| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20098-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20097-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26535-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34643-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50056SS | |||
![Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-2742023924236.png "Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-30C-18 (NBP3-20098, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-2742023924292.png)
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-30C-18 (NBP3-20098) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20098, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-274202396465.png)
![Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (NBP3-20097) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20097, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-2742023924237.png "Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (NBP3-20097) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20097, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Nuclear lysate of MCF7 cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-28B-8 (NBP3-20097) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-274202392428.png)
![Flow Cytometry: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-28B-8 (NBP3-20097, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-274202396466.png)
![Western Blot: PUS1 Antibody [NB100-86983] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86983 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/PUS1-Antibody-Western-Blot-NB100-86983-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PUS1 Antibody [NB100-86983] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86983 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: PUS1 Antibody [NB100-86983] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PUS1 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PUS1-Antibody-Immunohistochemistry-NB100-86983-img0007.jpg)
![Western Blot: SMARCA6 Antibody [NB100-278] - Detection of Human HELLS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and K562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HELLS antibody NB100-278 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMARCA6-Antibody-Western-Blot-NB100-278-img0004.jpg "Western Blot: SMARCA6 Antibody [NB100-278] - Detection of Human HELLS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and K562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HELLS antibody NB100-278 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: NOL1 Antibody [NBP1-18907] - Detection of Human NOL1 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-NOL1 antibody NBP1-18907 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NOL1-Antibody-Western-Blot-NBP1-18907-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: NOL1 Antibody [NBP1-18907] - Detection of Human NOL1 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-NOL1 antibody NBP1-18907 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: PPP4C Antibody [NBP2-19912] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/PPP4C-Antibody-Western-Blot-NBP2-19912-img0002.jpg "Western Blot: PPP4C Antibody [NBP2-19912] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: PPP4C Antibody [NBP2-19912] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PPP4C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19912-img0003.jpg)
![Western Blot: PPP4C Antibody [NBP2-19912] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/PPP4C-Antibody-Western-Blot-NBP2-19912-img0001.jpg)
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-2622024151064.jpg "Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.")
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-272202485630.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Staining of A549 with HIST1H3A [ac Lys18] Antibody (30A7) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 490-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202412493638.jpg)
![Flow Cytometry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Overlay Peak curve showing A549 cells stained with HIST1H3A [ac Lys18] Antibody (30A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202414312785.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Immunocytochemistry-Immunofluorescence-NBP2-32864-img0005.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).")
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Flow Cytometric Analysis of human MCF-7 cells. ErbB2/Her2 Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Flow-Cytometry-NBP2-32864-img0003.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/718) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Protein-Array-NBP2-32864-img0004.jpg)
![Western Blot: PRMT3 Antibody [NBP2-19933] - PRMT3 antibody immunoprecipitates PRMT3 protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of PRMT3 protein by 4 µg PRMT3 antibody. 7.5 % SDS-PAGE. The immunoprecipitated PRMT3 protein was detected by PRMT3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PRMT3-Antibody-Western-Blot-NBP2-19933-img0004.jpg "Western Blot: PRMT3 Antibody [NBP2-19933] - PRMT3 antibody immunoprecipitates PRMT3 protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of PRMT3 protein by 4 µg PRMT3 antibody. 7.5 % SDS-PAGE. The immunoprecipitated PRMT3 protein was detected by PRMT3 antibody diluted at 1:500.")
![Western Blot: PRMT3 Antibody [NBP2-19933] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PRMT3-Antibody-Western-Blot-NBP2-19933-img0001.jpg)
![Western Blot: PRMT3 Antibody [NBP2-19933] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PRMT3-Antibody-Western-Blot-NBP2-19933-img0002.jpg)
![Western Blot: PRMT3 Antibody [NBP2-19933] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT3 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRMT3-Antibody-Western-Blot-NBP2-19933-img0003.jpg)
![Western Blot: Aldolase C Antibody (E9) [NBP2-50056] - Right: Blots of rat brain lysate blotted with NBP2-50056. NBP2-50056 binds strongly and cleanly to a band at about 40 kDa. This antibody binds to an epitope shared by the closely related Aldolase A, B and C, recognizing all three on western blots. Above: Blots of recombinant human Aldolase A, B and C with NBP2-50056 as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged human Aldolase A, B and C respectively. The epitope for NBP2-50056 is within the core of the three Aldolase molecules which are less conserved than the N and C-termini where Aldolase C specific antibodies bind. The specificity of two other Aldolase antibodies is shown in the indicated parts of the image. One antibody binds to the N-terminal peptide (clone 4A9) and one to the C-terminal peptide (clone 1A1).](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-E9-Western-Blot-NBP2-50056-img0004.jpg "Western Blot: Aldolase C Antibody (E9) [NBP2-50056] - Right: Blots of rat brain lysate blotted with NBP2-50056. NBP2-50056 binds strongly and cleanly to a band at about 40 kDa. This antibody binds to an epitope shared by the closely related Aldolase A, B and C, recognizing all three on western blots. Above: Blots of recombinant human Aldolase A, B and C with NBP2-50056 as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged human Aldolase A, B and C respectively. The epitope for NBP2-50056 is within the core of the three Aldolase molecules which are less conserved than the N and C-termini where Aldolase C specific antibodies bind. The specificity of two other Aldolase antibodies is shown in the indicated parts of the image. One antibody binds to the N-terminal peptide (clone 4A9) and one to the C-terminal peptide (clone 1A1).")
![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (E9) [NBP2-50056] - View of mixed neuron/glial cultures stained with NBP2-50056 (red) and chicken antibody to GFAP (NBP1-05198, green). NBP2-50056 reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Aldolase stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-E9-Immunocytochemistry-Immunofluorescence-NBP2-50056-img0003.jpg)
![Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 Antibody (186) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-186-Western-Blot-NBP2-43701-img0005.jpg "Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 Antibody (186) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Analysis of A. 30 ug A549 whole cell lysate/extract B. 30 ug H1299 whole cell lysate/extract C. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [186] dilution: 1:10000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-186-Western-Blot-NBP2-43701-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [186].](http://images.novusbio.com/fullsize/Enolase-1-Antibody-186-Western-Blot-NBP2-43701-img0003.jpg)
![Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 Antibody (186) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-186-Western-Blot-NBP2-43701-img0004.jpg)
![Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - HeLa whole cell extract.](http://images.novusbio.com/fullsize/DNA-Polymerase-delta-catalytic-subunit-Antibody-607-Western-Blot-NB100-476-img0003.jpg "Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - HeLa whole cell extract.")
![Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DNA-Polymerase-delta-catalytic-subunit-Antibody-607-Western-Blot-NB100-476-img0004.jpg)
![Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA Polymerase delta, catalytic subunit antibody [607] (NB100-476) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-476_mouse-monoclonal-dna-polymerase-delta-catalytic-subunit-antibody-607-91020242075934.jpg)