| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90093-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99560-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98988-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98294-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97782-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98832-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98781-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00356-50ul | |||
![Western Blot: CD133 Antibody (A8C3-R) [NBP3-32124] - Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (NBP3-32124) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32124) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32124_mouse-cd133-mab-a8c3-r-27820241631670.jpg "Western Blot: CD133 Antibody (A8C3-R) [NBP3-32124] - Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (NBP3-32124) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32124) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD133 Antibody (A8C3-R) [NBP3-32124] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (NBP3-32124) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32124) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32124_mouse-cd133-mab-a8c3-r-298202414161867.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880] - Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (NBP3-32880) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: K562 cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32880) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32880_rabbit-pyruvate-dehydrogenase-e2-mab-je34-38-288202412442946.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880] - Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (NBP3-32880) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: K562 cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32880) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (NBP3-32880) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32880) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32880_rabbit-pyruvate-dehydrogenase-e2-mab-je34-38-29820241420919.jpg)
![Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (010) [NBP2-90093] - Immunofluorescence staining of Human AK3L1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90093 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90093_rabbit-monoclonal-ak3l1-antibody-010-235202514492370.png "Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (010) [NBP2-90093] - Immunofluorescence staining of Human AK3L1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90093 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Immunoprecipitation: AK3L1 Antibody (010) [NBP2-90093] - AK4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP2-90093 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP2-90093, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa](http://images.novusbio.com/fullsize/nbp2-90093_rabbit-monoclonal-ak3l1-antibody-010-235202516335030.png)
![Western Blot: AK3L1 Antibody (010) [NBP2-90093] - NBP2-90093 at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:26 kDa](http://images.novusbio.com/fullsize/nbp2-90093_rabbit-monoclonal-ak3l1-antibody-010-235202514492360.png)
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes cell lysate Lane 3: A431 treated with 100ng/mL EGF for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: SK-Br-3 cell lysate Lane 5: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-288202412442922.jpg "Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes cell lysate Lane 3: A431 treated with 100ng/mL EGF for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: SK-Br-3 cell lysate Lane 5: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Immunocytochemistry analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-29202410484847.jpg)
![Flow Cytometry: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Flow cytometric analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32768, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-2920241637217.jpg)
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Western Blot: ATP5O Antibody [NBP2-99560] - Anti-ATP5O rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: COS7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa](http://images.novusbio.com/fullsize/ATP5O-Antibody-Western-Blot-NBP2-99560-img0004.jpg "Western Blot: ATP5O Antibody [NBP2-99560] - Anti-ATP5O rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: COS7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa")
![Immunohistochemistry-Paraffin: ATP5O Antibody [NBP2-99560] - Immunochemical staining of human ATP5O in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP5O-Antibody-Immunohistochemistry-Paraffin-NBP2-99560-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5O Antibody [NBP2-99560] - Immunochemical staining of human ATP5O in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP5O-Antibody-Immunohistochemistry-Paraffin-NBP2-99560-img0002.jpg)
![Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CDCA3-Antibody-Immunohistochemistry-Paraffin-NBP2-98988-img0003.jpg "Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CDCA3-Antibody-Immunohistochemistry-Paraffin-NBP2-98988-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CDCA3 Antibody [NBP2-98988] - Immunofluorescence staining of CDCA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98988 antibody (dilution ratio: 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98988_rabbit-polyclonal-cdca3-antibody-235202521451716.png)
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98294-img0003.jpg "Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98294-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody [NBP2-98294] - Immunofluorescence staining of mCRABP2 in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98294 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98294_rabbit-polyclonal-crabp2-antibody-23520251129330.png)
![Western Blot: RPA14 Antibody [NBP2-97782] - Anti-RPA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: HL-60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa](http://images.novusbio.com/fullsize/RPA14-Antibody-Western-Blot-NBP2-97782-img0004.jpg "Western Blot: RPA14 Antibody [NBP2-97782] - Anti-RPA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: HL-60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa")
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP2-97782] - Immunochemical staining of human RPA3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP2-97782-img0003.jpg)
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP2-97782] - Immunochemical staining of human RPA3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP2-97782-img0002.jpg)
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/TTK-Antibody-Immunohistochemistry-Paraffin-NBP2-98832-img0002.jpg "Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).")
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human RPS27 in human lung cancer with NBP2-98832 (1:1000).](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-235202511444413.png)
![Immunocytochemistry/Immunofluorescence: TTK Antibody [NBP2-98832] - Immunofluorescence staining of TTK in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98832 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-23520252135167.png)
![Western Blot: NCAPH Antibody [NBP2-98781] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:82 kDaObserved band size:110 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP2-98781-img0001.jpg "Western Blot: NCAPH Antibody [NBP2-98781] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:82 kDaObserved band size:110 kDa")
![Immunoprecipitation: NCAPH Antibody [NBP2-98781] - Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-NCAPH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NCAPH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 83 kDaObserved band size: 110 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunoprecipitation-NBP2-98781-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP2-98781] - Staining of NCAPH in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-98781 antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98781_rabbit-polyclonal-ncaph-antibody-23520251135415.png)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP3-00356] - NBP3-00356 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:34 kDa](http://images.novusbio.com/fullsize/nbp3-00356_rabbit-polyclonal-pa28-activator-gamma-subunit-psme3-antibody-23520251154564.png "Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP3-00356] - NBP3-00356 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:34 kDa")
![Immunoprecipitation: PA28 Activator gamma Subunit/PSME3 Antibody [NBP3-00356] - PSME3 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg Jurkat Whole Cell Lysate 4 uL NBP3-00356 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00356, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size: 33 kDa](http://images.novusbio.com/fullsize/nbp3-00356_rabbit-polyclonal-pa28-activator-gamma-subunit-psme3-antibody-23520251220553.png)
![Immunocytochemistry/ Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP3-00356] - Immunofluorescence staining of PA28 Activator gamma Subunit/PSME3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00356 (dilution ratio 1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp3-00356_rabbit-polyclonal-pa28-activator-gamma-subunit-psme3-antibody-23520252135126.png)