| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52436-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47738-0.1mg | |||
| IHC-Prediluted | NBP2-48082 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05424-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95112-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03740-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03213-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59632 | |||
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0007.jpg "Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.")
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0003.jpg)
![Flow Cytometry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - 4: Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Flow-Cytometry-NBP2-52436-img0001.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA: 105-233)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0005.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against human CCNA2 (AA: 105-233) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0004.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0002.jpg)
![ELISA: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-ELISA-NBP2-52436-img0006.jpg)
![Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (NB100-79810) using 3 ug/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CtIP-Antibody-Immunoprecipitation-NB100-79809-img0003.jpg "Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (NB100-79810) using 3 ug/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: SNF5 Antibody [NB100-57525] - Detection of Human and Mouse SMARCB1/SNF5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NB100-57525-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: SNF5 Antibody [NB100-57525] - Detection of Human and Mouse SMARCB1/SNF5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: SNF5 Antibody [NB100-57525] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-NB100-57525-img0011.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SNF5 Antibody [NB100-57525] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-57525 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCB1/SNF5 was also immunoprecipitated by another rabbit anti-SMARCB1/SNF5 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NB100-57525-img0002.jpg)
![Immunohistochemistry: SNF5 Antibody [NB100-57525] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-NB100-57525-img0010.jpg)
![Immunoprecipitation: SNF5 Antibody [NB100-57525] - Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 (lot NB100-57525-2) used for IP at 6 ug per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody NB100-57525-1. For blotting immunoprecipitated SMARCB1/SNF5, NB100-57525 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunoprecipitation-NB100-57525-img0014.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Immunohistochemistry-Paraffin-NBP2-45144-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).")
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Simple Western lane view shows a specific band for c-Myc in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0002.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Electropherogram image of the corresponding Simple Western lane. c-Myc antibody was used at 50 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0005.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Lane view shows a specific band for c-Myc in 0.2 mg/mL of HeLa lysate and antibody at 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 74 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-45144_mouse-monoclonal-c-myc-antibody-9e10-3-1772024959410.jpg)
![Western Blot: Pin1 Antibody [NBP1-05424] - Western blot of whole HeLa cell homogenate stained with NBP1-05424, at dilution of 1:10000. A prominent band running with an apparent SDS-PAGE molecular weight of approx. 21kDa corresponds to Pin1.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NBP1-05424-img0002.jpg "Western Blot: Pin1 Antibody [NBP1-05424] - Western blot of whole HeLa cell homogenate stained with NBP1-05424, at dilution of 1:10000. A prominent band running with an apparent SDS-PAGE molecular weight of approx. 21kDa corresponds to Pin1.")
![Immunocytochemistry/Immunofluorescence: Pin1 Antibody [NBP1-05424] - HeLa cells stained with NBP1-05424 antibody to Pin-1 (1:1000 dilution, green) and monoclonal to fibrillarin, NB300-269 (red). Pin-1 stains the nuclear matrix and, much more faintly, the cytoplasm. The fibrillarin antibody marks nucleoli.](http://images.novusbio.com/fullsize/Pin1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05424-img0003.jpg)
![Western Blot: E2F8 Antibody (3E9-2F5) [H00079733-M01] - Western Blot detection against Immunogen (73.15 KDa) .](http://images.novusbio.com/fullsize/E2F8-Antibody-3E9-2F5-Western-Blot-H00079733-M01-img0001.jpg "Western Blot: E2F8 Antibody (3E9-2F5) [H00079733-M01] - Western Blot detection against Immunogen (73.15 KDa) .")
![Western Blot: n-Myc Antibody [NBP2-95112] - Analysis of extracts of HeLa cells, using MYCN antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Western-Blot-NBP2-95112-img0003.jpg "Western Blot: n-Myc Antibody [NBP2-95112] - Analysis of extracts of HeLa cells, using MYCN antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: n-Myc Antibody [NBP2-95112] - Analysis of mouse brain using MYCN Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95112-img0004.jpg)
![Immunohistochemistry: n-Myc Antibody [NBP2-95112] - Analysis of rat brain using MYCN Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Immunohistochemistry-NBP2-95112-img0005.jpg)
![Immunohistochemistry-Paraffin: n-Myc Antibody [NBP2-95112] - Mouse brain using MYCN antibody (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Immunohistochemistry-Paraffin-NBP2-95112-img0001.jpg)
![Immunohistochemistry-Paraffin: n-Myc Antibody [NBP2-95112] - Rat brain using MYCN antibody (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Immunohistochemistry-Paraffin-NBP2-95112-img0002.jpg)
![Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 180 s.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251638096.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 180 s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: NF2/Merlin Antibody [NBP3-03740] - Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug NF2/Merlin antibody (NBP3-03740). Western blot was performed from the immunoprecipitate using NF2/Merlin antibody (NBP3-03740) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunoprecipitation-NBP3-03740-img0003.jpg)
![Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of various lysates using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251634529.jpg)
![Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP3-03740] - Immunofluorescence analysis of C6 cells using NF2/Merlin antibody (NBP3-03740) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03740-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Immunofluorescence analysis of U2OS cells using [KD Validated] NF2/Merlin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-52202516283287.jpg)
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Immunofluorescence analysis of L929 cells using [KD Validated] NF2/Merlin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251628043.jpg)
![IHC-P-EGFR Antibody [NBP3-03213] - Rat liver using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123725..jpg "IHC-P-EGFR Antibody [NBP3-03213] - Rat liver using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![IHC-P-EGFR Antibody [NBP3-03213] -Human placenta using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123838..jpg)
![IHC-P-EGFR Antibody [NBP3-03213] - Mouse heart using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123952..jpg)
![Western Blot: EGFR Antibody [NBP3-03213] - analysis of A431, using EGFR Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-western-blot-611202384024..jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP3-03213] - Analysis of A-431 cells using EGFR Rabbit pAb at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunocytochemistry-immunofluorescence-611202384153..jpg)
![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0007.jpg "Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of Mouse testis, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0008.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (3E9Z3) [Cyclin A2] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Cyclin A2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15625_rabbit-cyclin-a2-mab-3e9z3-5220251731556.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (3E9Z3) [Cyclin A2] - Immunohistochemistry analysis of paraffin-embedded Human cervical squamous cell carcinoma using Cyclin A2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15625_rabbit-cyclin-a2-mab-3e9z3-52202517333839.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (3E9Z3) [Cyclin A2] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Cyclin A2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15625_rabbit-cyclin-a2-mab-3e9z3-5220251733575.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0005.jpg "Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).")
![Immunohistochemistry-Paraffin: p53 Antibody (TP53/1799R) [NBP2-59631] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained withp53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R)](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Immunohistochemistry-Paraffin-NBP2-59631-img0002.jpg)
![Flow Cytometry: p53 Antibody (TP53/1799R) [NBP2-59631] - Flow Cytometric Analysis of HeLa cells using p53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Flow-Cytometry-NBP2-59631-img0004.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0003.jpg)
![Protein Array: p53 Antibody (TP53/1799R) [NBP2-59631] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Protein-Array-NBP2-59631-img0001.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)