| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87757-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03740-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85304-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86860-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85303-25ul | |||
![Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody [NBP1-87757] - Whole cell lysates from SUM159, MDA-MB-231 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. NF2/Merlin Antibody (NBP1-87757) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP1-87757] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87757-img0008.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human fallopian tube shows moderate cytoplasmic and moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0011.jpg)
![Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0007.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0001.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0009.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0010.jpg)
![Western Blot: LATS1 Antibody [NB200-197] - Detection of mouse LATS1 by western blot. Samples: Whole cell lysate (50 ug) from CT26.WT and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody NB200-197 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/LATS1-Antibody-Western-Blot-NB200-197-img0002.jpg "Western Blot: LATS1 Antibody [NB200-197] - Detection of mouse LATS1 by western blot. Samples: Whole cell lysate (50 ug) from CT26.WT and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody NB200-197 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: LATS1 Antibody [NB200-197] - Detection of Human LATS1 on HeLa whole cell lysate using NB200-197. LATS1 was also immunoprecipitated with another LATS1 antibody and NB200-198 using 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/LATS1-Antibody-Western-Blot-NB200-197-img0001.jpg)
![Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 180 s.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251638096.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 180 s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: NF2/Merlin Antibody [NBP3-03740] - Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug NF2/Merlin antibody (NBP3-03740). Western blot was performed from the immunoprecipitate using NF2/Merlin antibody (NBP3-03740) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunoprecipitation-NBP3-03740-img0003.jpg)
![Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of various lysates using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251634529.jpg)
![Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP3-03740] - Immunofluorescence analysis of C6 cells using NF2/Merlin antibody (NBP3-03740) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03740-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Immunofluorescence analysis of U2OS cells using [KD Validated] NF2/Merlin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-52202516283287.jpg)
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Immunofluorescence analysis of L929 cells using [KD Validated] NF2/Merlin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251628043.jpg)
![Immunohistochemistry-Paraffin: DBC1 Antibody [NBP1-85304] - Staining in human testis and liver tissues using anti-CCAR2 antibody. Corresponding CCAR2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DBC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85304-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DBC1 Antibody [NBP1-85304] - Staining in human testis and liver tissues using anti-CCAR2 antibody. Corresponding CCAR2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: DBC1 Antibody [NBP1-85304] - Staining of human kidney, liver, pancreas and testis using [NBP1-85304] (A) shows similar protein distribution across tissues to independent antibody NBP1-85305 (B).](http://images.novusbio.com/fullsize/DBC1-Antibody-Immunohistochemistry-NBP1-85304-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: DBC1 Antibody [NBP1-85304] - Analysis using [NBP1-85304] (A) shows similar pattern to independent antibody (NBP1-85305) (B).](http://images.novusbio.com/fullsize/DBC1-Antibody-Western-Blot-NBP1-85304-img0012.jpg)
![Immunohistochemistry-Paraffin: DBC1 Antibody [NBP1-85304] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/DBC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85304-img0003.jpg)
![Immunohistochemistry-Paraffin: DBC1 Antibody [NBP1-85304] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/DBC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85304-img0004.jpg)
![Immunohistochemistry-Paraffin: DBC1 Antibody [NBP1-85304] - Staining of human pancreas.](http://images.novusbio.com/fullsize/DBC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85304-img0009.jpg)
![Immunohistochemistry-Paraffin: DBC1 Antibody [NBP1-85304] - Staining of human kidney.](http://images.novusbio.com/fullsize/DBC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85304-img0010.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0011.jpg "Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0008.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0009.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscles and glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0010.jpg)
![DBC1 antibody [N1N2], N-term detects DBC1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. DBC1 antibody [N1N2], N-term (NBP3-48524) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp3-48524_rabbit-dbc1-pab-immunohistochemistry-1411202518243321.jpg "DBC1 antibody [N1N2], N-term detects DBC1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. DBC1 antibody [N1N2], N-term (NBP3-48524) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min")
![DBC1 antibody [N1N2], N-term detects DBC1 protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. DBC1 antibody [N1N2], N-term (NBP3-48524) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp3-48524_rabbit-dbc1-pab-immunohistochemistry-1411202518363618.jpg)
![DBC1 antibody [N1N2], N-term detects DBC1 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 100% MeOH for 5 min.Green: DBC1 protein stained by DBC1 antibody [N1N2], N-term (NBP3-48524) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-48524_rabbit-dbc1-pab-immunocytochemistry-immunofluorescence-141120251828112.jpg)
![Western Blot: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/2,000 dilution. Lane 1: PC-3M cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: MDA-MB-468 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: HUVEC cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: MDA-MB-231 cell lysate (negative) (20 ug/Lane) Lane 9: COS-1 cell lysate (20 ug/Lane) Lane 10: Neuro-2a cell lysate (20 ug/Lane) Lane 11: NIH/3T3 cell lysate (20 ug/Lane) Lane 12: C6 cell lysate (20 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32590) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-278202415242628.jpg "Western Blot: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/2,000 dilution. Lane 1: PC-3M cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: MDA-MB-468 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: HUVEC cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: MDA-MB-231 cell lysate (negative) (20 ug/Lane) Lane 9: COS-1 cell lysate (20 ug/Lane) Lane 10: Neuro-2a cell lysate (20 ug/Lane) Lane 11: NIH/3T3 cell lysate (20 ug/Lane) Lane 12: C6 cell lysate (20 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32590) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Immunocytochemistry analysis of HUVEC cells labeling NF2/Merlin with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-2920241192366.jpg)
![Flow Cytometry: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Flow cytometric analysis of NIH/3T3 cells labeling NF2/Merlin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32590, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-29202416372197.jpg)
![Western Blot: LATS1 Antibody [NBP3-05224] - Western blot analysis of extracts of various cell lines, using LATS1 antibody (NBP3-05224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LATS1 Antibody-Western Blot-NBP3-05224-img0002.jpg "Western Blot: LATS1 Antibody [NBP3-05224] - Western blot analysis of extracts of various cell lines, using LATS1 antibody (NBP3-05224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP3-05224] - Rat spinal cord using LATS1 Rabbit pAb (NBP3-05224) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP3-05224-img0001.jpg)
![Western Blot: LATS1 Antibody - Azide and BSA Free [LATS1] - Western blot analysis of various lysates using LATS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-05224_rabbit-polyclonal-lats1-antibody-52202516345232.jpg)
![Immunocytochemistry/Immunofluorescence: DBC1 Antibody [NBP1-85303] - Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.](http://images.novusbio.com/fullsize/DBC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85303-img0006.jpg "Immunocytochemistry/Immunofluorescence: DBC1 Antibody [NBP1-85303] - Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.")
![Immunohistochemistry-Paraffin: DBC1 Antibody [NBP1-85303] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine pancreas.](http://images.novusbio.com/fullsize/DBC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85303-img0005.jpg)
![Western Blot: DBC1 Antibody (8C10) [NBP3-42956] - fetal human brain tissue were subjected to SDS PAGE followed by western blot with (DBC1 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-42956_mouse-dbc1-mab-8c10-101220241723635.jpg "Western Blot: DBC1 Antibody (8C10) [NBP3-42956] - fetal human brain tissue were subjected to SDS PAGE followed by western blot with (DBC1 Antibody) at dilution of 1:1000")
![Immunohistochemistry: NF2/Merlin Antibody [NB100-93527] - Staining (5µg/ml) of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-NB100-93527-img0002.jpg "Immunohistochemistry: NF2/Merlin Antibody [NB100-93527] - Staining (5µg/ml) of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.")