| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-00217-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03257-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34503-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34725-0.1mg | |||
![Western Blot: mTOR Antibody (F11) [NBP2-00217] - Reduced cell lysates prepared from Jurkat cell line with 5 ug/mL of Anti-Human/Mouse mTOR Purified. Bands were visualized using Anti-Mouse IgG HRP.](http://images.novusbio.com/fullsize/mTOR-Antibody-F11-Western-Blot-NBP2-00217-img0001.jpg "Western Blot: mTOR Antibody (F11) [NBP2-00217] - Reduced cell lysates prepared from Jurkat cell line with 5 ug/mL of Anti-Human/Mouse mTOR Purified. Bands were visualized using Anti-Mouse IgG HRP.")
![Western Blot: Apc1 Antibody [NB100-86985] - Detection of Human and Mouse APC1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-APC1 antibody NB100-86985 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. APC1 was also immunoprecipitated by another rabbit anti-APC1 antibody which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Apc1-Antibody-Western-Blot-NB100-86985-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Apc1 Antibody [NB100-86985] - Detection of Human and Mouse APC1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-APC1 antibody NB100-86985 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. APC1 was also immunoprecipitated by another rabbit anti-APC1 antibody which recognizes an upstream epitope.")
![Western Blot: Hexokinase 2 Antibody [NBP2-44234] - Western Blot of Rabbit Hexokinase 2 2 antibody. Lane 1: Molecular Weight Marker. Lane 2: Hexokinase 2. Primary Antibody: Anti-Hexokinase 2 at 1:1000 overnight in 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 mins at RT. Expect MW: ~54kDa.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP2-44234-img0002.jpg "Western Blot: Hexokinase 2 Antibody [NBP2-44234] - Western Blot of Rabbit Hexokinase 2 2 antibody. Lane 1: Molecular Weight Marker. Lane 2: Hexokinase 2. Primary Antibody: Anti-Hexokinase 2 at 1:1000 overnight in 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 mins at RT. Expect MW: ~54kDa.")
![ELISA: Hexokinase 2 Antibody [NBP2-44234] - ELISA Results of Rabbit Hexokinase 2 2 antibody tested against purified Hexokinase 2. Each well was coated in duplicate with 1.0 ug of Hexokinase 2 (Yeast). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-ELISA-NBP2-44234-img0001.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48667_rabbit-hexokinase-2-mab-hl2000-western-blot-1411202518324118.jpg "Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48667_rabbit-hexokinase-2-mab-hl2000-western-blot-1411202518443413.jpg)
![Zebrafish tissue extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48667_rabbit-hexokinase-2-mab-hl2000-western-blot-141120251840430.jpg)
![Whole Japanese medaka extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48667_rabbit-hexokinase-2-mab-hl2000-western-blot-141120251843314.jpg)
![Western Blot: Folliculin Antibody [NBP3-03257] - Analysis of extracts of various cell lines, using FLCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Folliculin-Antibody-Western-Blot-NBP3-03257-img0004.jpg "Western Blot: Folliculin Antibody [NBP3-03257] - Analysis of extracts of various cell lines, using FLCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: Folliculin Antibody - Azide and BSA Free [NBP3-03257] - Immunofluorescence analysis of C6 cells using Folliculin Rabbit pAb (A14521) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03257_rabbit-polyclonal-folliculin-antibody-52202519294526.jpg)
![Western Blot: Apc1 Antibody (7G9C7) [NBP2-61887] - Analysis using ANAPC1 mAb against human ANAPC1 (AA: 12-155) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/Apc1-Antibody-7G9C7-Western-Blot-NBP2-61887-img0003.jpg "Western Blot: Apc1 Antibody (7G9C7) [NBP2-61887] - Analysis using ANAPC1 mAb against human ANAPC1 (AA: 12-155) recombinant protein. (Expected MW is 41.9 kDa)")
![Flow Cytometry: Apc1 Antibody (7G9C7) [NBP2-61887] - Analysis of MCF-7 cells using ANAPC1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Apc1-Antibody-7G9C7-Flow-Cytometry-NBP2-61887-img0002.jpg)
![Western Blot: Apc1 Antibody (7G9C7) [NBP2-61887] - Analysis using ANAPC1 mAb against HEK293 (1) and ANAPC1 (AA: 12-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Apc1-Antibody-7G9C7-Western-Blot-NBP2-61887-img0004.jpg)
![ELISA: Apc1 Antibody (7G9C7) [NBP2-61887] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Apc1-Antibody-7G9C7-ELISA-NBP2-61887-img0001.jpg)
![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TS106 + TMS715) [NBP2-34447] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TS106 + TMS715).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-TS106-+-TMS715-Immunohistochemistry-Paraffin-NBP2-34447-img0001.jpg "Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TS106 + TMS715) [NBP2-34447] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TS106 + TMS715).")
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TMS715) [NBP2-34446] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TMS715).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-TMS715-Immunohistochemistry-Paraffin-NBP2-34446-img0002.jpg "Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TMS715) [NBP2-34446] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TMS715).")
![Western Blot: AIG1 Antibody - BSA Free [NBP3-48192] - Mouse testis tissue were subjected to SDS PAGE followed by western blot with (AIG1 antibody) at dilution of 1:400](http://images.novusbio.com/fullsize/nbp3-48192_rabbit-aig1-pab-bsa-free-28420251138099.jpg "Western Blot: AIG1 Antibody - BSA Free [NBP3-48192] - Mouse testis tissue were subjected to SDS PAGE followed by western blot with (AIG1 antibody) at dilution of 1:400")
![Immunohistochemistry: AIG1 Antibody - BSA Free [NBP3-48192] - Immunohistochemistry of paraffin-embedded human ovary tumor using (AIG1 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-48192_rabbit-aig1-pab-bsa-free-284202511342848.jpg)
![Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029] - Western blot detection of Fumarase in CHO-K1, C6, Jurkat cell lysates using Fumarase Rabbit mAb (1:500 diluted). Predicted band size: 55kDa. Observed band size: 49kDa.](http://images.novusbio.com/fullsize/Fumarase-Antibody-S08-3F2-Western-Blot-NBP3-15029-img0001.jpg "Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029] - Western blot detection of Fumarase in CHO-K1, C6, Jurkat cell lysates using Fumarase Rabbit mAb (1:500 diluted). Predicted band size: 55kDa. Observed band size: 49kDa.")
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody (S08-3F2) [NBP3-15029] - Immunofluorescence of Fumarase (green) in hela using Fumarase Rabbit mAb at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/Fumarase-Antibody-S08-3F2-Immunocytochemistry-Immunofluorescence-NBP3-15029-img0002.jpg)
![Western Blot: PGL2 Antibody - BSA Free [NBP3-47830] - A549 cells were subjected to SDS PAGE followed by western blot with (PGL2 Antibody) at dilution of 1:300](http://images.novusbio.com/fullsize/nbp3-47830_rabbit-pgl2-pab-bsa-free-284202511333212.jpg "Western Blot: PGL2 Antibody - BSA Free [NBP3-47830] - A549 cells were subjected to SDS PAGE followed by western blot with (PGL2 Antibody) at dilution of 1:300")
![Immunohistochemistry: PGL2 Antibody - BSA Free [NBP3-47830] - Immunohistochemistry of paraffin-embedded human testis tissue slide using (PGL2 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47830_rabbit-pgl2-pab-bsa-free-284202511342828.jpg)