| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11969SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99717-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99548-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89862-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98695-50ul | |||
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/OX40-Ligand-TNFSF4-Antibody-MM0505-8S23-Flow-Cytometry-NBP2-11969-img0001.jpg "Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Immunohistochemistry-Paraffin: CD86 Antibody (AP-MAB0803) [NBP2-12182] - 4% PFA fixed and paraffin embedded mouse lung tissue (LPS treated) was subjected to IHC staining using this CD86](http://images.novusbio.com/fullsize/CD86-Antibody-AP-MAB0803-Immunohistochemistry-Paraffin-NBP2-12182-img0001.jpg "Immunohistochemistry-Paraffin: CD86 Antibody (AP-MAB0803) [NBP2-12182] - 4% PFA fixed and paraffin embedded mouse lung tissue (LPS treated) was subjected to IHC staining using this CD86")
![Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-278202415242669.jpg "Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32579) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-298202413585583.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunocytochemistry analysis of SiHa cells labeling MCM6 with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-29202410234240.jpg)
![Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-278202415212410.jpg "Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32668) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-298202414333643.jpg)
![Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunocytochemistry analysis of Hela cells labeling Nucleolin with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-29202410334079.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Chk1-p-Ser296-Antibody-SN06-50-Immunohistochemistry-Paraffin-NBP2-67711-img0002.jpg "Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Chk1-p-Ser296-Antibody-SN06-50-Immunohistochemistry-Paraffin-NBP2-67711-img0001.jpg)
![Western Blot: S100A16 Antibody [NBP2-99717] - Anti-S100A16 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa](http://images.novusbio.com/fullsize/S100A16-Antibody-Western-Blot-NBP2-99717-img0006.jpg "Western Blot: S100A16 Antibody [NBP2-99717] - Anti-S100A16 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa")
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP2-99717] - Immunochemical staining of human S100A16 in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP2-99717-img0003.jpg)
![Immunoprecipitation: S100A16 Antibody [NBP2-99717] - S100A16 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate0.5 ul anti-S100A16 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-S100A16 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunoprecipitation-NBP2-99717-img0005.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP2-99717] - Immunochemical staining of human S100A16 in human brain with NBP2-99717 at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-99717_rabbit-polyclonal-s100a16-antibody-235202521451755.png)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0005.jpg "Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0001.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0002.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0003.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0004.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Western-Blot-NBP1-44893-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Immunoprecipitation-NBP1-44893-img0002.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Western-Blot-NBP2-89862-img0002.jpg "Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa")
![Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Immunoprecipitation-NBP2-89862-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: PRDX5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Knockout-Validated-NBP2-89862-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89862 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89862_rabbit-monoclonal-peroxiredoxin-5-antibody-002-235202514492332.png)
![IFITM1 Antibody [NBP2-98695] -Staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000).](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP2-98695-img0004.jpg "IFITM1 Antibody [NBP2-98695] -Staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000).")
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP2-98695] - Immunochemical staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP2-98695-img0003.jpg)
![IFITM1 Antibody [NBP2-98695] -Lane A: K562 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/nbp2-98695_rabbit-polyclonal-ifitm1-antibody-western-blot-274202312153.jpg)
![IFITM1 Antibody [NBP2-98695] -Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate 0.5uL anti-CD225 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD225 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDaObserved band size: 15 kDa](http://images.novusbio.com/fullsize/nbp2-98695_rabbit-polyclonal-ifitm1-antibody-immunoprecipitation-2742023121323.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)