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![Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate](http://images.novusbio.com/fullsize/nbp3-26016_rabbit-histone-h4-mab-rm202-azide-and-bsa-free-20620241532491.png "Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate")
![Multiplex Immunoassay: Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - RM202 specifically reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with unmodified Lysine 12 (K126 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26016_rabbit-histone-h4-mab-rm202-azide-and-bsa-free-2462024133249.png)
![Western Blot: Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM202 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26016_rabbit-histone-h4-mab-rm202-azide-and-bsa-free-22620242311076.png)
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP3-26016] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM202 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26016_rabbit-histone-h4-mab-rm202-azide-and-bsa-free-22620242311034.png)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Western-Blot-NBP2-42860-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [282] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Immunocytochemistry-Immunofluorescence-NBP2-42860-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Chromatin-Immunoprecipitation-ChIP-NBP2-42860-img0001.jpg)
![Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of HIST1H4A protein from HeLa whole cell extracts using 5 ug of Histone H4K20me2 (dimethyl Lys20) antibody [282]. Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [282]. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Immunoprecipitation-NBP2-42860-img0004.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Western-Blot-NBP2-42861-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [1851] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Immunocytochemistry-Immunofluorescence-NBP2-42861-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Chromatin-Immunoprecipitation-NBP2-42861-img0001.jpg)
![Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of HIST1H4A protein from HeLa whole cell extracts using 5 ug of Histone H4K20me2 (dimethyl Lys20) antibody [1851]. Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Immunoprecipitation-NBP2-42861-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - Mouse NIH3T3 cells were stained with the antibody against H4K5,8,12,16ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K5,8,12,16ac antibody (left) diluted 1:500 in blocking solution followed by an anti- rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys13--ac-Lys5--ac-Lys8]-Antibody-Immunofluorescence-NBP2-59253-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - Mouse NIH3T3 cells were stained with the antibody against H4K5,8,12,16ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K5,8,12,16ac antibody (left) diluted 1:500 in blocking solution followed by an anti- rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - ChIP assays were performed using human HeLa cells, the antibody against H4K5,8,12,16ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP- seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys13--ac-Lys5--ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-59253-img0002.jpg)
![ELISA: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K5,8,12,16ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,200.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys13--ac-Lys5--ac-Lys8]-Antibody-ELISA-NBP2-59253-img0001.jpg)
![Dot Blot: Histone H4 [ac Lys13, ac Lys5, ac Lys8] Antibody [NBP2-59253] - One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Thr figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys13-ac-Lys5-ac-Lys8]-Antibody-Dot-Blot-NBP2-59253-img0004.jpg)
![Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-Western-Blot-NBP1-70006-img0004.jpg "Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.")
![Immunohistochemistry-Paraffin: HA Tag Antibody [NBP1-70006] - Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 um were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-Immunohistochemistry-Paraffin-NBP1-70006-img0001.jpg)
![Western Blot: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H4K20me1. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Western-Blot-NBP2-59258-img0001.jpg "Western Blot: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H4K20me1. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - HeLa cells were stained with the antibody against H4K20me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K20me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Immunofluorescence-NBP2-59258-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - ChIP assays were performed using human HeLa cells, the antibody against H4K20me1 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the GAPDH promoter and a region located 1 kb upstream of the GAPDH promoter, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NBP2-59258-img0003.jpg)
![ELISA: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H4K20me1 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:51,100.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-ELISA-NBP2-59258-img0002.jpg)
![Western Blot: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4R3me2(sym). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Arg3]-Antibody-Western-Blot-NBP2-59265-img0001.jpg "Western Blot: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4R3me2(sym). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - HeLa cells were stained with the antibody against H4R3me2(sym) and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4R3me2(sym) antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Arg3]-Antibody-Immunofluorescence-NBP2-59265-img0004.jpg)
![Dot Blot: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - To test the cross reactivity of the antibody against H4R3me2(sym), a Dot Blot analysis was performed with peptides containing other histone arginine methylations and the unmodified H4R3. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows the antibody is specific for the H4R3 symmetric dimethylation and also recognizes the H2AR3 symmetric dimethylation.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Arg3]-Antibody-Dot-Blot-NBP2-59265-img0002.jpg)
