| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61694-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76741-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38316-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90185-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26493-50ul | |||
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0003.jpg "Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against human AURKA (AA: 268-404) recombinant protein. (Expected MW is 41.5 kDa)](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0002.jpg)
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mouse mAb against HEK293 (1), PC-12 (2), and HT-29 (3) cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0004.jpg)
![ELISA: Aurora A Antibody (4H5A8) [NBP2-61694] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-ELISA-NBP2-61694-img0001.jpg)
![Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 85 kDa<br/> Observed band size: 85 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-278202416285244.jpg "Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 85 kDa<br/> Observed band size: 85 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MLH1 Antibody (PD01-61) [NBP3-32599] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-298202413585516.jpg)
![Western blot analysis on different lysates with NBP3-32599 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate (negative) Lane 3: A549 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32599 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32599_-western-blot-639204594554880496.jpg)
![Western blot analysis on different lysates with NBP3-32599 at 1/2,000 dilution. Lane 1: C2C12 cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32599 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32599_-western-blot-639204597795571731.jpg)
![Western Blot: MCM4 Antibody [NB100-290] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.](http://images.novusbio.com/fullsize/MCM4-Antibody-Western-Blot-NB100-290-img0002.jpg "Western Blot: MCM4 Antibody [NB100-290] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.")
![Immunohistochemistry: MCM4 Antibody [NB100-290] - Sample: FFPE section of human testicular seminoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-NB100-290-img0009.jpg)
![Immunohistochemistry;HMGN1/HMG14 Antibody[NBP3-29996]Detection of human and mouse HMGN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma and mouse teratoma . Antibody: Affinity purified rabbit anti-HMGN1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29997_rabbit-hmgn1-hmg14-pab-78202410372613.jpg "Immunohistochemistry;HMGN1/HMG14 Antibody[NBP3-29996]Detection of human and mouse HMGN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma and mouse teratoma . Antibody: Affinity purified rabbit anti-HMGN1 used at a dilution of 1:5,000 . Detection: DAB")
![Immunocytochemistry/ Immunofluorescence;HMGN1/HMG14 Antibody[NBP3-30724]Detection of human HMGN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-HMGN1 used at a dilution of 1:2,000 . . Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100 .](http://images.novusbio.com/fullsize/nbp3-29997_rabbit-hmgn1-hmg14-pab-78202493618.jpg)
![Immunohistochemistry;SMC1 Antibody[NBP3-29562]Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29588_rabbit-smc1-pab-7820241037260.jpg "Immunohistochemistry;SMC1 Antibody[NBP3-29562]Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 . Detection: DAB")
![Immunohistochemistry;SMC1 Antibody[NBP3-29588]Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29588_rabbit-smc1-pab-78202410392619.jpg)
![Immunoprecipitation:SMC1 AntibodyNBP3-29588] -Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC1 antibody NBP3-29588 (lot NBP3-29588-6) used for IP at 6 µg per reaction. SMC1 was also immunoprecipitated by rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (A700-018). For blotting immunoprecipitated SMC1, A700-018 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29588_rabbit-smc1-pab-301220242025111.jpg)
![Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0006.jpg "Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: PERP Antibody [NBP1-76741] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug lysates per lane. Antibodies: PERP NBP1-76741 (1ug/ml), PERP antibody from Company B (2 ug/ml), beta-actin NBP1-76692 (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0004.jpg)
![Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] - Validation of PERP in A431 Cells. Immunocytochemical analysis of A431 cells using anti-PEPR antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76741_rabbit-polyclonal-perp-antibody-271120241923644.jpg)
![Western Blot: Rad51C Antibody [NB100-1127] - Western blot analysis of Rad51C in HeLa lysate using NB100-1127 at 2 ug/ ml.](http://images.novusbio.com/fullsize/Rad51C-Antibody-Western-Blot-NB100-1127-img0004.jpg "Western Blot: Rad51C Antibody [NB100-1127] - Western blot analysis of Rad51C in HeLa lysate using NB100-1127 at 2 ug/ ml.")
![Western Blot: Rad51C Antibody [NB100-1127] - (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/Rad51C-Antibody-Western-Blot-NB100-1127-img0002.jpg)
![Western Blot: RPA14 Antibody [NBP3-38316] - Western blot analysis of various lysates using RPA14 Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38316_rabbit-rpa14-pab-101220241136013.jpg "Western Blot: RPA14 Antibody [NBP3-38316] - Western blot analysis of various lysates using RPA14 Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.")
![Immunocytochemistry/ Immunofluorescence: RPA14 Antibody [NBP3-38316] - Immunofluorescence analysis of MCF7 cells using RPA14 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-38316_rabbit-rpa14-pab-1012202411421017.jpg)
![Immunohistochemistry: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-DNA-PKcs antibody (NBP3-32272) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32272) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32272_mouse-dna-pkcs-mab-2-b3-r-298202414333675.jpg "Immunohistochemistry: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-DNA-PKcs antibody (NBP3-32272) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32272) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Immunocytochemistry analysis of HepG2 cells labeling DNA-PKcs with Rabbit anti-DNA-PKcs antibody (NBP3-32272) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DNA-PKcs antibody (NBP3-32272) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32272_mouse-dna-pkcs-mab-2-b3-r-29202410334012.jpg)
![Western blot analysis on different lysates with NBP3-32272 at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lane 6: HT-29 cell lysate Lane 7: A549 cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 469 kDa Observed band size: 469 kDa Exposure time: 23 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32272 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32272_-western-blot-639204590077132238.jpg)
![Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-298202414262624.jpg "Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32755 at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32755 at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32755_-western-blot-639204597483485812.jpg)
![Western blot analysis on different lysates with NBP3-32755 at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with UV for 40 minutes add 1mM Sodium orthovanadate and recovery for 30 minutes cell lysate Lane 3: L-929 cell lysate Lane 4: L-929 treated with UV for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32755 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32755_-western-blot-639204598086390798.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0003.jpg "Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human squamous cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody (001) [NBP2-90185] - Staining of RRM2B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90185 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90185_rabbit-monoclonal-p53r2-antibody-001-235202514492316.png)
![Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate.<br /> Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).<br /> Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug).<br /> Lane 3: PC3 whole cell lysate (20ug).<br />](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-262202413221524.jpg "Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate.<br /> Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).<br /> Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug).<br /> Lane 3: PC3 whole cell lysate (20ug).<br />")
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-26220241547561.jpg)
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-2722024845325.jpg)
![Western Blot: ATM Antibody (4E11) [NBP3-26493] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate.<br /> All lanes: ATM Antibody at 2.05ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 350 KDa<br /> Observed band size: 350 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-28220241140928.jpg)