| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90660-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90402-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98336-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90119-50ul | |||
![Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-278202415295551.jpg "Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32199) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-298202413585510.jpg)
![Flow Cytometry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Flow cytometric analysis of Hela cells labeling HGFR/c-MET. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32199, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-29202416453259.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0003.jpg "Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0001.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunofluorescence staining of P4HB in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90660 antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-90660_rabbit-monoclonal-protein-disulfide-isomerase-p4hb-antibody-023-235202514492359.png)
![Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-278202415212474.jpg "Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32072) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-298202414333630.jpg)
![Flow Cytometry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Flow cytometric analysis of THP-1 cells labeling Bcl-2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32072, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-29202416372125.jpg)
![Immunohistochemistry-Paraffin: Cathepsin B Antibody (010) [NBP2-90402] - Staining of mouse CTSB in mouse kidney with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90402-img0002.jpg "Immunohistochemistry-Paraffin: Cathepsin B Antibody (010) [NBP2-90402] - Staining of mouse CTSB in mouse kidney with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Cathepsin B Antibody (010) [NBP2-90402] - Staining of mouse CTSB in mouse liver with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90402-img0001.jpg)
![Flow Cytometry: CD41/CD61 Antibody (PAC-1) [NBP2-62201] - Separation of CD41/CD61 positive platelets (red-filled) from CD41/CD61 negative nucleated cells (black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated human peripheral whole blood stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 ug/ml, GAM PE).](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-PAC-1-Flow-Cytometry-NBP2-62201-img0004.jpg "Flow Cytometry: CD41/CD61 Antibody (PAC-1) [NBP2-62201] - Separation of CD41/CD61 positive platelets (red-filled) from CD41/CD61 negative nucleated cells (black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated human peripheral whole blood stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 ug/ml, GAM PE).")
![Flow (Cell Surface): CD41/CD61 Antibody (PAC-1) [NBP2-62201] - Surface staining of PAC-1 on PHA-activated (1 hour) human peripheral blood with anti-PAC-1 (PAC-1) FITC.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-PAC-1-Flow-Cell-Surface-NBP2-62201-img0001.jpg)
![Flow (Cell Surface): CD41/CD61 Antibody (PAC-1) [NBP2-62201] - Surface staining pattern of PHA stimulated human peripheral whole blood stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 ug/ml, GAM PE).](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-PAC-1-Flow-Cell-Surface-NBP2-62201-img0002.jpg)
![Flow Cytometry: CD41/CD61 Antibody (PAC-1) [NBP2-62201] - Multicolor staining pattern of human lymphocytes stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 ug/ml, GAM PE) and Hoechst 34580.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-PAC-1-Flow-Cytometry-NBP2-62201-img0003.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98336-img0004.jpg "Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-98336] - Immunochemical staining of mouse E-Cadherin in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-98336-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP2-98336] - Mouse E-Cadherin was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-Mouse E-Cadherin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse E-Cadherin rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP2-98336-img0001.jpg)
![Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Western blot analysis of Actin Alpha 1 Cardiac Muscle on different lysates with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-278202415141712.jpg "Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Western blot analysis of Actin Alpha 1 Cardiac Muscle on different lysates with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Actin Alpha 1 Cardiac Muscle with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32032, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-29820241420918.jpg)
![Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32032) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-29820241420938.jpg)
![Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-27820241631678.jpg "Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32237) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-29820241435260.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunocytochemistry analysis of HeLa cells labeling Cyclin E2 with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-292024119231.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-MEM-189-Flow-Cytometry-NB500-493-img0001.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.")
![Western Blot: Cathepsin D Antibody (010) [NBP2-90119] - Anti-Cathepsin D rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: SKBR3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:45 kDa. Observed band size:31 kDa.](http://images.novusbio.com/fullsize/Cathepsin-D-Antibody-010-Western-Blot-NBP2-90119-img0004.jpg "Western Blot: Cathepsin D Antibody (010) [NBP2-90119] - Anti-Cathepsin D rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: SKBR3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:45 kDa. Observed band size:31 kDa.")
![Immunohistochemistry-Paraffin: Cathepsin D Antibody (010) [NBP2-90119] - Immunochemical staining of human CTSD in human cirrhosis with rabbit monoclonal antibody (1:300, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cathepsin-D-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90119-img0003.jpg)
![Immunohistochemistry-Paraffin: Cathepsin D Antibody (010) [NBP2-90119] - Immunochemical staining of human CTSD in human lung cancer with rabbit monoclonal antibody (1:300, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cathepsin-D-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90119-img0001.jpg)
![<b>Genetic Strategies Validation. </b>NBP2-90119 used at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Cathepsin D knockout 293T Whole Cell Lysate. Lysates loaded at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 31 kDa.](http://images.novusbio.com/fullsize/nbp2-90119_rabbit-monoclonal-cathepsin-d-antibody-010-45202316595466.png)
![Western Blot: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Western blot analysis of TIMP-1 on different lysates with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-288202412493478.jpg "Western Blot: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Western blot analysis of TIMP-1 on different lysates with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33022) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-298202414115428.jpg)
![Flow Cytometry: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Flow cytometric analysis of HT-29 cells labeling TIMP-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33022, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-392024884693.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0005.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].")
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the palatine tonsil using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0003.jpg)