| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38093-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02946-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2649-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-12189-0.025ml | |||
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Western Blot: LYPLA1 Antibody [NBP3-38093] - Western Blot analysis of various lysates using LYPLA1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38093_rabbit-lypla1-pab-3112025177010.jpg "Western Blot: LYPLA1 Antibody [NBP3-38093] - Western Blot analysis of various lysates using LYPLA1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: LYPLA1 Antibody [NBP3-38093] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using LYPLA1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38093_rabbit-lypla1-pab-3112025173174.jpg)
![Western Blot: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of extracts of various cell lines, using HMGN1/HMG14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Western-Blot-NBP3-02946-img0004.jpg "Western Blot: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of extracts of various cell lines, using HMGN1/HMG14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit")
![Immunocytochemistry/Immunofluorescence: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of HeLa cells using HMGN1/HMG14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02946-img0001.jpg)
![Western Blot: Connexin 43/GJA1 Antibody [NB300-309] - Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.](http://images.novusbio.com/fullsize/Connexin-43-GJA1-Antibody-Western-Blot-NB300-309-img0003.jpg "Western Blot: Connexin 43/GJA1 Antibody [NB300-309] - Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.")
![Western Blot: PRMT1 Antibody (PRMT1-171) - BSA Free [NB120-12189] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-PRMT1 Clone: PRMT1-171. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. NRK 5. MDBK 6. MDCK 7. CHO 8. 3T3](http://images.novusbio.com/fullsize/PRMT1-Antibody-PRMT1-171-BSA-Free-Western-Blot-NB120-12189-img0003.jpg "Western Blot: PRMT1 Antibody (PRMT1-171) - BSA Free [NB120-12189] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-PRMT1 Clone: PRMT1-171. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. NRK 5. MDBK 6. MDCK 7. CHO 8. 3T3")
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Western Blot: Kif4A Antibody (JE38-91) [NBP3-32502] - Western blot analysis of Kif4A on different lysates with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: SW480 cell lysate (20 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Predicted band size: 140 kDa Observed band size: 160 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32502_rabbit-kif4a-mab-je38-91-27820241631627.jpg "Western Blot: Kif4A Antibody (JE38-91) [NBP3-32502] - Western blot analysis of Kif4A on different lysates with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: SW480 cell lysate (20 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Predicted band size: 140 kDa Observed band size: 160 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Kif4A Antibody (JE38-91) [NBP3-32502] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32502_rabbit-kif4a-mab-je38-91-29820241492699.jpg)
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0002.jpg "Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with feline leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0001.jpg)
![Western Blot: Connexin 43/GJA1 Antibody (M496) [NBP3-23240] - Western blot analysis of Connexin 43/GJA1 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-Connexin 43/GJA1 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23240_mouse-connexin-43-gja1-mab-m496-199202317594412.jpg "Western Blot: Connexin 43/GJA1 Antibody (M496) [NBP3-23240] - Western blot analysis of Connexin 43/GJA1 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-Connexin 43/GJA1 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).")
![Western Blot: Catenin alpha 1 Antibody (1G5) - BSA Free [NBP3-43548] - A431(A) (0.003µg/ml), HeLa(B) (0.03ug/ml) and MCF7(C) (0.01ug/ml) cell lysate. (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-43548 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43548_mouse-catenin-alpha-1-mab-1g5-azide-and-bsa-free-western-blot-2220260322019.png "Western Blot: Catenin alpha 1 Antibody (1G5) - BSA Free [NBP3-43548] - A431(A) (0.003µg/ml), HeLa(B) (0.03ug/ml) and MCF7(C) (0.01ug/ml) cell lysate. (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-43548 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/ Immunofluorescence: Catenin alpha 1 Antibody (1G5) - BSA Free [NBP3-43548] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on coverslips stained with NBP3-43548 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43548, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43548_mouse-catenin-alpha-1-mab-1g5-azide-and-bsa-free-immunocytochemistry-immunofluorescence-222026035115.png)
![Western Blot: Catenin alpha 1 Antibody (1G5) - Chimeric - BSA Free [NBP3-43549] - A431(A) (0.003ug/ml), HeLa(B) (0.03ug/ml) and MCF7(C) (0.01ug/ml) cell lysate. (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-43549 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43549_rabbit-catenin-alpha-1-mab-1g5-chimeric-azide-bsa-free-7420252226529.png "Western Blot: Catenin alpha 1 Antibody (1G5) - Chimeric - BSA Free [NBP3-43549] - A431(A) (0.003ug/ml), HeLa(B) (0.03ug/ml) and MCF7(C) (0.01ug/ml) cell lysate. (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-43549 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: Catenin alpha 1 Antibody (1G5) - Chimeric - BSA Free [NBP3-43549] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP3-43549 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43549, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43549_rabbit-catenin-alpha-1-mab-1g5-chimeric-azide-bsa-free-74202522113031.png)