| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87696-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83030-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33763-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12073-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-138SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26172-50ul | |||
![Western Blot: BMAL1 Antibody [NBP2-15599] - Analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody. A: Non-transfected lysate. B: ARNTL transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NBP2-15599-img0005.jpg "Western Blot: BMAL1 Antibody [NBP2-15599] - Analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody. A: Non-transfected lysate. B: ARNTL transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody [NBP2-15599] - A375 cells. Green: MOP3 protein stained by MOP3 antibody [N1N3] Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15599-img0003.jpg)
![Western Blot: BMAL1 Antibody [NBP2-15599] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NBP2-15599-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BMAL1 Antibody [NBP2-15599] - Treated 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] and PER2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15599_rabbit-polyclonal-bmal1-antibody-1610202419405778.jpg)
![Western Blot: FABP2/I-FABP Antibody [NBP1-87696] - Analysis in control (vector only transfected HEK293T lysate) and FABP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-Western-Blot-NBP1-87696-img0006.jpg "Western Blot: FABP2/I-FABP Antibody [NBP1-87696] - Analysis in control (vector only transfected HEK293T lysate) and FABP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NBP1-87696] - Staining in human small intestine and liver tissues using anti-FABP2 antibody. Corresponding FABP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87696-img0005.jpg)
![Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NBP1-87696] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87696-img0002.jpg)
![Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NBP1-87696] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87696-img0004.jpg)
![Western Blot: Lipin 1 Antibody [NBP1-83030] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Lipin-1-Antibody-Western-Blot-NBP1-83030-img0006.jpg "Western Blot: Lipin 1 Antibody [NBP1-83030] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Lipin 1 Antibody [NBP1-83030] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.](http://images.novusbio.com/fullsize/Lipin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83030-img0009.jpg)
![Immunohistochemistry-Paraffin: Lipin 1 Antibody [NBP1-83030] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Lipin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-83030-img0008.jpg)
![Immunohistochemistry-Paraffin: FABP3/H-FABP Antibody [NBP2-33763] - Staining in human heart muscle and duodenum tissues using anti-FABP3 antibody. Corresponding FABP3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP3-H-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-33763-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP3/H-FABP Antibody [NBP2-33763] - Staining in human heart muscle and duodenum tissues using anti-FABP3 antibody. Corresponding FABP3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FABP3/H-FABP Antibody [NBP2-33763] - Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/FABP3-H-FABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33763-img0002.jpg)
![Immunohistochemistry-Paraffin: FABP3/H-FABP Antibody [NBP2-33763] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/fullsize/FABP3-H-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-33763-img0004.jpg)
![Immunohistochemistry-Paraffin: FABP3/H-FABP Antibody [NBP2-33763] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/FABP3-H-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-33763-img0005.jpg)
![Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] Antibody [NBP3-05459] - Analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S660 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (10 uM) at 37c for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Hormone-sensitive-Lipase-HSL-[p-Ser660]-Antibody-Western-Blot-NBP3-05459-img0001.jpg "Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] Antibody [NBP3-05459] - Analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S660 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (10 uM) at 37c for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - HeLa whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-GT25212-Western-Blot-NBP3-13609-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - HeLa whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - Wild-type (WT) and LAMP-1/CD107a knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-GT25212-Western-Blot-NBP3-13609-img0005.jpg)
![LAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: LAMP1 stained by LAMP1 antibody [GT25212] (NBP3-13609) diluted at 1:2000.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13609_mouse-lamp-1-cd107a-mab-gt25212-immunocytochemistry-immunofluorescence-261120251839579.jpg)
![HeLa whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (NBP3-13609) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13609_mouse-lamp-1-cd107a-mab-gt25212-western-blot-2611202518473215.jpg)
![Western Blot: Apolipoprotein B/ApoB Antibody (6G6) [NBP2-37598] - Analysis using ApoB mAb against human ApoB (AA: 3900-4110) recombinant protein. (Expected MW is 45 kDa)](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-6G6-Western-Blot-NBP2-37598-img0002.jpg "Western Blot: Apolipoprotein B/ApoB Antibody (6G6) [NBP2-37598] - Analysis using ApoB mAb against human ApoB (AA: 3900-4110) recombinant protein. (Expected MW is 45 kDa)")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein B/ApoB Antibody (6G6) [NBP2-37598] - Analysis of HepG2 cells using ApoB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-6G6-Immunocytochemistry-Immunofluorescence-NBP2-37598-img0003.jpg)
![Flow Cytometry: Apolipoprotein B/ApoB Antibody (6G6) [NBP2-37598] - Analysis of PBMC using ApoB mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-6G6-Flow-Cytometry-NBP2-37598-img0004.jpg)
![ELISA: Apolipoprotein B/ApoB Antibody (6G6) [NBP2-37598] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-6G6-ELISA-NBP2-37598-img0001.jpg)
![Western Blot: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32977) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-288202412493476.jpg "Western Blot: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32977) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32977) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-2982024149263.jpg)
![Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Immunocytochemistry analysis of HeLa cells labeling SREBP1 with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-29202410484870.jpg)
![Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Western-Blot-NBP2-67191-img0002.jpg "Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0001.jpg)
![Western Blot: SR-BI Antibody (185-1G2 (B467)) - Chimeric [NBP3-12073] - Human peripheral blood monocyte cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 185-1G2 (B467) (NBP3-12073) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/SR-BI-Antibody-185-1G2-B467-Chimeric-Western-Blot-NBP3-12073-img0001.jpg "Western Blot: SR-BI Antibody (185-1G2 (B467)) - Chimeric [NBP3-12073] - Human peripheral blood monocyte cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 185-1G2 (B467) (NBP3-12073) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: SR-BI Antibody (185-1G2 (B467)) - Chimeric - Azide and BSA Free [NBP3-12073] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips stained with NBP3-12073 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12073, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12073_rabbit-monoclonal-sr-bi-antibody-185-1g2-b467-chimeric-74202522141242.png)
![Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] - Image of LDLR Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26172_rabbit-ldlr-mab-2b10-262202415475620.jpg "Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] - Image of LDLR Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] - Image of LDLR Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26172_rabbit-ldlr-mab-2b10-27220248313719.jpg)
![Immunocytochemistry/Immunofluorescence: LDLR Antibody (2B10) [NBP3-26172] - Staining of Hela Cells with LDLR Antibody (2B10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26172_rabbit-ldlr-mab-2b10-282202412493641.jpg)
![Flow Cytometry: LDLR Antibody (2B10) [NBP3-26172] - Overlay histogram showing Hela cells stained with LDLR Antibody (2B10) (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26172_rabbit-ldlr-mab-2b10-282202414183657.jpg)