| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26049 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16137-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26011 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26016 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12019-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26008 | |||
![Western Blot: HA Tag Antibody (RM305) [NBP2-61477] - 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61477 at 0.1 ug/ml](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RM305-Western-Blot-NBP2-61477-img0003.jpg "Western Blot: HA Tag Antibody (RM305) [NBP2-61477] - 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61477 at 0.1 ug/ml")
![Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) [NBP2-61477] - Staining of LNCaP cells overexpressing HA-tag Bag1 protein, using NBP2-61477 at 0.01 ug/ml](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RM305-Immunohistochemistry-Paraffin-NBP2-61477-img0001.jpg)
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from NIH/3T3 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511392278.jpg "Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from NIH/3T3 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from C6 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511374158.jpg)
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from MCF7 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511365166.jpg)
![Dot Blot: Histone H4 [Lactyl Lys5] Antibody [NBP3-16137] - Dot-blot analysis of all sorts of peptides using Histone H4 antibody (NBP3-16137) at 1:4000 dilution.](http://images.novusbio.com/fullsize/Histone-H4-[Lactyl-Lys5]-Antibody-Dot-Blot-NBP3-16137-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody (S05-8H9) [NBP3-20040] - Immunohistochemistry of paraffin-embedded Human colon cancer tissue using NBP3-20040 at dilution 1/100](http://images.novusbio.com/fullsize/nbp3-20040_rabbit-histone-h4-mab-s05-8h9-103202523464552.jpg "Immunohistochemistry-Paraffin: Histone H4 Antibody (S05-8H9) [NBP3-20040] - Immunohistochemistry of paraffin-embedded Human colon cancer tissue using NBP3-20040 at dilution 1/100")
![Western Blot: Histone H4 [ac Lys5] Antibody (RM199) - Azide and BSA Free [NBP3-26010] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, and (2) recombinant Histone H4, using RM199 at 0.2 ug/mL, showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26010_rabbit-histone-h4-mab-rm199-azide-and-bsa-free-24620241332443.png "Western Blot: Histone H4 [ac Lys5] Antibody (RM199) - Azide and BSA Free [NBP3-26010] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, and (2) recombinant Histone H4, using RM199 at 0.2 ug/mL, showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys5] Antibody (RM199) - Azide and BSA Free [NBP3-26010] - RM199 specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with unmodified Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26010_rabbit-histone-h4-mab-rm199-azide-and-bsa-free-22620242311039.png)
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys5] Antibody (RM199) - Azide and BSA Free [NBP3-26010] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM199 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26010_rabbit-histone-h4-mab-rm199-azide-and-bsa-free-226202422321222.png)
![Western Blot: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4, using RM194 at 0.5 ug/mL, showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26008_rabbit-histone-h4-mab-rm194-azide-and-bsa-free-246202413265.png "Western Blot: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4, using RM194 at 0.5 ug/mL, showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] - RM194 specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).](http://images.novusbio.com/fullsize/nbp3-26008_rabbit-histone-h4-mab-rm194-azide-and-bsa-free-24620241332410.png)
![Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] - Immunocytochemistry of HeLa cells, using Clone RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26008_rabbit-histone-h4-mab-rm194-azide-and-bsa-free-24620241332413.png)
![Western Blot: Histone H4 [ac Lys8] Antibody (RM201) [NBP2-61558] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4. Using NBP2-61558, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-RM201-Western-Blot-NBP2-61558-img0009.jpg "Western Blot: Histone H4 [ac Lys8] Antibody (RM201) [NBP2-61558] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4. Using NBP2-61558, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.")
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody (RM201) - Azide and BSA Free [NBP2-61558] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM201 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61558_rabbit-monoclonal-histone-h4-ac-lys8-antibody-rm201-immunocytochemistry-immunofluorescence-189202523403510.png)
![Western Blot: Histone H4 [ac Lys12] Antibody (RM202) [NBP2-61554] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61554, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-RM202-Western-Blot-NBP2-61554-img0006.jpg "Western Blot: Histone H4 [ac Lys12] Antibody (RM202) [NBP2-61554] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61554, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.")
![Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys12] Antibody (RM202) - Azide and BSA Free [NBP2-61554] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM202 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61554_rabbit-monoclonal-histone-h4-ac-lys12-antibody-rm202-immunocytochemistry-immunofluorescence-18920252339523.png)
![Western Blot: Histone H4 [ac Lys16] Antibody (C.15200219) [NBP2-59242] - Histone extracts (15 ug) from HeLa cells were analysed using the antibody against H4K16ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-C-15200219-Western-Blot-NBP2-59242-img0001.jpg "Western Blot: Histone H4 [ac Lys16] Antibody (C.15200219) [NBP2-59242] - Histone extracts (15 ug) from HeLa cells were analysed using the antibody against H4K16ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys16] Antibody (C.15200219) [NBP2-59242] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K16ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis)](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-C-15200219-Chromatin-Immunoprecipitation-NBP2-59242-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys16] Antibody (C.15200219) [NBP2-59242] - To test the cross reactivity of the antibody against H4K16ac, analysis was performed with peptides containing other acetylations and unmodified sequences of histone H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-C-15200219-Dot-Blot-NBP2-59242-img0004.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K8ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Western-Blot-NBP2-59240-img0001.jpg "Western Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K8ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Analysis was performed using HeLa cells, the monoclonal antibody against H4K8ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the EIF4A2 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Chromatin-Immunoprecipitation-NBP2-59240-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - To test the cross reactivity of the antibody against H4K8ac, analysis was performed with peptides containing other acetylations and unmodified sequences of histone H4. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Dot-Blot-NBP2-59240-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric [NBP3-12019] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of KM-2 (NBP3-12019) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12019, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-ac-Lys16-ac-Lys8-Antibody-KM-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12019-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric [NBP3-12019] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of KM-2 (NBP3-12019) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12019, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric - Azide and BSA Free [NBP3-12019] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12019 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12019_rabbit-monoclonal-histone-h4-ac-lys12-ac-lys16-ac-lys8-antibody-km-2-chimeric-74202522113023.png)
![Western Blot: Histone H4 [p Ser1] Antibody (RM194) [NBP2-61553] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using NBP2-61553, showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-p-Ser1-Antibody-RM194-Western-Blot-NBP2-61553-img0007.jpg "Western Blot: Histone H4 [p Ser1] Antibody (RM194) [NBP2-61553] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using NBP2-61553, showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.")
![Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP2-61553] - Immunocytochemistry of HeLa cells, using Clone RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61553_rabbit-monoclonal-histone-h4-p-ser1-antibody-rm194-immunocytochemistry-immunofluorescence-189202523403515.png)