| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73213 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37985-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92804-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92331-0.02ml | |||
![Immunoprecipitation:Rad21 AntibodyNBP3-29598] -Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad21 antibody (NBP3-29598 lot 11) used for IP at 6 µg per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (NBP3-29598 lot 10). For blotting immunoprecipitated Rad21, NBP3-29598 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp3-29598_rabbit-rad21-pab-301220241548568.jpg "Immunoprecipitation:Rad21 AntibodyNBP3-29598] -Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad21 antibody (NBP3-29598 lot 11) used for IP at 6 µg per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (NBP3-29598 lot 10). For blotting immunoprecipitated Rad21, NBP3-29598 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunoprecipitation:Cyclin B2 AntibodyNBP3-30035] -Detection of human Cyclin B2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin B2 antibody NBP3-30035 (lot NBP3-30035-1) used for IP at 6 µg per reaction. Cyclin B2 was also immunoprecipitated by rabbit anti-Cyclin B2 antibody. For blotting immunoprecipitated Cyclin B2, BL17041 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30035_rabbit-cyclin-b2-pab-3012202412392130.jpg "Immunoprecipitation:Cyclin B2 AntibodyNBP3-30035] -Detection of human Cyclin B2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin B2 antibody NBP3-30035 (lot NBP3-30035-1) used for IP at 6 µg per reaction. Cyclin B2 was also immunoprecipitated by rabbit anti-Cyclin B2 antibody. For blotting immunoprecipitated Cyclin B2, BL17041 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: EBP1 Antibody (1D3) [NBP2-00981] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EBP1.](http://images.novusbio.com/fullsize/EBP1-Antibody-1D3-Western-Blot-NBP2-00981-img0001.jpg "Western Blot: EBP1 Antibody (1D3) [NBP2-00981] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EBP1.")
![Western Blot: CDT1 Antibody [NB100-1098] - NB100-1098 staining of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody (1 ug/ml) incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/CDT1-Antibody-Western-Blot-NB100-1098-img0004.jpg "Western Blot: CDT1 Antibody [NB100-1098] - NB100-1098 staining of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody (1 ug/ml) incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: E2F6 Antibody [NBP1-46117] - NBP1-46117 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/E2F6-Antibody-Western-Blot-NBP1-46117-img0002.jpg "Western Blot: E2F6 Antibody [NBP1-46117] - NBP1-46117 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ZMYND11 Antibody [NB100-1157] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ZMYND11-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1157-img0001.jpg "Immunocytochemistry/Immunofluorescence: ZMYND11 Antibody [NB100-1157] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Western Blot: Cdk7 Antibody [NBP2-42500] - Analysis of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NBP2-42500-img0001.jpg "Western Blot: Cdk7 Antibody [NBP2-42500] - Analysis of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PPP6C Antibody (6O2N8) [NBP3-33542] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33542_rabbit-ppp6c-mab-6o2n8-10122024140426.jpg "Western Blot: PPP6C Antibody (6O2N8) [NBP3-33542] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: PLK4 Antibody [NBP3-37985] - Western blot analysis of various lysates using PLK4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-37985_rabbit-plk4-pab-101220241134335.jpg "Western Blot: PLK4 Antibody [NBP3-37985] - Western blot analysis of various lysates using PLK4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![Western Blot: MCM4 Antibody (5N0X3) [NBP3-16791] - Western blot analysis of extracts of various cell lines, using MCM4 Rabbit mAb (NBP3-16791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MCM4-Antibody-5N0X3-Western-Blot-NBP3-16791-img0001.jpg "Western Blot: MCM4 Antibody (5N0X3) [NBP3-16791] - Western blot analysis of extracts of various cell lines, using MCM4 Rabbit mAb (NBP3-16791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CIAO1 Antibody [NBP2-92804] - Analysis of extracts of various cell lines, using CIAO1 .Exposure time: 90s.](http://images.novusbio.com/fullsize/CIAO1-Antibody-Western-Blot-NBP2-92804-img0003.jpg "Western Blot: CIAO1 Antibody [NBP2-92804] - Analysis of extracts of various cell lines, using CIAO1 .Exposure time: 90s.")
![Western Blot: CDCA5 Antibody [NBP2-92331] - Analysis of extracts of various cell lines, using CDCA5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/CDCA5-Antibody-Western-Blot-NBP2-92331-img0001.jpg "Western Blot: CDCA5 Antibody [NBP2-92331] - Analysis of extracts of various cell lines, using CDCA5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")