( Cat # L019V1 ).")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (IDEC-131 (Toralizumab)) - Chimeric [NBP3-12016] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of IDEC-131 (Toralizumab) (NBP3-12016) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12016, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-IDEC-131-Toralizumab-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12016-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (IDEC-131 (Toralizumab)) - Chimeric [NBP3-12016] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of IDEC-131 (Toralizumab) (NBP3-12016) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12016, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")