| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05378-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38386-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59611-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54460-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59614-100ug | |||
![Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Western-Blot-NBP3-05378-img0002.jpg "Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of C6 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-7220251245389.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432038.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432011.jpg)
![Immunohistochemistry: NDE1 Antibody [NBP3-38386] - Immunohistochemistry analysis of paraffin-embedded Rat brain using NDE1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38386_rabbit-nde1-pab-912202415121398.jpg "Immunohistochemistry: NDE1 Antibody [NBP3-38386] - Immunohistochemistry analysis of paraffin-embedded Rat brain using NDE1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: NDE1 Antibody [NBP3-38386] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NDE1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38386_rabbit-nde1-pab-912202415152870.jpg)
![Western Blot: NDE1 Antibody [NBP3-38386] - Western blot analysis of various lysates using NDE1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38386_rabbit-nde1-pab-912202414112871.jpg)
![Immunohistochemistry: NDE1 Antibody [NBP3-38386] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using NDE1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38386_rabbit-nde1-pab-912202414163071.jpg)
![Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-278202415242669.jpg "Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32579) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-298202413585583.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunocytochemistry analysis of SiHa cells labeling MCM6 with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-29202410234240.jpg)
![Western Blot: Septin-2 Antibody [NBP1-31496] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-31496-img0004.jpg "Western Blot: Septin-2 Antibody [NBP1-31496] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE, antibody diluted at 1:2000.")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-31496] - Sample: Paraffin-embedded mouse ovary. Septin-2 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31496-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-31496] - Paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31496-img0003.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-2 antibody [N1N3] (NBP1-31496) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31496_rabbit-polyclonal-septin-2-antibody-western-blot-141120251839106.jpg)
![Immunohistochemistry-Paraffin: Orc2 Antibody [NBP1-46175] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB](http://images.novusbio.com/fullsize/Orc2-Antibody-Immunohistochemistry-Paraffin-NBP1-46175-img0014.jpg "Immunohistochemistry-Paraffin: Orc2 Antibody [NBP1-46175] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Orc2 Antibody [NBP1-46175] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46175 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46176, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Orc2-Antibody-Immunoprecipitation-NBP1-46175-img0015.jpg)
![Western Blot: Cyclin E1 Antibody [NBP2-16055] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-Western-Blot-NBP2-16055-img0001.jpg "Western Blot: Cyclin E1 Antibody [NBP2-16055] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody [NBP2-16055] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cyclin E1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16055-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Cyclin E1 Antibody [NBP2-16055] - Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-Western-Blot-NBP2-16055-img0003.jpg)
![Western Blot: Cyclin E1 Antibody [NBP2-16055] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-Western-Blot-NBP2-16055-img0005.jpg)
![Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Western-Blot-NBP3-15178-img0004.jpg "Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: CDC25C Antibody (3E6) [NBP3-15178] - Immunofluorescence staining of Hela cells with at CDC25C antibody 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunocytochemistry-Immunofluorescence-NBP3-15178-img0001.jpg)
![Immunohistochemistry: CDC25C Antibody (3E6) [NBP3-15178] - IHC image of CDC25C antibody diluted at 1:165 and staining in paraffin-embedded human glioma cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunohistochemistry-NBP3-15178-img0003.jpg)
![Immunoprecipitation: CDC25C Antibody (3E6) [NBP3-15178] - Immunoprecipitating CDC25C in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of CDC25C antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: CDC25C antibody (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunoprecipitation-NBP3-15178-img0002.jpg)
![Western Blot: MCM7 Antibody (MCM7/1468) [NBP2-59610] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1468-Western-Blot-NBP2-59610-img0006.jpg "Western Blot: MCM7 Antibody (MCM7/1468) [NBP2-59610] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1468).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1468) [NBP2-59610] - Formalin-fixed, paraffin-embedded human Tonsil stained withMCM7 Mouse Monoclonal Antibody (MCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1468-Immunohistochemistry-Paraffin-NBP2-59610-img0001.jpg)
![Protein Array: MCM7 Antibody (MCM7/1468) [NBP2-59610] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Antibody (MCM7/1468) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1468-Protein-Array-NBP2-59610-img0005.jpg)
![Western Blot: MCM7 Antibody (SPM379) [NBP2-53219] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (SPM379).](http://images.novusbio.com/fullsize/MCM7-Antibody-SPM379-Western-Blot-NBP2-53219-img0002.jpg "Western Blot: MCM7 Antibody (SPM379) [NBP2-53219] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (SPM379).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (SPM379) [NBP2-53219] - Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Monoclonal Antibody (SPM379).](http://images.novusbio.com/fullsize/MCM7-Antibody-SPM379-Immunohistochemistry-Paraffin-NBP2-53219-img0001.jpg)
![Western Blot: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Western-Blot-NBP2-59613-img0004.jpg "Western Blot: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1469).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Formalin-fixed, paraffin-embedded human Tonsil stained withMCM7 Mouse Monoclonal Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Immunohistochemistry-Paraffin-NBP2-59613-img0001.jpg)
![Protein Array: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Mouse Monoclonal Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Protein-Array-NBP2-59613-img0005.jpg)
![Western Blot: MCM3 Antibody (S04-6B9) [NBP3-19485] - Western blot detection of MCM3 in Jurkat, C6, Hela cell lysates using NBP3-19485 (1:1000 diluted). Predicted band size: 91kDa. Observed band size: 100kDa.](http://images.novusbio.com/fullsize/MCM3-Antibody-S04-6B9-Western-Blot-NBP3-19485-img0003.jpg "Western Blot: MCM3 Antibody (S04-6B9) [NBP3-19485] - Western blot detection of MCM3 in Jurkat, C6, Hela cell lysates using NBP3-19485 (1:1000 diluted). Predicted band size: 91kDa. Observed band size: 100kDa.")
![Immunohistochemistry-Paraffin: MCM3 Antibody (S04-6B9) [NBP3-19485] - Immunohistochemistry of MCM3 in paraffin-embedded Human tonsil using NBP3-19485 at dilution 1/1](http://images.novusbio.com/fullsize/MCM3-Antibody-S04-6B9-Immunohistochemistry-Paraffin-NBP3-19485-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (S04-6B9) [NBP3-19485] - Immunofluorescence of MCM3 (green) in Hela cells using NBP3-19485 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19485_rabbit-mcm3-mab-s04-6b9-113202515145413.jpg)
![Immunohistochemistry: Septin-2 Antibody (8H4) [NBP3-43032] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (Septin-2 Antibody) at dilution of 1:800](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241715429.jpg "Immunohistochemistry: Septin-2 Antibody (8H4) [NBP3-43032] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (Septin-2 Antibody) at dilution of 1:800")
![Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody (8H4) [NBP3-43032] - Immunofluorescence analysis of U2OS cells using Septin-2 antibody (FNab07722). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241723695.jpg)
![Western Blot: Septin-2 Antibody (8H4) [NBP3-43032] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (Septin-2 Antibody) at 1:8000.](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241734110.jpg)