DYKDDDDK Epitope Tag Antibody (231) Summary
The exact sequence of DYKDDDDK Epitope Tag Antibody (231) is proprietary.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:1000-1:10000
- Immunoprecipitation 1:100-1:500
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Protein G purified
FLAG(R) and ANTI-FLAG(R) are registered trademarks of Sigma-Aldrich Co. LLC.
Alternate Names for DYKDDDDK Epitope Tag Antibody (231)
- DDDDK epitope tag
- DYKDDDDK Epitope Tag
- DYKDDDDK tag
- ECS epitope tag
- ECS tag
- Enterokinase Cleavage Site epitope tag
- Enterokinase Cleavage Site tag
- epitope tag flag
- FLAG (FLAG is a trademark of Sigma Aldrich)
- FLAG Tag
- Ty 1 Tag
- xxxDDDDK epitope tag
Fusion proteins, in which a known peptide such as a His-Tag, GST-Tag, or DYKDDDDK (FLAG(R)) is cloned with the target of interest, were developed to simplify protein isolation. Since specific antibodies may not be available against the protein of interest, epitope tag antibodies are commonly used to detect protein expression.
The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).
1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796
2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294
3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954
FLAG(R) is a trademark of Sigma-Aldrich.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Publications for DYKDDDDK Epitope Tag Antibody (NBP2-43570) (0)
There are no publications for DYKDDDDK Epitope Tag Antibody (NBP2-43570).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for DYKDDDDK Epitope Tag Antibody (NBP2-43570) (0)
There are no reviews for DYKDDDDK Epitope Tag Antibody (NBP2-43570).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for DYKDDDDK Epitope Tag Antibody (NBP2-43570). (Showing 1 - 6 of 6 FAQ).
Are the FLAG Tag antibodies validated in Simple Western?
- Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.
What the theoretical molecular weight for FLAG Tag antibodies?
- The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.
Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
- If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
Will FLAG Tag antibodies recognize FLAG at the C-terminal end?
- Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.
Do FLAG Tag antibodies comes in lyophilized form?
- The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.
What is the immunogen sequence of this FLAG Tag antibody?
- The exact sequence of DYKDDDDK Epitope Tag Antibody (231) is proprietary.