| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43979 | |||
| BSA Free | NBP2-52919-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26877-25ul | |||
| Azide and BSA Free | NBP3-44280 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49516-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26871-25ul | |||
| Azide and BSA Free | NBP3-43916 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81085-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB72272-SP | |||
![Western Blot: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - Analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody (M01), clone 3A9-1A9.Lane 1: HPX transfected lysate (Predicted MW: 28.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Hemopexin-Antibody-3A9-1A9-Western-Blot-H00003263-M01-img0006.jpg "Western Blot: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - Analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody (M01), clone 3A9-1A9.Lane 1: HPX transfected lysate (Predicted MW: 28.6 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - Analysis of HPX over-expressed 293 cell line, cotransfected with HPX Validated Chimera RNAi ( Cat # H00003263-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HPX monoclonal antibody (M01), clone 3A9-1A9 (Cat # H00003263-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Hemopexin-Antibody-3A9-1A9-Western-Blot-H00003263-M01-img0003.jpg)
![Western Blot: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - HPX monoclonal antibody (M01), clone 3A9-1A9. Analysis of HPX expression in human liver.](http://images.novusbio.com/fullsize/Hemopexin-Antibody-3A9-1A9-Western-Blot-H00003263-M01-img0005.jpg)
![Immunoprecipitation: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - Analysis of HPX transfected lysate using anti-HPX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HPX MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Hemopexin-Antibody-3A9-1A9-Immunoprecipitation-H00003263-M01-img0002.jpg)
![Sandwich ELISA: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - Detection limit for recombinant GST tagged HPX is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hemopexin-Antibody-3A9-1A9-Sandwich-ELISA-H00003263-M01-img0004.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0344) [NBP2-52919] - Staining in human placenta and tonsil tissues. Corresponding FLT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-CL0344-Immunohistochemistry-Paraffin-NBP2-52919-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0344) [NBP2-52919] - Staining in human placenta and tonsil tissues. Corresponding FLT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0344) [NBP2-52919] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-CL0344-Immunohistochemistry-Paraffin-NBP2-52919-img0005.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0344) [NBP2-52919] - Staining of human kidney shows strong cytoplasmic positivity in endothelial cells.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-CL0344-Immunohistochemistry-Paraffin-NBP2-52919-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0344) [NBP2-52919] - Staining of human small intestine shows strong cytoplasmic positivity in endothelial cells, as well as positivity in smooth muscle.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-CL0344-Immunohistochemistry-Paraffin-NBP2-52919-img0007.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0344) [NBP2-52919] - Staining of human tonsil shows no cytoplasmic positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-CL0344-Immunohistochemistry-Paraffin-NBP2-52919-img0008.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Analysis in human placenta and pancreas tissues. Corresponding ENG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Analysis in human placenta and pancreas tissues. Corresponding ENG RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human testis shows moderate membranous positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0008.jpg)
![Immunohistochemistry: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human placenta shows membranous positivity in trophoblasts.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-NBP2-49516-img0001.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0006.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0007.jpg)
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-1572024145956..jpg "Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE-1 antibody at 1/1,000 dilution.Lane 1: A549-si NT cell lysateLane 2: A549-si LYVE-1 cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-66940 was shown to specifically react with LYVE-1 in A549-si NT cells. Weakened band was observed when A549-si LYVE-1 sample was tested. A549-si NT and Hela-si LYVE-1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-15720241537..jpg)
![Flow Cytometry: LYVE-1 Antibody (JF0979) [NBP2-66940] - Analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/LYVE-1-Antibody-JF0979-Flow-Cytometry-NBP2-66940-img0001.jpg)
![Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-278202416331211.jpg "Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32145) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-298202414115443.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling CD31/PECAM-1 with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-29202410484878.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81085-img0001.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)