| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89207-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98888-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89187-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12640-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89878-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90093-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98758-50ul | |||
![Western Blot: MSH2 Antibody (10G2-R) [NBP3-32605] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: HEK-293 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: A431 cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: SW480 cell lysate (15 ug/Lane) Lane 7: PC-3M cell lysate (15 ug/Lane) Lane 8: LNCaP cell lysate (negative) (15 ug/Lane) Lane 9: LoVo cell lysate (negative) (15 ug/Lane) Lane 10: NIH/3T3 cell lysate (15 ug/Lane) Lane 11: RAW264.7 cell lysate (15 ug/Lane) Lane 12: PC-12 cell lysate (15 ug/Lane) Lane 13: Mouse testis tissue lysate (25 ug/Lane) Lane 14: Rat testis tissue lysate (25 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32605_mouse-msh2-mab-10g2-r-27820241631687.jpg "Western Blot: MSH2 Antibody (10G2-R) [NBP3-32605] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: HEK-293 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: A431 cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: SW480 cell lysate (15 ug/Lane) Lane 7: PC-3M cell lysate (15 ug/Lane) Lane 8: LNCaP cell lysate (negative) (15 ug/Lane) Lane 9: LoVo cell lysate (negative) (15 ug/Lane) Lane 10: NIH/3T3 cell lysate (15 ug/Lane) Lane 11: RAW264.7 cell lysate (15 ug/Lane) Lane 12: PC-12 cell lysate (15 ug/Lane) Lane 13: Mouse testis tissue lysate (25 ug/Lane) Lane 14: Rat testis tissue lysate (25 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MSH2 Antibody (10G2-R) [NBP3-32605] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32605_mouse-msh2-mab-10g2-r-29820241492619.jpg)
![Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89207_rabbit-monoclonal-4ebp1-antibody-007-23520251154563.png "Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.")
![Western Blot: DEK Antibody [NBP2-98888] - Lane A: HeLa Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:50 kDa](http://images.novusbio.com/fullsize/DEK-Antibody-Western-Blot-NBP2-98888-img0003.jpg "Western Blot: DEK Antibody [NBP2-98888] - Lane A: HeLa Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:50 kDa")
![Immunoprecipitation: DEK Antibody [NBP2-98888] - Lane A:0.5 mg K562 Whole Cell Lysate1 uL anti-DEK rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DEK rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa](http://images.novusbio.com/fullsize/DEK-Antibody-Immunoprecipitation-NBP2-98888-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DEK Antibody [NBP2-98888] - Immunofluorescence staining of DEK in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98888 antibody (dilution ratio 1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98888_rabbit-polyclonal-dek-antibody-23520251449233.png)
![Western Blot: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32638) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32638_rabbit-ndufs2-mab-je65-29-278202415242624.jpg "Western Blot: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32638) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32638) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32638_rabbit-ndufs2-mab-je65-29-298202414115422.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Immunocytochemistry-Immunofluorescence-NBP2-89187-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187] - Analysis of anti-ErbB2 on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified rabbit anti-human ErbB2. Second step staining with FITC conjugated goat anti-rabbit IgG (H+L) polyclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Flow-Cytometry-NBP2-89187-img0003.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187] - Analysis of anti-ErbB2 on SK-BR3 cells. SK-BR3 cells were detached using 1X trypsin, washed, then stained with Purified Rabbit anti-Human ErbB2. Second step staining with FITC conjugated Goat anti-Rabbit IgG polyclonal antibody (BD Pharmingen (R).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Flow-Cytometry-NBP2-89187-img0002.jpg)
![Western Blot: KIF22 Antibody [NBP3-12640] - Lane A: HeLa Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:73 kDaObserved band size:73 kDa](http://images.novusbio.com/fullsize/KIF22-Antibody-Western-Blot-NBP3-12640-img0001.jpg "Western Blot: KIF22 Antibody [NBP3-12640] - Lane A: HeLa Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:73 kDaObserved band size:73 kDa")
![Immunohistochemistry-Paraffin: KIF22 Antibody [NBP3-12640] - Staining of human KIF22 in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/KIF22-Antibody-Immunohistochemistry-Paraffin-NBP3-12640-img0003.jpg)
![Immunohistochemistry-Paraffin: KIF22 Antibody [NBP3-12640] - Staining of human KIF22 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/KIF22-Antibody-Immunohistochemistry-Paraffin-NBP3-12640-img0002.jpg)
![Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-007-Western-Blot-NBP2-89878-img0001.jpg "Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:55 kDa")
![Immunoprecipitation: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-ADSL rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADSL rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-007-Immunoprecipitation-NBP2-89878-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89878 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89878_rabbit-monoclonal-adenylosuccinate-lyase-antibody-007-235202514492358.png)
![Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-298202414262624.jpg "Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-392024848501.jpg)
![Western Blot: SNX5 Antibody (JE64-96) [NBP3-32965] - Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (NBP3-32965) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32965) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32965_rabbit-snx5-mab-je64-96-288202412493454.jpg "Western Blot: SNX5 Antibody (JE64-96) [NBP3-32965] - Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (NBP3-32965) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32965) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNX5 Antibody (JE64-96) [NBP3-32965] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SNX5 antibody (NBP3-32965) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32965) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32965_rabbit-snx5-mab-je64-96-29820241426261.jpg)
![Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (010) [NBP2-90093] - Immunofluorescence staining of Human AK3L1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90093 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90093_rabbit-monoclonal-ak3l1-antibody-010-235202514492370.png "Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (010) [NBP2-90093] - Immunofluorescence staining of Human AK3L1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90093 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Immunoprecipitation: AK3L1 Antibody (010) [NBP2-90093] - AK4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP2-90093 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP2-90093, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa](http://images.novusbio.com/fullsize/nbp2-90093_rabbit-monoclonal-ak3l1-antibody-010-235202516335030.png)
![Western Blot: AK3L1 Antibody (010) [NBP2-90093] - NBP2-90093 at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:26 kDa](http://images.novusbio.com/fullsize/nbp2-90093_rabbit-monoclonal-ak3l1-antibody-010-235202514492360.png)
![Western Blot: CBX3 Antibody [NBP2-98758] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-98758-img0003.jpg "Western Blot: CBX3 Antibody [NBP2-98758] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: CBX3 Antibody [NBP2-98758] - CBX3 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate 2 ul anti-CBX3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CBX3 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NBP2-98758-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-98758] - Immunofluorescence staining of CBX3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98758 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98758_rabbit-polyclonal-cbx3-antibody-23520252135136.png)
![Western Blot: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838] - Western blot analysis of DNA Polymerase delta, catalytic subunit on different lysates with Rabbit anti-DNA Polymerase delta, catalytic subunit antibody (NBP3-32838) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: PC-3M cell lysate (20 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Lane 9: SW480 cell lysate (20 ug/Lane) Predicted band size: 124 kDa Observed band size: 124 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32838) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32838_rabbit-dna-polymerase-delta-catalytic-subunit-mab-je35-73-288202412461636.jpg "Western Blot: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838] - Western blot analysis of DNA Polymerase delta, catalytic subunit on different lysates with Rabbit anti-DNA Polymerase delta, catalytic subunit antibody (NBP3-32838) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: PC-3M cell lysate (20 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Lane 9: SW480 cell lysate (20 ug/Lane) Predicted band size: 124 kDa Observed band size: 124 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32838) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838] - Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-DNA Polymerase delta, catalytic subunit antibody (NBP3-32838) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32838) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32838_rabbit-dna-polymerase-delta-catalytic-subunit-mab-je35-73-298202414115437.jpg)