| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76843-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56310SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76767-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22069-50ul | |||
![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0002.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0001.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-BAFF/BLyS/TNFSF13B antibody at 1 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-271120242010580.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M). Loading: 15 ug of lysates per lane.Antibodies: BAFF/BLyS/TNFSF13B (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285892.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF/BLyS/TNFSF13B with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285848.gif)
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Western-Blot-NBP1-97587-img0006.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).")
![Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Immunohistochemistry-Paraffin-NBP1-97587-img0005.jpg)
![Flow Cytometry: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x10^5) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Flow-Cytometry-NBP1-97587-img0004.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NB100-56310-img0003.jpg "Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: APRIL/TNFSF13 Antibody [NBP1-76767] - Jurkat cells with TNFSF13 antibody at 1 ug/ml (A), 2 ug/ml (B), and 4 ug/ml (C).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Western-Blot-NBP1-76767-img0004.jpg "Western Blot: APRIL/TNFSF13 Antibody [NBP1-76767] - Jurkat cells with TNFSF13 antibody at 1 ug/ml (A), 2 ug/ml (B), and 4 ug/ml (C).")
![Immunohistochemistry: APRIL/TNFSF13 Antibody - BSA Free [NBP1-76767] - Immunohistochemistry of APRIL/TNFSF13 in human prostate tissue with APRIL/TNFSF13 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76767_rabbit-polyclonal-april-tnfsf13-antibody-271120242016937.jpg)
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0002.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.")
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Detection of endogenous human APRIL with MAb to APRIL (human) (Aprily-5). Method: Cell lysate (A) or cell supernatant (B) were run on an SDS-PAGE gel and analyzed with 2ug/ml Aprily-5 by Western blot. Dendritic cells (1), NIH3T3 (2) and NIH3T3 expressing human APRIL (3).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0001.jpg)
![Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-5A8-Flow-Cytometry-NBP1-97639-img0001.jpg "Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.")
![Western Blot: APRIL/TNFSF13 Antibody (SR1578) [NBP3-22069] - Western blot analysis using NBP3-22069 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22069_rabbit-april-tnfsf13-mab-sr1578-5720231558489.jpg "Western Blot: APRIL/TNFSF13 Antibody (SR1578) [NBP3-22069] - Western blot analysis using NBP3-22069 in Jurkat cell lysate.")
![Immunocytochemistry/Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-H4-C7-Immunocytochemistry-Immunofluorescence-NBP1-04265-img0004.jpg "Immunocytochemistry/Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)")
![Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - Flow cytometry analysis of BLyS in PBMC cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-H4-C7-Flow-Cytometry-NBP1-04265-img0006.jpg)
![ELISA: Rat BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric) [NBP2-69925] - Samples were spiked with high concentrations of Rat BAFF/BLyS/TNFSF13B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69925_rat-baff-blys-tnfsf13b-elisa-kit-colorimetric-132202416204066.jpg "ELISA: Rat BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric) [NBP2-69925] - Samples were spiked with high concentrations of Rat BAFF/BLyS/TNFSF13B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric) [NBP2-69925] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69925_rat-baff-blys-tnfsf13b-elisa-kit-colorimetric-432024941517.jpg)