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![Western Blot: p53 Antibody (S07-6A7) [NBP3-20045] - Western blot detection of p53 in Jurkat cell lysates using NBP3-20045 (1:500 diluted). Predicted band size: 44kDa. Observed band size: 53kDa.](http://images.novusbio.com/fullsize/p53-Antibody-S07-6A7-Western-Blot-NBP3-20045-img0002.jpg "Western Blot: p53 Antibody (S07-6A7) [NBP3-20045] - Western blot detection of p53 in Jurkat cell lysates using NBP3-20045 (1:500 diluted). Predicted band size: 44kDa. Observed band size: 53kDa.")
![Immunocytochemistry/Immunofluorescence: p53 Antibody (S07-6A7) [NBP3-20045] - Immunofluorescence of p53 (green) in MCF-7 using NBP3-20045 at dilution 1/20, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-20045_rabbit-p53-mab-s07-6a7-113202515145466.jpg)
![SDS-Page: Recombinant Human Mre11 Protein [H00004361-P01] - Analysis of with a 12.5% SDS-PAGE gel stained with Coomassie Blue. A band appears at the theoretical molecular weight of 48.4 kDa.](http://images.novusbio.com/fullsize/qc_test-H00004361-P01-1.jpg "SDS-Page: Recombinant Human Mre11 Protein [H00004361-P01] - Analysis of with a 12.5% SDS-PAGE gel stained with Coomassie Blue. A band appears at the theoretical molecular weight of 48.4 kDa.")
![Western Blot: Choline kinase alpha Antibody [H00001119-D01P] - Analysis of CHKA expression in transfected 293T cell line by CHKA polyclonal antibody.Lane 1: CHKA transfected lysate(52.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Choline-kinase-alpha-Antibody-Western-Blot-H00001119-D01P-img0002.jpg "Western Blot: Choline kinase alpha Antibody [H00001119-D01P] - Analysis of CHKA expression in transfected 293T cell line by CHKA polyclonal antibody.Lane 1: CHKA transfected lysate(52.20 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Choline kinase alpha Antibody [H00001119-D01P] - Analysis of antibody to CHKA on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/Choline-kinase-alpha-Antibody-Immunocytochemistry-Immunofluorescence-H00001119-D01P-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ATR Antibody (3F2) [H00000545-M01] - Analysis of monoclonal antibody to ATR on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ATR-Antibody-3F2-Immunocytochemistry-Immunofluorescence-H00000545-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: ATR Antibody (3F2) [H00000545-M01] - Analysis of monoclonal antibody to ATR on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: ATR Antibody (3F2) [H00000545-M01] - Detection limit for recombinant GST tagged ATR is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATR-Antibody-3F2-ELISA-H00000545-M01-img0006.jpg)
![Western Blot: Wee1 Antibody [H00007465-B01P] - Analysis of WEE1 expression in transfected 293T cell line by WEE1 polyclonal antibody. Lane 1: WEE1 transfected lysate(71.06 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Wee1-Antibody-Western-Blot-H00007465-B01P-img0002.jpg "Western Blot: Wee1 Antibody [H00007465-B01P] - Analysis of WEE1 expression in transfected 293T cell line by WEE1 polyclonal antibody. Lane 1: WEE1 transfected lysate(71.06 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Wee1 Antibody [H00007465-B01P] - Analysis of purified antibody to WEE1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Wee1-Antibody-Immunocytochemistry-Immunofluorescence-H00007465-B01P-img0001.jpg)
![Western Blot: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of CHKB expression in transfected 293T cell line by CHKB polyclonal antibody. Lane 1: CHKB transfected lysate(43.45 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Choline-Kinase-beta-Antibody-Western-Blot-H00001120-B01P-img0004.jpg "Western Blot: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of CHKB expression in transfected 293T cell line by CHKB polyclonal antibody. Lane 1: CHKB transfected lysate(43.45 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of purified antibody to CHKB on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Choline-Kinase-beta-Antibody-Immunocytochemistry-Immunofluorescence-H00001120-B01P-img0003.jpg)
![Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01] - Analysis of protein-protein interactions between CCNB1 and CDC25A. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-CDC25A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CDC25A-Antibody-3D5-Proximity-Ligation-Assay-H00000993-M01-img0002.jpg "Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01] - Analysis of protein-protein interactions between CCNB1 and CDC25A. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-CDC25A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: CDC25A Antibody (3D5) [H00000993-M01] - Detection limit for recombinant GST tagged CDC25A is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC25A-Antibody-3D5-Sandwich-ELISA-H00000993-M01-img0003.jpg)
![Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-288202412442975.jpg "Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-29202410234274.jpg)
![Immunohistochemistry: p53 DINP1 Antibody - BSA Free [NBP3-45755] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (p53 DINP1 antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-45755_rabbit-p53-dinp1-pab-bsa-free-284202512582617.jpg "Immunohistochemistry: p53 DINP1 Antibody - BSA Free [NBP3-45755] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (p53 DINP1 antibody) at dilution of 1:50")
![Western Blot: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10ug/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1ug/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32731) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-27820241631671.jpg "Western Blot: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10ug/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1ug/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32731) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32731) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-29820241433368.jpg)
