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![Western Blot: Nbs1 Antibody (4G4R1) [NBP3-16355] - Western blot analysis of extracts of PC-3 cells, using Nbs1 Rabbit mAb (NBP3-16355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Nbs1-Antibody-4G4R1-Western-Blot-NBP3-16355-img0001.jpg "Western Blot: Nbs1 Antibody (4G4R1) [NBP3-16355] - Western blot analysis of extracts of PC-3 cells, using Nbs1 Rabbit mAb (NBP3-16355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: Nbs1 Antibody (4G4R1) [NBP3-16355] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Nbs1 antibody (NBP3-16355). Western blot was performed from the immunoprecipitate using Nbs1 antibody (NBP3-16355) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Nbs1-Antibody-4G4R1-Immunoprecipitation-NBP3-16355-img0002.jpg)
![Western Blot: ATRIP Antibody (OTI1G6) - Azide and BSA Free [NBP2-72270] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATRIP.](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI1G6-Azide-and-BSA-Free-Western-Blot-NBP2-72270-img0002.jpg "Western Blot: ATRIP Antibody (OTI1G6) - Azide and BSA Free [NBP2-72270] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATRIP.")
![Western Blot: ATRIP Antibody (OTI1G6) - Azide and BSA Free [NBP2-72270] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ATRIP-Antibody-OTI1G6-Azide-and-BSA-Free-Western-Blot-NBP2-72270-img0001.jpg)
![Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32890_rabbit-rad50-mab-je60-04-288202412461633.jpg "Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Chk2 [p Thr68] Antibody (JE43-12) [NBP3-32756] - Western blot analysis of Chk2 on different lysates with Rabbit anti-Chk2 antibody (NBP3-32756) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20uM Etoposide for 2 hours cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 25uM Etoposide for 5 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32756) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32756_rabbit-chk2-p-thr68-mab-je43-12-288202412442927.jpg "Western Blot: Chk2 [p Thr68] Antibody (JE43-12) [NBP3-32756] - Western blot analysis of Chk2 on different lysates with Rabbit anti-Chk2 antibody (NBP3-32756) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20uM Etoposide for 2 hours cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 25uM Etoposide for 5 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32756) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32754) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32754) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32754_rabbit-chk1-p-ser280-mab-je47-66-288202412442937.jpg "Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32754) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32754) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: BRCA1 Antibody (A7E3-R) [NBP3-32090] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-BRCA1 antibody (NBP3-32090) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32090) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32090_mouse-brca1-mab-a7e3-r-298202414304150.jpg "Immunohistochemistry: BRCA1 Antibody (A7E3-R) [NBP3-32090] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-BRCA1 antibody (NBP3-32090) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32090) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: H2AX Protein [NBP1-42464] - The anti-H2AX antibody NB100-638 was used to detect this protein. 200ng of protein was loaded onto the gel and NB100-638 was used to detect the protein.](http://images.novusbio.com/fullsize/H2AX-Protein-Western-Blot-NBP1-42464-img0002.jpg "Western Blot: H2AX Protein [NBP1-42464] - The anti-H2AX antibody NB100-638 was used to detect this protein. 200ng of protein was loaded onto the gel and NB100-638 was used to detect the protein.")
![SDS-Page: H2AX Protein [NBP1-42464] - This H2AX protein was tested on a 4-20% SDS-PAGE gel with 5ug of protein loaded on the gel. The purity of the protein is >95%.](http://images.novusbio.com/fullsize/H2AX-Protein-Immunoblotting-NBP1-42464-img0001.jpg)
![Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.](http://images.novusbio.com/fullsize/p53-Antibody-V5P4H6-B3-Immunohistochemistry-NBP2-50231-img0001.jpg "Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.")
![Protein Array: BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free [NBP2-54337] - Protein Array containing more than 19,000 full-length human proteins using BRCA-1 antibody (BRCA1/1472). The Z-score represents the strength of a signal that a monoclonal antibody, mAb, (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein. Z-scores are in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/BRCA1-Antibody-BRCA1-1472-Azide-and-BSA-Free-Protein-Array-NBP2-54337-img0002.jpg "Protein Array: BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free [NBP2-54337] - Protein Array containing more than 19,000 full-length human proteins using BRCA-1 antibody (BRCA1/1472). The Z-score represents the strength of a signal that a monoclonal antibody, mAb, (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein. Z-scores are in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.")