| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50222-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12014-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52651-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48188 | |||
| Azide and BSA Free | NBP2-34399-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34567-0.1mg | |||
![SDS-Page: Recombinant Human Vinculin Protein [H00007414-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007414-P01-1.jpg "SDS-Page: Recombinant Human Vinculin Protein [H00007414-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PK110) [NBP2-50222] - HaCaTs (keratinocytes) were stained with Cytokeratin, pan antibody (PK110) [DyLight 488] (NBP2-50222G). Cells were fixed with PFA 4%. Antibody concentration: 1:100. Dilution buffer: 1X PBS, 1% bovine serum albumin (BSA). Image from verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PK110-Immunocytochemistry-Immunofluorescence-NBP2-50222-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PK110) [NBP2-50222] - HaCaTs (keratinocytes) were stained with Cytokeratin, pan antibody (PK110) [DyLight 488] (NBP2-50222G). Cells were fixed with PFA 4%. Antibody concentration: 1:100. Dilution buffer: 1X PBS, 1% bovine serum albumin (BSA). Image from verified customer review.")
![Immunohistochemistry-Frozen: Cytokeratin, pan Antibody (PK110) [NBP2-50222] - Cytokeratin was detected in human skin using Cytokeratin, pan antibody (PK110) [DyLight 488] (NBP2-50222G) (1:100; 1 hour) in PBS. Tissue was fixed in acetone. Image from verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PK110-Immunohistochemistry-Frozen-NBP2-50222-img0001.jpg)
![Western Blot: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric [NBP3-12014] - HeLa(A) (0.00001ug/ml), HepG2(B) (0.0003ug/ml) and A549(C) (0.00001ug/ml) cell lysate and human thyroid(D) (0.0003ug/ml) and human placenta(E) (0.00001ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 (NBP3-12014) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL-12-30-Chimeric-Western-Blot-NBP3-12014-img0001.jpg "Western Blot: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric [NBP3-12014] - HeLa(A) (0.00001ug/ml), HepG2(B) (0.0003ug/ml) and A549(C) (0.00001ug/ml) cell lysate and human thyroid(D) (0.0003ug/ml) and human placenta(E) (0.00001ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 (NBP3-12014) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric - Azide and BSA Free [NBP3-12014] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12014 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12014_rabbit-monoclonal-cytokeratin-7-antibody-ov-tl-12-30-chimeric-74202522113044.png)
![Immunocytochemistry /Immunofluorescence: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric - Azide and BSA Free [NBP3-12014] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with NBP3-12014 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-12014, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12014_rabbit-monoclonal-cytokeratin-7-antibody-ov-tl-12-30-chimeric-74202522113016.png)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52651_rabbit-monoclonal-egfr-antibody-528-chimeric-74202522141227.png "Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers . A much greater increase in fluorescence is seen when 100 uM monomer is combined with 10 uM of fibrils (NBP3-14767) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-In-vitro-assay-NBP3-14767-img0001.jpg "In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers . A much greater increase in fluorescence is seen when 100 uM monomer is combined with 10 uM of fibrils (NBP3-14767) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.")
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - TEM of A53T alpha synuclein Pre-formed Fibrils (NBP3-14767)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-Electron-Microscopy-NBP3-14767-img0003.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - TEM of A53T alpha synuclein Pre-formed Fibrils (NBP3-14767)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-Electron-Microscopy-NBP3-14767-img0002.jpg)
![In vitro assay: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril (NBP3-14774) is the majority of the seed.](http://images.novusbio.com/fullsize/Recombinant-Rat-alpha-Synuclein-Active--Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14774-img0001.jpg "In vitro assay: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril (NBP3-14774) is the majority of the seed.")
![Electron Microscopy: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - TEM of Rat alpha-Synuclein Pre-formed Fibrils (PFFS) (NBP3-14774). 500nm](http://images.novusbio.com/fullsize/Recombinant Rat alpha-Synuclein Active- Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14774-img0003.jpg)
![Electron Microscopy: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - TEM of Rat alpha-Synuclein Pre-formed Fibrils (PFFS) (NBP3-14774). 200nm](http://images.novusbio.com/fullsize/Recombinant-Rat-alpha-Synuclein-Active--Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14774-img0002.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Acetyl (N-Term) Protein [NBP3-14769] - Thioflavin T assay for N-terminal acetylated alpha synuclein monomers and pre-formed fibrils (PFFs) (NBP3-14769). Increased fluorescence, indicative of beta-sheet formation, was seen when 50 ug N-acetylated monomer and 5 ug N-acetylated fibril were combined, compared to monomer or fibril alone.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Acetyl-N-Term-Protein-In-vitro-assay-NBP3-14769-img0001.jpg "In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Acetyl (N-Term) Protein [NBP3-14769] - Thioflavin T assay for N-terminal acetylated alpha synuclein monomers and pre-formed fibrils (PFFs) (NBP3-14769). Increased fluorescence, indicative of beta-sheet formation, was seen when 50 ug N-acetylated monomer and 5 ug N-acetylated fibril were combined, compared to monomer or fibril alone.")
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Acetyl (N-Term) Protein [NBP3-14769] - TEM of N-terminal acetylated alpha synuclein pre-formed fibirls (PFFs) (NBP3-14769)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Acetyl-N-Term-Protein-Electron-Microscopy-NBP3-14769-img0003.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Acetyl (N-Term) Protein [NBP3-14769] - TEM of N-terminal acetylated alpha synuclein pre-formed fibirls (PFFs) (NBP3-14769)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Acetyl-N-Term-Protein-Electron-Microscopy-NBP3-14769-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (Y2/51) [NBP2-54418] - Formalin-fixed, paraffin-embedded human Spleen stained with CD61 Monoclonal Antibody (Y2/51).](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-Y2-51-Immunohistochemistry-Paraffin-NBP2-54418-img0001.jpg "Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (Y2/51) [NBP2-54418] - Formalin-fixed, paraffin-embedded human Spleen stained with CD61 Monoclonal Antibody (Y2/51).")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (SPM160) [NBP2-34351] - Formalin-paraffin leiomyosarcoma (20X) stained with muscle specific actin MAb (SPM160)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-SPM160-Immunohistochemistry-Paraffin-NBP2-34351-img0002.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (SPM160) [NBP2-34351] - Formalin-paraffin leiomyosarcoma (20X) stained with muscle specific actin MAb (SPM160)")
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BU20a) [NBP2-32922] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (BU20a).](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-BU20a-Immunohistochemistry-Paraffin-NBP2-32922-img0001.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BU20a) [NBP2-32922] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (BU20a).")