| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52512-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05169-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-21805-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54540-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48417 | |||
| Azide and BSA Free | NBP3-11458 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79854 | |||
![Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NB100-1883-img0001.jpg "Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Western-Blot-NBP3-15638-img0005.jpg "Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunoprecipitation: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of 300ug extracts of 293T cells using 3ug GRB2 antibody (NBP3-15638). Western blot was performed from the immunoprecipitate using GRB2 (NBP3-15638) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunoprecipitation-NBP3-15638-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (9K6H0) [GRB2] - Confocal imaging of HeLa cells using GRB2 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15638_rabbit-grb2-mab-9k6h0-52202517344210.jpg)
![Immunohistochemistry: GRB2 Antibody (9K6H0) [GRB2] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using GRB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15638_rabbit-grb2-mab-9k6h0-52202517333886.jpg)
![Immunohistochemistry: GRB2 Antibody (9K6H0) [GRB2] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GRB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15638_rabbit-grb2-mab-9k6h0-5220251731554.jpg)
![Immunohistochemistry: GRB2 Antibody (9K6H0) [GRB2] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using GRB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15638_rabbit-grb2-mab-9k6h0-52202517352718.jpg)
![Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Western-Blot-NBP2-52512-img0004.jpg "Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)")
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of bladder cancer tissues using MMP14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Immunohistochemistry-Paraffin-NBP2-52512-img0003.jpg)
![Flow Cytometry: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of HT1080 cells using MMP14 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Flow-Cytometry-NBP2-52512-img0001.jpg)
![Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against HEK293 (1) and MMP14 (AA: 112-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Western-Blot-NBP2-52512-img0005.jpg)
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of ovarian cancer tissues using MMP14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Immunohistochemistry-NBP2-52512-img0002.jpg)
![ELISA: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-ELISA-NBP2-52512-img0006.jpg)
![Immunohistochemistry-Paraffin: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with NBP1-05169 developed with peroxidase-labeled goat anti-mouse Ig (blue color).](http://images.novusbio.com/fullsize/Procollagen-Type-2A-Antibody-003-02-Immunohistochemistry-Paraffin-NBP1-05169-img0005.jpg "Immunohistochemistry-Paraffin: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with NBP1-05169 developed with peroxidase-labeled goat anti-mouse Ig (blue color).")
![Immunohistochemistry: Collagen II Antibody (003-02) - BSA Free [NBP1-05169] - Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Positive stain of Canine Chondrocytes incubated overnight at room temperature at 1:100 dilution. Image courtesy of Dragana Ponjevic, Dr. Matyas Laboratory, University of Calgary.](http://images.novusbio.com/fullsize/Procollagen-Type-2A-Antibody-003-02-Immunohistochemistry-NBP1-05169-img0003.jpg)
![Immunohistochemistry: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Human chondrocytes stained with NBP1-05169 overnight at room temperature, 1:100 dilution. Image courtesy of Dragana Ponjevic, Dr. Matyas Laboratory, University of Calgary.](http://images.novusbio.com/fullsize/Procollagen-Type-2A-Antibody-003-02-Immunohistochemistry-NBP1-05169-img0004.jpg)
![Immunohistochemistry-Paraffin: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Staining of a formalin-fixed, paraffin-embedded fetal bone anlage in the hand (around week 10).](http://images.novusbio.com/fullsize/Procollagen-Type-2A-Antibody-003-02-Immunohistochemistry-Paraffin-NBP1-05169-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunofluorescence-NBP2-21805-img0002.jpg "Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Immunohistochemistry: N-Cadherin Antibody (GC-4) [NBP2-21805] - Frozen acetone fixed chicken cardiac sections stained with Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunohistochemistry-NBP2-21805-img0001.jpg)
![Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Western blot analysis of MUC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Western-Blot-NBP2-67650-img0009.jpg "Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Western blot analysis of MUC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody (SN06-80) [NBP2-67650] - Staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunocytochemistry-Immunofluorescence-NBP2-67650-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0008.jpg)
![Flow Cytometry: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Flow-Cytometry-NBP2-67650-img0001.jpg)
![Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Western-Blot-NBP2-67650-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody (SN06-80) [NBP2-67650] - Staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunocytochemistry-Immunofluorescence-NBP2-67650-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0005.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0012.jpg "Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi")
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0011.jpg)
![Flow Cytometry: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Flow-Cytometry-NBP2-67327-img0001.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0002.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0003.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0004.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0005.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0008.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0009.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0010.jpg)
![Western Blot: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Breast cancer cell lysates were probed with Mouse anti-Human Beta-Catenin antibody (#NBP2-53366-20ug) at 0.5 ug/mL, followed by 1:5,000 anti-Mouse secondary antibody. Image is courtesy of customer review.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Western-Blot-NBP2-53366-img0005.jpg "Western Blot: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Breast cancer cell lysates were probed with Mouse anti-Human Beta-Catenin antibody (#NBP2-53366-20ug) at 0.5 ug/mL, followed by 1:5,000 anti-Mouse secondary antibody. Image is courtesy of customer review.")
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded Rat Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Immunohistochemistry-Paraffin-NBP2-53366-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded human Pancreas stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Immunohistochemistry-Paraffin-NBP2-53366-img0001.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Immunohistochemistry-Paraffin-NBP2-53366-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Immunohistochemistry-Paraffin-NBP2-53366-img0003.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0015.jpg "Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.")
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1254-img0005.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Affinity Purified ROBO1 antibody was used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ROBO1 Antibody [NB600-1254] - Biotinylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0012.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western blot using Affinity Purified ROBO1 antibody shows detection of a band at ~181 kDa corresponding to ROBO1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0013.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A431 Lysate. Lane 6: A549 Lysate. Lane 7: LNCap Lysate. Lane 8: MOLT-4 Lysate. Lane 9: Ramos Lysate. Lane 10: Raji Lysate. Lane 11: A-172 Lysate. Lane 12: NIH/3T3 Lysate. Load: 35 ug per lane. Primary antibody: ROBO1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted/Observed size: 181kDa for ROBO1.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0014.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0011.jpg)
![ELISA: ROBO1 Antibody [NB600-1254] - ELISA results of purified ROBO1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/ROBO1-Antibody-ELISA-NB600-1254-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: MUC5AC Antibody (CLH2) [NBP2-44455] - Human stomach tissue (FFPE). Antibody at 1:100. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MUC5AC-Antibody-CLH2-Immunocytochemistry-Immunofluorescence-NBP2-44455-img0003.jpg "Immunocytochemistry/Immunofluorescence: MUC5AC Antibody (CLH2) [NBP2-44455] - Human stomach tissue (FFPE). Antibody at 1:100. ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: MUC5AC Antibody (CLH2) [NBP2-44455] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Antibody (CLH2). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty, University of Porto, Portugal.](http://images.novusbio.com/fullsize/MUC5AC-Antibody-CLH2-Immunohistochemistry-Paraffin-NBP2-44455-img0002.jpg)
![Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Western-Blot-NBP2-27208-img0002.jpg "Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.")
![Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Western-Blot-NBP2-79720-img0010.jpg "Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).")
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of paraformaldehyde-Raji cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunocytochemistry-Immunofluorescence-NBP2-79720-img0003.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0008.jpg)
![Flow Cytometry: RPSA Antibody (RPSA/2699) [NBP2-79720] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Flow-Cytometry-NBP2-79720-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of HeLa cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunocytochemistry-Immunofluorescence-NBP2-79720-img0002.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0004.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0005.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0006.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Tonsil stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0007.jpg)
![Protein Array: RPSA Antibody (RPSA/2699) [NBP2-79720] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RPSA Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Protein-Array-NBP2-79720-img0009.jpg)