| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54661-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38677-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85108-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54659-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26030 | |||
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414161884.jpg "Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32600) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414333686.jpg)
![Immunocytochemistry/ Immunofluorescence: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunocytochemistry analysis of A431 cells labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-292024111485.jpg)
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)
![Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-298202414262624.jpg "Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-392024848501.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] - Immunocytochemistry of HeLa cells using Clone RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26030_rabbit-histone-h2ax-mab-rm224-azide-and-bsa-free-22620242311083.png "Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] - Immunocytochemistry of HeLa cells using Clone RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Western Blot: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV, using RM224 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26030_rabbit-histone-h2ax-mab-rm224-azide-and-bsa-free-24620241332456.png)
![Western Blot: Nbs1 Antibody [NBP2-54661] - Analysis using Nbs1 Antibody [NBP2-54661]. Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Molecular Weight observed at ~ 95 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NBP2-54661-img0004.jpg "Western Blot: Nbs1 Antibody [NBP2-54661] - Analysis using Nbs1 Antibody [NBP2-54661]. Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Molecular Weight observed at ~ 95 kDa.")
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP2-54661] - Staining of human testis with Nbs1 Antibody [NBP2-54661] shows strong nuclear positivity in cells of seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-Paraffin-NBP2-54661-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody [NBP2-38677] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MLH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38677-img0003.jpg "Immunocytochemistry/Immunofluorescence: MLH1 Antibody [NBP2-38677] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: MLH1 Antibody [NBP2-38677] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/MLH1-Antibody-Immunohistochemistry-NBP2-38677-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p53 DINP1 Antibody [NBP1-85108] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85108-img0004.jpg "Immunocytochemistry/Immunofluorescence: p53 DINP1 Antibody [NBP1-85108] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.")
![Immunohistochemistry-Paraffin: p53 DINP1 Antibody [NBP1-85108] - Staining of human cerebral cortex shows nuclear positivity in both neuronal and glial cells.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-Immunohistochemistry-Paraffin-NBP1-85108-img0005.jpg)
![Western Blot: 53BP1 Antibody [NBP2-54659] - Western blot analysis of human cell line SCLC-21H, blotted with 53BP1 Antibody. The molecular weight is observed at ~160 kDa and the theoretical molecular weight of the endogenous protein is 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody-Western-Blot-NBP2-54659-img0005.jpg "Western Blot: 53BP1 Antibody [NBP2-54659] - Western blot analysis of human cell line SCLC-21H, blotted with 53BP1 Antibody. The molecular weight is observed at ~160 kDa and the theoretical molecular weight of the endogenous protein is 214 kDa.")
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NBP2-54659] - Staining of human cell line U-2 OS with 53BP1 Antibody shows localization to nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54659-img0003.jpg)
![Western Blot: Histone H2AX [p Ser 119] Antibody (RM224) [NBP3-18533] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using NBP3-18533 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H2AX-p-Ser-119-Antibody-RM224-Western-Blot-NBP3-18533-img0001.jpg "Western Blot: Histone H2AX [p Ser 119] Antibody (RM224) [NBP3-18533] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using NBP3-18533 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.")
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-18533] - Immunocytochemistry of HeLa cells using Clone RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-18533_rabbit-histone-h2ax-p-ser-139-mab-rm224-immunocytochemistry-immunofluorescence-189202523444711.png)