| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59369-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61719-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22400-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98294-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06434-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12640-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98781-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89878-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98988-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97782-50ul | |||
![Western Blot: Glyoxalase I Antibody (9F11) [NBP2-59369] - Western Blot analysis of Glyoxalase I-BSA Conjugate showing detection of 67 kDa Glyoxalase I protein using Mouse Anti-Glyoxalase I Monoclonal Antibody, Clone 9F11 (NBP2-59369). Lane 1: Molecular Weight Ladder (MW). Lane 2: Glyoxalase I-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Glyoxalase I Monoclonal Antibody (NBP2-59369) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-9F11-Western-Blot-NBP2-59369-img0007.jpg "Western Blot: Glyoxalase I Antibody (9F11) [NBP2-59369] - Western Blot analysis of Glyoxalase I-BSA Conjugate showing detection of 67 kDa Glyoxalase I protein using Mouse Anti-Glyoxalase I Monoclonal Antibody, Clone 9F11 (NBP2-59369). Lane 1: Molecular Weight Ladder (MW). Lane 2: Glyoxalase I-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Glyoxalase I Monoclonal Antibody (NBP2-59369) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (9F11) [NBP2-59369] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-9F11-Immunocytochemistry-Immunofluorescence-NBP2-59369-img0002.jpg)
![Flow Cytometry: Glyoxalase I Antibody (9F11) [NBP2-59369] - Flow Cytometry analysis using Mouse Anti-Glyoxalase I Monoclonal Antibody, Clone 9F11 (NBP2-59369). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Glyoxalase I Monoclonal Antibody (NBP2-59369) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-9F11-Flow-Cytometry-NBP2-59369-img0008.jpg)
![Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Western-Blot-NBP2-61719-img0003.jpg "Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)")
![Flow Cytometry: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis of Hela cells using BUB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Flow-Cytometry-NBP2-61719-img0002.jpg)
![Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against HEK293 (1) and BUB1 (AA: 1-130)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Western-Blot-NBP2-61719-img0004.jpg)
![ELISA: Bub1 Antibody (1A11C7) [NBP2-61719] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-ELISA-NBP2-61719-img0001.jpg)
![Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - analysis of Human Epithelial cell (A431) lysates showing detection of 47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 . Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 47 kDa.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Western-Blot-NBP2-22400-img0001.jpg "Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - analysis of Human Epithelial cell (A431) lysates showing detection of 47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 . Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 47 kDa.")
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Immunocytochemistry-Immunofluorescence-NBP2-22400-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Immunocytochemistry-Immunofluorescence-NBP2-22400-img0003.jpg)
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98294-img0003.jpg "Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-98294] - Immunochemical staining of mouse CRABP2 in mouse kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98294-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody [NBP2-98294] - Immunofluorescence staining of mCRABP2 in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98294 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98294_rabbit-polyclonal-crabp2-antibody-23520251129330.png)
![Western Blot: NUDCD2 Antibody [NBP3-06434] - NUDCD2 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. 4 uL anti-NUDCD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NUDCD2 rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :18 kDa.](http://images.novusbio.com/fullsize/NUDCD2-Antibody-Western-Blot-NBP3-06434-img0003.jpg "Western Blot: NUDCD2 Antibody [NBP3-06434] - NUDCD2 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. 4 uL anti-NUDCD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NUDCD2 rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :18 kDa.")
