| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98947-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89207-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14796-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90081-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12019-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20096-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75189-0.025mg | |||
![Western Blot: TIPIN Antibody [NBP2-98947] - Lane A: HCT116 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:50 kDa](http://images.novusbio.com/fullsize/TIPIN-Antibody-Western-Blot-NBP2-98947-img0003.jpg "Western Blot: TIPIN Antibody [NBP2-98947] - Lane A: HCT116 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:50 kDa")
![Immunoprecipitation: TIPIN Antibody [NBP2-98947] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-TIPIN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TIPIN rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :53 kDa](http://images.novusbio.com/fullsize/TIPIN-Antibody-Immunoprecipitation-NBP2-98947-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TIPIN Antibody [NBP2-98947] - Immunofluorescence staining of TIPIN in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98947 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98947_rabbit-polyclonal-tipin-antibody-235202511354133.png)
![Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89207_rabbit-monoclonal-4ebp1-antibody-007-23520251154563.png "Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.")
![Immunohistochemistry: RBM14 Antibody [NBP3-14796] - Immunochemical staining of human RBM14 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-NBP3-14796-img0003.jpg "Immunohistochemistry: RBM14 Antibody [NBP3-14796] - Immunochemical staining of human RBM14 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry: RBM14 Antibody [NBP3-14796] - Immunochemical staining of human RBM14 in human placenta with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-NBP3-14796-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RBM14 Antibody [NBP3-14796] - Immunofluorescence staining of RBM14 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-14796 antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-14796_rabbit-rbm14-pab-235202521451788.png)
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0003.jpg "Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human tonsil with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human placenta with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (009) [NBP2-90081] - Staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90081 antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).](http://images.novusbio.com/fullsize/nbp2-90081_rabbit-monoclonal-hsf1-antibody-009-235202514492390.png)
![Immunocytochemistry/Immunofluorescence: ZMYM3 Antibody (C.15200016) [NBP2-59244] - HeLa cells were stained with the antibody against ZMYM3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/Triton X-100 containing 1% BSA. The cells were immunofluorescently labeled with the ZMYM3 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/ZMYM3-Antibody-C-15200016-Immunofluorescence-NBP2-59244-img0002.jpg "Immunocytochemistry/Immunofluorescence: ZMYM3 Antibody (C.15200016) [NBP2-59244] - HeLa cells were stained with the antibody against ZMYM3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/Triton X-100 containing 1% BSA. The cells were immunofluorescently labeled with the ZMYM3 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: ZMYM3 Antibody (C.15200016) [NBP2-59244] - ChIP was performed using HepG2 cells, the antibody against ZMYM3 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analyzed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers near the CAMK2N1 and SCML1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/ZMYM3-Antibody-C-15200016-Chromatin-Immunoprecipitation-NBP2-59244-img0001.jpg)
![Protein Array: ZMYM3 Antibody (C.15200016) [NBP2-59244] - Protein array analysis with the antibody against ZMYM3 was demonstrated using the HuProt human protein microarray (CDI Laboratories), a protein array containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. Figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 3 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for ZMYM3.](http://images.novusbio.com/fullsize/ZMYM3-Antibody-C-15200016-Protein-Array-NBP2-59244-img0003.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (C.15200219) [NBP2-59242] - Histone extracts (15 ug) from HeLa cells were analysed using the antibody against H4K16ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-C-15200219-Western-Blot-NBP2-59242-img0001.jpg "Western Blot: Histone H4 [ac Lys16] Antibody (C.15200219) [NBP2-59242] - Histone extracts (15 ug) from HeLa cells were analysed using the antibody against H4K16ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys16] Antibody (C.15200219) [NBP2-59242] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K16ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis)](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-C-15200219-Chromatin-Immunoprecipitation-NBP2-59242-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys16] Antibody (C.15200219) [NBP2-59242] - To test the cross reactivity of the antibody against H4K16ac, analysis was performed with peptides containing other acetylations and unmodified sequences of histone H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-C-15200219-Dot-Blot-NBP2-59242-img0004.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K8ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Western-Blot-NBP2-59240-img0001.jpg "Western Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K8ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Analysis was performed using HeLa cells, the monoclonal antibody against H4K8ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the EIF4A2 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Chromatin-Immunoprecipitation-NBP2-59240-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - To test the cross reactivity of the antibody against H4K8ac, analysis was performed with peptides containing other acetylations and unmodified sequences of histone H4. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Dot-Blot-NBP2-59240-img0004.jpg)
![Western Blot: DNMT3B Antibody [NBP2-59291] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Western-Blot-NBP2-59291-img0001.jpg "Western Blot: DNMT3B Antibody [NBP2-59291] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: DNMT3B Antibody [NBP2-59291] - Human HeLa cells were stained with the antibody against DNMT3B and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the DNMT3B antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunofluorescence-NBP2-59291-img0003.jpg)
![ELISA: DNMT3B Antibody [NBP2-59291] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody directed against DNMT3B. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:220,000.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-ELISA-NBP2-59291-img0002.jpg)
![Western Blot: p97/VCP Antibody (2B2) [H00007415-M18] - Analysis of VCP expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/p97-VCP-Antibody-2B2-Western-Blot-H00007415-M18-img0005.jpg "Western Blot: p97/VCP Antibody (2B2) [H00007415-M18] - Analysis of VCP expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (2B2) [H00007415-M18] - Analysis of monoclonal antibody to VCP on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/p97-VCP-Antibody-2B2-Immunocytochemistry-Immunofluorescence-H00007415-M18-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric [NBP3-12019] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of KM-2 (NBP3-12019) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12019, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-ac-Lys16-ac-Lys8-Antibody-KM-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12019-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric [NBP3-12019] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of KM-2 (NBP3-12019) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12019, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric - Azide and BSA Free [NBP3-12019] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12019 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12019_rabbit-monoclonal-histone-h4-ac-lys12-ac-lys16-ac-lys8-antibody-km-2-chimeric-74202522113023.png)
![Flow Cytometry: hHR23b Antibody (SAIC-28A-29) - Azide and BSA Free [NBP3-20096] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-28A-29 (NBP3-20096, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20096_rabbit-hhr23b-mab-saic-28a-29-azide-and-bsa-free-2742023924299.png "Flow Cytometry: hHR23b Antibody (SAIC-28A-29) - Azide and BSA Free [NBP3-20096] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-28A-29 (NBP3-20096, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28A-29) - Azide and BSA Free [NBP3-20096] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28A-29 (NBP3-20096) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nucleoli staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20096, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20096_rabbit-hhr23b-mab-saic-28a-29-azide-and-bsa-free-274202392426.png)
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0003.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Eptratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0001.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Cynomolgus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0002.jpg)