| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-173SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34563-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26386-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26494-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22394-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27721-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21602-50ul | |||
![Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500](http://images.novusbio.com/fullsize/Rad17-Antibody-Western-Blot-NB100-173-img0001.jpg "Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500")
![Western Blot: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10ug/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1ug/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32731) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-27820241631671.jpg "Western Blot: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10ug/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1ug/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32731) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32731) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-29820241433368.jpg)
![Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-288202412442975.jpg "Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-29202410234274.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - IHC-Prediluted [NBP2-44980] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-+-DO-7-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44980-img0001.jpg "Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - IHC-Prediluted [NBP2-44980] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)")
![Western Blot: Chk1 Antibody [NB100-274] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-274-img0133.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-274] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-274] - Whole cell lysate (30 ug) from HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-274-img0132.jpg)
![Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate). All lanes: p53 [p Thr55] Antibody at 1.28ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 KDa Observed band size: 53 KDa](http://images.novusbio.com/fullsize/nbp3-26386_rabbit-p53-p-thr-55-mab-3c3-282202411351620.jpg "Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate). All lanes: p53 [p Thr55] Antibody at 1.28ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 KDa Observed band size: 53 KDa")
![Immunocytochemistry/Immunofluorescence: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Staining of 293 cells (treated with 50mM Calyculin A for 30min) with p53 [p Thr55] Antibody (3C3) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26386_rabbit-p53-p-thr-55-mab-3c3-282202412493626.jpg)
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-262202415355749.jpg "Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-27220248313715.jpg)
![Flow Cytometry: ATM Antibody (3G11) [NBP3-26494] - Overlay histogram showing Hela cells stained with ATM Antibody (3G11) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-282202414312732.jpg)
![Western Blot: ATRIP Antibody (SR2262) [NBP3-22394] - Western blot analysis using NBP3-22394 in PC-3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22394_rabbit-atrip-mab-sr2262-57202317215154.jpg "Western Blot: ATRIP Antibody (SR2262) [NBP3-22394] - Western blot analysis using NBP3-22394 in PC-3 cell lysate.")
![Western Blot: MLH1 Antibody (S07-5D3) [NBP3-14898] - Western blot detection of MLH1 in Hela, A549, HL-60, U2OS cell lysates using MLH1 Rabbit mAb (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/MLH1-Antibody-S07-5D3-Western-Blot-NBP3-14898-img0001.jpg "Western Blot: MLH1 Antibody (S07-5D3) [NBP3-14898] - Western blot detection of MLH1 in Hela, A549, HL-60, U2OS cell lysates using MLH1 Rabbit mAb (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (S07-5D3) [NBP3-14898] - Immunocytochemistry of MLH1 (green) in hela using NBP3-14898 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14898_rabbit-mlh1-mab-s07-5d3-113202515145417.jpg)
![Western Blot: p53 [ac Lys 382] Antibody (SR2907) [NBP3-27721] - Western blot analysis of Jurkat cell lysate treated with etopside and TSA.](http://images.novusbio.com/fullsize/nbp3-27721_rabbit-p53-ac-lys-382-mab-sr2907-75202410133475.jpg "Western Blot: p53 [ac Lys 382] Antibody (SR2907) [NBP3-27721] - Western blot analysis of Jurkat cell lysate treated with etopside and TSA.")
![Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot detection of Chk1 in K562 cell lysates using Chk1 Rabbit mAb (1:1000 diluted). Predicted band size: 54KDa. Observed band size: 54KDa.](http://images.novusbio.com/fullsize/Chk1-Antibody-S07-4K2-Western-Blot-NBP3-14889-img0001.jpg "Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot detection of Chk1 in K562 cell lysates using Chk1 Rabbit mAb (1:1000 diluted). Predicted band size: 54KDa. Observed band size: 54KDa.")
![Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot of K562, 3T3, Hela cell lysates using NBP3-14889. Predicted band size: 54kDa. Observed band size: 54kDa.](http://images.novusbio.com/fullsize/nbp3-14889_rabbit-chk1-mab-s07-4k2-103202523464531.jpg)
![Western Blot: Rad17 [p Ser 656] Antibody (SR2259) [NBP3-21602] - Western blot analysis using NBP3-21602 in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21602_rabbit-rad17-p-ser-656-mab-sr2259-67202310620.jpg "Western Blot: Rad17 [p Ser 656] Antibody (SR2259) [NBP3-21602] - Western blot analysis using NBP3-21602 in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate.")