| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38316-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35747-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90185-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50537-0.025ml | |||
![Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Chk2 [p Thr68] Antibody [NBP3-13076] - Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chk2 (phospho Thr68) stained by Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13076-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Chk2 [p Thr68] Antibody [NBP3-13076] - Wild-type (WT) and Chk2 (phospho Thr68) knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0003.jpg)
![Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Ser2056]-Antibody-Western-Blot-NBP3-13142-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - DNA-PKcs (phospho Ser2056) antibody detects DNA-PKcs (phospho Ser2056) protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA-PKcs (phospho Ser2056) stained by DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Ser2056]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13142-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Ser2056]-Antibody-Western-Blot-NBP3-13142-img0002.jpg)
![Western Blot: PP2A alpha Antibody [NB100-2260] - Detection of Human and Mouse PP2A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NB100-2260-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: PP2A alpha Antibody [NB100-2260] - Detection of Human and Mouse PP2A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: PP2A alpha Antibody [NB100-2260] - Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 (lot 2) used for IP at 6 ug per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-PP2A antibody NB100-2259. For blotting immunoprecipitated PP2A, NB100-2260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunoprecipitation-NB100-2260-img0004.jpg)
![Western Blot: Aurora A Antibody (35C1) [NB100-1641] - Analysis using the Biotin conjugate of NB100-1641. Detection of recombinant Aurora-A kinase protein probed with 100-1829 and F(ab')2 rabbit anti-mouse IgG:HRP.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-35C1-Western-Blot-NB100-1641-img0002.jpg "Western Blot: Aurora A Antibody (35C1) [NB100-1641] - Analysis using the Biotin conjugate of NB100-1641. Detection of recombinant Aurora-A kinase protein probed with 100-1829 and F(ab')2 rabbit anti-mouse IgG:HRP.")
![Western Blot: RPA14 Antibody [NBP3-38316] - Western blot analysis of various lysates using RPA14 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38316_rabbit-rpa14-pab-101220241136013.jpg "Western Blot: RPA14 Antibody [NBP3-38316] - Western blot analysis of various lysates using RPA14 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Immunocytochemistry/ Immunofluorescence: RPA14 Antibody [NBP3-38316] - Immunofluorescence analysis of MCF7 cells using RPA14 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-38316_rabbit-rpa14-pab-1012202411421017.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP3-35747] - Western blot analysis of lysates from PC-3 cells, using DNA Ligase IV Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35747_rabbit-dna-ligase-iv-pab-91220241418132.jpg "Western Blot: DNA Ligase IV Antibody [NBP3-35747] - Western blot analysis of lysates from PC-3 cells, using DNA Ligase IV Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: DNA Ligase IV Antibody [NBP3-35747] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DNA Ligase IV Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35747_rabbit-dna-ligase-iv-pab-9122024141817.jpg)
![Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-05444] - Western blot analysis of extracts of HeLa cells, using DNA-PKcs antibody (NBP3-05444). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Ser2056]-Antibody-Western-Blot-NBP3-05444-img0002.jpg "Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-05444] - Western blot analysis of extracts of HeLa cells, using DNA-PKcs antibody (NBP3-05444). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/Immunofluorescence: DNA-PKcs [p Ser2056] Antibody [NBP3-05444] - Immunofluorescence analysis of U2OS cells using DNA-PKcs antibody (NBP3-05444) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Ser2056]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05444-img0001.jpg)
![Immunohistochemistry: DNA-PKcs [p Ser2056] Antibody - Azide and BSA Free [DNA-PKcs] - Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using DNA-PKcs Rabbit pAb at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05444_rabbit-polyclonal-dna-pkcs-p-ser2056-antibody-5220251639681.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0003.jpg "Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human squamous cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody (001) [NBP2-90185] - Staining of RRM2B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90185 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90185_rabbit-monoclonal-p53r2-antibody-001-235202514492316.png)