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![Western Blot: CYLD Antibody [NB100-78600] - A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGECYLD antibody dilution: 1:500.](http://images.novusbio.com/fullsize/CYLD-Antibody-Western-Blot-NB100-78600-img0014.jpg "Western Blot: CYLD Antibody [NB100-78600] - A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGECYLD antibody dilution: 1:500.")
![Western Blot: CYLD Antibody [NB100-78600] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody (NB100-78600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-78600_rabbit-polyclonal-cyld-antibody-910202420134080.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-25153-img0001.jpg "Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25153] - HeLa cells stained with NBP2-25153 (red) counterstained with monoclonal antibody to Hsp27, NBP2-25149 (green) and DNA (blue). NBP2-25153 reveals strong nuclear lamina staining, while NBP2-25149 reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A + C (red) are associated with the nuclear compartment, this region appears crimson in this image.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25153-img0003.jpg)
![Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-30888-img0008.jpg "Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SET Antibody [NBP1-30888] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SET protein stained by SET antibody, isoform 2-specific diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/SET-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30888-img0007.jpg)
![Immunohistochemistry-Paraffin: SET Antibody [NBP1-30888] - A549 xenograft, using SET antibody, isoform 2 specific at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SET-Antibody-Immunohistochemistry-Paraffin-NBP1-30888-img0003.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [7612].](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [7612].")
![<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 30 ug 293T B. 30 ug A431 C. 30 ug HeLa D. 30 ug HepG2 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:500.](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0001.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:1000](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0002.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:1000](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0003.jpg)
![Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [GT7612] (NBP2-43578) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43578_mouse-monoclonal-fumarase-antibody-7612-immunocytochemistry-immunofluorescence-261120251821337.jpg)
![Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0004.jpg "Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Cdk6 Antibody [NBP2-15846] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0001.jpg)
![Western Blot: Cdk6 Antibody [NBP2-15846] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0002.jpg)
![CDK6 antibody [N3C3] detects CDK6 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK6 protein stained by CDK6 antibody [N3C3] (NBP2-15846) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15846_rabbit-polyclonal-cdk6-antibody-immunocytochemistry-immunofluorescence-2611202518395722.jpg)
![Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Western-Blot-NBP2-44731-img0002.jpg "Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)")
![Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) [NBP2-44731] - Human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Immunohistochemistry-Paraffin-NBP2-44731-img0001.jpg)
![Flow Cytometry: PCNA Antibody (PCNA/694) [NBP2-44731] - Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Antibody (PCNA/694) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Flow-Cytometry-NBP2-44731-img0003.jpg)
![Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751] - Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 ug of SA1 antibody [1515]. Western blot analysis was performed using SA1 antibody [1515] EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SA1-Antibody-1515-Immunoprecipitation-NBP2-43751-img0004.jpg "Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751] - Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 ug of SA1 antibody [1515]. Western blot analysis was performed using SA1 antibody [1515] EasyBlot anti-Mouse IgG was used as a secondary reagent.")
![Western Blot: SA1 Antibody (1515) [NBP2-43751] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-91020242011502.jpg)
![Western Blot: SA1 Antibody (1515) [NBP2-43751] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-91020242014628.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-western-blot-2611202518222011.jpg)
![SA1 antibody [GT1515] detects SA1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SA1 protein stained by SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-immunocytochemistry-immunofluorescence-2611202518272316.jpg)
![Immunohistochemistry-Paraffin: NUP160 Antibody [NBP2-23718] - Analysis of NUP160 in formalin-fixed, paraffin-embedded human brain tissue using preimmune sera (top) and this antibody (bottom) at 1:100.](http://images.novusbio.com/fullsize/NUP160-Antibody-Immunohistochemistry-Paraffin-NBP2-23718-img0001.jpg "Immunohistochemistry-Paraffin: NUP160 Antibody [NBP2-23718] - Analysis of NUP160 in formalin-fixed, paraffin-embedded human brain tissue using preimmune sera (top) and this antibody (bottom) at 1:100.")
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-79022-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Simple-Western-NBP1-79022-img0003.jpg)
![Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-[p-Ser78]-Antibody-Western-Blot-NBP2-24458-img0001.jpg "Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-20356-img0004.jpg "Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
