| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55731-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25991 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26000 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26003 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12599-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20135-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody [NBP2-55731] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55731-img0001.jpg "Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody [NBP2-55731] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Immunofluorescence-NBP2-59302-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - ChIP assays were performed using human HeLa cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the EIF4A2 and GAPDH genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Chromatin-Immunoprecipitation-NBP2-59302-img0002.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K27ac. The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-ELISA-NBP2-59302-img0005.jpg)
![Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Western-Blot-NBP2-59210-img0001.jpg "Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59210-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K79me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,500.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-ELISA-NBP2-59210-img0002.jpg)
![Western Blot: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61531 at 1 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys79-Antibody-RM147-Western-Blot-NBP2-61531-img0006.jpg "Western Blot: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61531 at 1 ug/ml. Observed molecular weight is ~17 kDa.")
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys79] Antibody (RM147) - Azide and BSA Free [NBP2-61531] - RM147 specifically reacts to Histone H3 monomethylated at Lysine 79. No cross reactivity with dimethylated Lysine 79 or trimethylated Lysine 79, or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp2-61531_rabbit-monoclonal-histone-h3-monomethyl-lys79-antibody-rm147-multiplex-immunoassay-189202523512117.png)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - ChIP performed on HeLa cells using H3K79me1 antibody (NBP2-61531). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61531_rabbit-monoclonal-histone-h3-monomethyl-lys79-antibody-rm147-chromatin-immunoprecipitation-189202523512123.png)
![Western Blot: Histone H3 [ac Lys4] Antibody (RM149) [NBP2-61542] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61542, showed a band of Histone H3 acetylated at Lysine 4 in treated HeLa. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys4-Antibody-RM149-Western-Blot-NBP2-61542-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys4] Antibody (RM149) [NBP2-61542] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61542, showed a band of Histone H3 acetylated at Lysine 4 in treated HeLa. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP2-61542] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM149 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61542_rabbit-monoclonal-histone-h3-ac-lys4-antibody-rm149-immunocytochemistry-immunofluorescence-18920252337401.png)
![Dot Blot: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - A Peptide dot blot shows NBP2-61514 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys18-Antibody-RM168-Dot-Blot-NBP2-61514-img0006.jpg "Dot Blot: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - A Peptide dot blot shows NBP2-61514 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).")
![Western Blot: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61514 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys18-Antibody-RM168-Western-Blot-NBP2-61514-img0004.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys18] Antibody (RM168) - Azide and BSA Free [NBP2-61514] - RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61514_rabbit-monoclonal-histone-h3-dimethyl-lys18-antibody-rm168-multiplex-immunoassay-18920252337119.png)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0003.jpg "Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Histone H3 (phospho Thr3) antibody detects Histone H3 (phospho Thr3) protein by western blot analysis. Un-treated (-) and treated (+, 0.1uM Calyculin for 30 minutes + phosphatase inhibitor) HeLa whole cell extracts (2 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0001.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0002.jpg)
![SDS-Page: Recombinant Human p62/SQSTM1 Protein [NBP1-44490] - p62/SQSTM1 Protein SQSTM1, 39.7 kDa (364aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/Recombinant-Human-p62-SQSTM1-Protein-SDS-Page-NBP1-44490-img0003.jpg "SDS-Page: Recombinant Human p62/SQSTM1 Protein [NBP1-44490] - p62/SQSTM1 Protein SQSTM1, 39.7 kDa (364aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE")
![Western Blot: SmarcAL1 Antibody [NBP3-12599] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:106 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/SmarcAL1-Antibody-Western-Blot-NBP3-12599-img0001.jpg "Western Blot: SmarcAL1 Antibody [NBP3-12599] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:106 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: SmarcAL1 Antibody [NBP3-12599] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SMARCAL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SMARCAL1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 106 kDaObserved band size :120 kDa](http://images.novusbio.com/fullsize/SmarcAL1-Antibody-Immunoprecipitation-NBP3-12599-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SmarcAL1 Antibody [NBP3-12599] - Staining of SMARCAL1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12599 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-12599_rabbit-polyclonal-smarcal1-antibody-23520251633501.png)
![Flow Cytometry: Cbx2 Antibody (RAB-C238) - Chimeric - Azide and BSA Free [NBP3-20135] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C238 (NBP3-20135, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20135_rabbit-cbx2-mab-rab-c238-chimeric-azide-and-bsa-free-2742023924270.png "Flow Cytometry: Cbx2 Antibody (RAB-C238) - Chimeric - Azide and BSA Free [NBP3-20135] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C238 (NBP3-20135, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Cbx2 Antibody (RAB-C238) - Chimeric - Azide and BSA Free [NBP3-20135] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C238 (NBP3-20135) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20135, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20135_rabbit-cbx2-mab-rab-c238-chimeric-azide-and-bsa-free-2742023924222.png)
![Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 WDR5 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/WDR5-Antibody-Immunohistochemistry-NBP1-30063-img0004.jpg "Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 WDR5 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: WDR5 Antibody [NBP1-30063] - Detection of human WDR5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody A302-429A (lot A302-429A-2) used for IP at 6 ug per reaction. WDR5 was also immunoprecipitated by rabbit anti-WDR5 antibody A302-430A. For blotting immunoprecipitated WDR5, A302-430A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/WDR5-Antibody-Immunoprecipitation-NBP1-30063-img0006.jpg)