| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81094-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12078-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50162-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12057-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52658-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09043-0.025mg | |||
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (10F6B12A9) - BSA Free [NBP3-43566] - Immunofluorescence analysis of paraformaldehyde-fixed Caco-2 cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-43566 (1:100 dilution) for 1 hour followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left to right, top to bottom, NBP3-43566, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43566_mouse-cytokeratin-20-mab-10f6b12a9-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2220260325816.png "Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (10F6B12A9) - BSA Free [NBP3-43566] - Immunofluorescence analysis of paraformaldehyde-fixed Caco-2 cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-43566 (1:100 dilution) for 1 hour followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left to right, top to bottom, NBP3-43566, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.")
![Western Blot: Cytokeratin 20 Antibody (10F6B12A9) - BSA Free [NBP3-43566] - Caco-2 (A) cells lysate and human colon (B) tissue lysate (35µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43566 at 1µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43566_mouse-cytokeratin-20-mab-10f6b12a9-azide-and-bsa-free-western-blot-222026032583.png)
![Immunocytochemistry/Immunofluorescence: Transferrin Antibody (TW4.20) [NBP2-81094] - Immunofluorescence staining of fixed HepG2 cells with Transferrin Antibody (TW4.20) [NBP2-81094]. Immunofluorescence analysis of paraformaldehyde fixed HpeG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of TW4.20 [NBP2-81094] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing diffuse cytoplasmic staining, likely from protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81094, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Transferrin-Antibody-TW4-20-Immunocytochemistry-Immunofluorescence-NBP2-81094-img0001.jpg "Immunocytochemistry/Immunofluorescence: Transferrin Antibody (TW4.20) [NBP2-81094] - Immunofluorescence staining of fixed HepG2 cells with Transferrin Antibody (TW4.20) [NBP2-81094]. Immunofluorescence analysis of paraformaldehyde fixed HpeG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of TW4.20 [NBP2-81094] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing diffuse cytoplasmic staining, likely from protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81094, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: CD59 Antibody (193-27) - Chimeric [NBP3-12078] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 193-27 (NBP3-12078) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12078, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD59-Antibody-193-27-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12078-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD59 Antibody (193-27) - Chimeric [NBP3-12078] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 193-27 (NBP3-12078) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12078, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078] - Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12078 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12078_rabbit-monoclonal-cd59-antibody-193-27-chimeric-74202522141239.png)
![In vitro assay: Recombinant Rat alpha-Synuclein Active, Monomer Protein [NBP3-14773] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer (NBP3-14773) is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril is the majority of the seed.](http://images.novusbio.com/fullsize/Recombinant-Rat-alpha-Synuclein-Active--Monomer-Protein-In-vitro-assay-NBP3-14773-img0001.jpg "In vitro assay: Recombinant Rat alpha-Synuclein Active, Monomer Protein [NBP3-14773] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer (NBP3-14773) is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril is the majority of the seed.")
![SDS-Page: Recombinant Rat alpha-Synuclein Active, Monomer Protein [NBP3-14773] - SDS-PAGE of Rat alpha-Synuclein Protein Monomer (NBP3-14773)](http://images.novusbio.com/fullsize/Recombinant Rat alpha-Synuclein Active- Monomer Protein-SDS-Page-NBP3-14773-img0002.jpg)
![Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NBP3-11044-img0001.jpg "Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.")
![Dot Blot: Tau Antibody [NBP3-11044] - Dot blot analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.](http://images.novusbio.com/fullsize/Tau-Antibody-Dot-Blot-NBP3-11044-img0002.jpg)
![Flow Cytometry: CD4 Antibody (YNB46.1.8 (Campath-9H)) - Chimeric [NBP2-52658] - Flow-cytometry using anti-CD4 antibody YNB46.1.8 (Campath-9H) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of YNB46.1.8/Campath-9H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-YNB46-1-8-Campath-9H-Chimeric-Flow-Cytometry-NBP2-52658-img0002.jpg "Flow Cytometry: CD4 Antibody (YNB46.1.8 (Campath-9H)) - Chimeric [NBP2-52658] - Flow-cytometry using anti-CD4 antibody YNB46.1.8 (Campath-9H) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of YNB46.1.8/Campath-9H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Western Blot: PYK2/FAK2 Antibody (M229) [NBP3-23221] - Western blot analysis of mouse brain. The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23221_mouse-pyk2-fak2-mab-m229-199202312403720.jpg "Western Blot: PYK2/FAK2 Antibody (M229) [NBP3-23221] - Western blot analysis of mouse brain. The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).")
![Western Blot: beta-Actin Antibody (M008) [NBP3-23324] - Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-beta-Actin (NBP3-23324) at 1:1000 (lanes 1-6).](http://images.novusbio.com/fullsize/nbp3-23324_mouse-beta-actin-mab-m008-19920231820297.jpg "Western Blot: beta-Actin Antibody (M008) [NBP3-23324] - Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-beta-Actin (NBP3-23324) at 1:1000 (lanes 1-6).")
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (M008) [NBP3-23324] - Immunocytochemical labeling of beta-Actin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-beta-Actin (clone M008). The antibody was detected using goat anti-mouse DyLight (R) 594.](http://images.novusbio.com/fullsize/nbp3-23324_mouse-beta-actin-mab-m008-1992023141019.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric [NBP3-09043] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of MHM24 (NBP3-09043) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing diffuse membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09043, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-L-CD11a-Antibody-MHM24-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09043-img0002.jpg "Immunocytochemistry/Immunofluorescence: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric [NBP3-09043] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of MHM24 (NBP3-09043) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing diffuse membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09043, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric [NBP3-09043] - Jurkat cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of MHM24 (NBP3-09043, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-alpha-L-CD11a-Antibody-MHM24-Chimeric-Flow-Cytometry-NBP3-09043-img0001.jpg)
![Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.](http://images.novusbio.com/fullsize/nbp3-23342_rabbit-profilin-1-p-ser138-pab-18920231735520.jpg "Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.")
![Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of human recombinant Profilin-1 phosphorylated in vitro with PKC alpha kinase then untreated (-) or treated with lambdaphosphatase (+). The blots were probed with anti-Profilin (a.a. 126-137) (left panel) or anti-Profilin (Ser-138) phospho-specific (right panel) antibodies at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23342_rabbit-profilin-1-p-ser138-pab-199202312523637.jpg)