![Western Blot: NUDCD2 Antibody [NBP3-06434] - Anti-NUDCD2 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:18 kDa.](http://images.novusbio.com/fullsize/NUDCD2-Antibody-Western-Blot-NBP3-06434-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NUDCD2 Antibody [NBP3-06434] - Immunofluorescence staining of NUDCD2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-06434 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06434_rabbit-polyclonal-nudcd2-antibody-235202511444495.png)
![Western Blot: KIF22 Antibody [NBP3-12640] - Lane A: HeLa Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:73 kDaObserved band size:73 kDa](http://images.novusbio.com/fullsize/KIF22-Antibody-Western-Blot-NBP3-12640-img0001.jpg "Western Blot: KIF22 Antibody [NBP3-12640] - Lane A: HeLa Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:73 kDaObserved band size:73 kDa")
![Immunohistochemistry-Paraffin: KIF22 Antibody [NBP3-12640] - Staining of human KIF22 in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/KIF22-Antibody-Immunohistochemistry-Paraffin-NBP3-12640-img0003.jpg)
![Immunohistochemistry-Paraffin: KIF22 Antibody [NBP3-12640] - Staining of human KIF22 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/KIF22-Antibody-Immunohistochemistry-Paraffin-NBP3-12640-img0002.jpg)
![Western Blot: NCAPH Antibody [NBP2-98781] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:82 kDaObserved band size:110 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP2-98781-img0001.jpg "Western Blot: NCAPH Antibody [NBP2-98781] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:82 kDaObserved band size:110 kDa")
![Immunoprecipitation: NCAPH Antibody [NBP2-98781] - Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-NCAPH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NCAPH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 83 kDaObserved band size: 110 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunoprecipitation-NBP2-98781-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP2-98781] - Staining of NCAPH in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-98781 antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98781_rabbit-polyclonal-ncaph-antibody-23520251135415.png)
![Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-007-Western-Blot-NBP2-89878-img0001.jpg "Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:55 kDa")
![Immunoprecipitation: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-ADSL rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADSL rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-007-Immunoprecipitation-NBP2-89878-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89878 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89878_rabbit-monoclonal-adenylosuccinate-lyase-antibody-007-235202514492358.png)
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Western Blot: MSH2 Antibody (10G2-R) [NBP3-32605] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: HEK-293 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: A431 cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: SW480 cell lysate (15 ug/Lane) Lane 7: PC-3M cell lysate (15 ug/Lane) Lane 8: LNCaP cell lysate (negative) (15 ug/Lane) Lane 9: LoVo cell lysate (negative) (15 ug/Lane) Lane 10: NIH/3T3 cell lysate (15 ug/Lane) Lane 11: RAW264.7 cell lysate (15 ug/Lane) Lane 12: PC-12 cell lysate (15 ug/Lane) Lane 13: Mouse testis tissue lysate (25 ug/Lane) Lane 14: Rat testis tissue lysate (25 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32605_mouse-msh2-mab-10g2-r-27820241631687.jpg "Western Blot: MSH2 Antibody (10G2-R) [NBP3-32605] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: HEK-293 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: A431 cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: SW480 cell lysate (15 ug/Lane) Lane 7: PC-3M cell lysate (15 ug/Lane) Lane 8: LNCaP cell lysate (negative) (15 ug/Lane) Lane 9: LoVo cell lysate (negative) (15 ug/Lane) Lane 10: NIH/3T3 cell lysate (15 ug/Lane) Lane 11: RAW264.7 cell lysate (15 ug/Lane) Lane 12: PC-12 cell lysate (15 ug/Lane) Lane 13: Mouse testis tissue lysate (25 ug/Lane) Lane 14: Rat testis tissue lysate (25 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MSH2 Antibody (10G2-R) [NBP3-32605] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32605_mouse-msh2-mab-10g2-r-29820241492619.jpg)
![Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CDCA3-Antibody-Immunohistochemistry-Paraffin-NBP2-98988-img0003.jpg "Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CDCA3-Antibody-Immunohistochemistry-Paraffin-NBP2-98988-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CDCA3 Antibody [NBP2-98988] - Immunofluorescence staining of CDCA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98988 antibody (dilution ratio: 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98988_rabbit-polyclonal-cdca3-antibody-235202521451716.png)
![Western Blot: RPA14 Antibody [NBP2-97782] - Anti-RPA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: HL-60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa](http://images.novusbio.com/fullsize/RPA14-Antibody-Western-Blot-NBP2-97782-img0004.jpg "Western Blot: RPA14 Antibody [NBP2-97782] - Anti-RPA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: HL-60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa")
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP2-97782] - Immunochemical staining of human RPA3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP2-97782-img0003.jpg)
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP2-97782] - Immunochemical staining of human RPA3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP2-97782-img0002.jpg)