![ELISA: Histone H4 [Dimethyl Arg3] Antibody [NBP2-59265] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4R3me2(sym) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,750.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Arg3]-Antibody-ELISA-NBP2-59265-img0003.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K20me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Western-Blot-NBP2-59246-img0001.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K20me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - HeLa cells were stained with the antibody against H4K20me2 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H4K20me2 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Immunofluorescence-NBP2-59246-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K20me2 and optimized PCR primer sets for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit on the IP-Star automated system, using sheared chromatin from 1 million cells. Respectively 1 and 5 ug of antibody was used per ChIP experiment. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and SatA satellite repeats, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Chromatin-Immunoprecipitation-NBP2-59246-img0002.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - To test the cross reactivity of the antibody against H4K20me2, analysis was performed with peptides containing other histone modifications and the unmodified H4K20. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Dot-Blot-NBP2-59246-img0004.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody (PS01-25) [NBP3-32432] - Western blot analysis of Histone H4 on different lysates with Rabbit anti-Histone H4 antibody (NBP3-32432) at 1/500 dilution. Lane 1: Hela cell lysate, 10 ug/Lane Lane 2: NIH/3T3 cell lysate, 10 ug/Lane Lane 3: PC-12 cell lysate, 10 ug/Lane Predicted band size: 11 kDa Observed band size: 14/16 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32432) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32432_rabbit-histone-h4-ac-lys8-mab-ps01-25-278202416285290.jpg "Western Blot: Histone H4 [ac Lys8] Antibody (PS01-25) [NBP3-32432] - Western blot analysis of Histone H4 on different lysates with Rabbit anti-Histone H4 antibody (NBP3-32432) at 1/500 dilution. Lane 1: Hela cell lysate, 10 ug/Lane Lane 2: NIH/3T3 cell lysate, 10 ug/Lane Lane 3: PC-12 cell lysate, 10 ug/Lane Predicted band size: 11 kDa Observed band size: 14/16 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32432) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Histone H4 [ac Lys8] Antibody (PS01-25) [NBP3-32432] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H4 antibody (NBP3-32432) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32432) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32432_rabbit-histone-h4-ac-lys8-mab-ps01-25-298202414161864.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody (PS01-25) [NBP3-32432] - Immunocytochemistry analysis of PC-12 cells labeling Histone H4 with Rabbit anti-Histone H4 antibody (NBP3-32432) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H4 antibody (NBP3-32432) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32432_rabbit-histone-h4-ac-lys8-mab-ps01-25-2920241192363.jpg)
![Western Blot: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4, using Clone RM205 at 1 ug/mL, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26013_rabbit-histone-h4-mab-rm205-azide-and-bsa-free-226202422321211.png "Western Blot: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4, using Clone RM205 at 1 ug/mL, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] - RM205 specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26013_rabbit-histone-h4-mab-rm205-azide-and-bsa-free-2462024132646.png)
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM205 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26013_rabbit-histone-h4-mab-rm205-azide-and-bsa-free-226202422174414.png)
![Multiplex Immunoassay: Histone H4 [ac Lys8] Antibody (RM201) - Azide and BSA Free [NBP3-26011] - RM201 specifically reacts to Histone H4 acetylated at Lysine 8. No cross reactivity with unmodified Lysine 8, acetylated Lysine 5, Lysine 12, Lysine 16, Lysine 20, Lysine 31, or Lysine 91 in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26011_rabbit-histone-h4-mab-rm201-azide-and-bsa-free-2462024124598.png "Multiplex Immunoassay: Histone H4 [ac Lys8] Antibody (RM201) - Azide and BSA Free [NBP3-26011] - RM201 specifically reacts to Histone H4 acetylated at Lysine 8. No cross reactivity with unmodified Lysine 8, acetylated Lysine 5, Lysine 12, Lysine 16, Lysine 20, Lysine 31, or Lysine 91 in Histone H4.")
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody (RM201) - Azide and BSA Free [NBP3-26011] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM201 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26011_rabbit-histone-h4-mab-rm201-azide-and-bsa-free-22620242311031.png)
![Western Blot: Histone H4 [ac Lys8] Antibody (RM201) - Azide and BSA Free [NBP3-26011] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4, using RM201 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26011_rabbit-histone-h4-mab-rm201-azide-and-bsa-free-246202413266.png)
![Western Blot: Histone H4 [ac Lys8] Antibody (RM201) [NBP2-61558] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4. Using NBP2-61558, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-RM201-Western-Blot-NBP2-61558-img0009.jpg "Western Blot: Histone H4 [ac Lys8] Antibody (RM201) [NBP2-61558] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4. Using NBP2-61558, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody (RM201) [NBP2-61558] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61558 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-RM201-Immunocytochemistry-Immunofluorescence-NBP2-61558-img0008.jpg)
![Multiplex Immunoassay: Histone H4 [ac Lys8] Antibody (RM201) [NBP2-61558] - NBP2-61558 specifically reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with unmodified Lysine 8 (K8 ctrl), acetylated Lysine 5 (K5ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-RM201-Multiplex-Immunoassay-NBP2-61558-img0010.jpg)