| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56369SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21529-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92190-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55357-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83436-25ul | |||
![Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Western-Blot-NBP3-05295-img0004.jpg "Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295] - Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody NBP3-05295, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunocytochemistry-Immunofluorescence-NBP3-05295-img0001.jpg)
![ELISA: HDAC6 Antibody (236) [NBP3-05295] - ELISA analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295. Plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-ELISA-NBP3-05295-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295] - Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and NBP3-05295 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunoprecipitation-NBP3-05295-img0003.jpg)
![Flow Cytometry: Mouse Monoclonal HDAC6 Antibody (236) [NBP3-05295] - Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/antibody/nbp3-05295_mouse-monoclonal-hdac6-antibody-236-flow-cytometry-1112202412584..jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-26220241422521.jpg "Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-2722024850460.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate. All lanes: RBBP4/RbAp48 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 48, 47, 44 kDa Observed band size: 53, 40 kDa](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-28220241135166.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Staining of Hela Cells with RBBP4/RbAp48 Antibody (3E9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-282202412475121.jpg)
![Western Blot: HDAC7 Antibody [NB100-61587] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.4 ug/ml.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NB100-61587-img0004.jpg "Western Blot: HDAC7 Antibody [NB100-61587] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.4 ug/ml.")
![Immunohistochemistry-Paraffin: HDAC7 Antibody [NB100-61587] - Human prostate carcinoma (left) and mouse colon carcinoma (right). Antibody used at a dilution of 1:5,000.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunohistochemistry-Paraffin-NB100-61587-img0003.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NB100-569-img0014.jpg "Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NB100-569] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunohistochemistry-NB100-569-img0013.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: MAD1L1/MAD1 Antibody [NB100-569] - Whole cell lysate (50 mcg/lane in A; 50 mcg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). NB100-569 used at 0.1 mcg/ml for Western Blot (A) and at 1 mcg/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies NB100-570 and NB100-571 using 1 mcg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using NB100-570 at 0.2 mcg/ml.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunoprecipitation-NB100-569-img0009.jpg)
![Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0001.jpg "Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.")
![Western Blot: ANP32B Antibody [NB100-56369] - analysis of APRIL in HL-60 whole cell lysate using APRIL antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0002.jpg)
![Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-572023156479.jpg "Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.")
![Immunohistochemistry: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunohistochemical analysis of paraffin-embedded human breast, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-5720231558484.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-6720231051264.jpg)
![Western Blot: CtBP1 Antibody (2G7) [H00001487-M01] - CTBP1 monoclonal antibody (M01), clone 2G7 Analysis of CTBP1 expression in HL-60.](http://images.novusbio.com/fullsize/CtBP1-Antibody-2G7-Western-Blot-H00001487-M01-img0010.jpg "Western Blot: CtBP1 Antibody (2G7) [H00001487-M01] - CTBP1 monoclonal antibody (M01), clone 2G7 Analysis of CTBP1 expression in HL-60.")
![Immunocytochemistry/Immunofluorescence: CtBP1 Antibody (2G7) [H00001487-M01] - Analysis of monoclonal antibody to CTBP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CtBP1-Antibody-2G7-Immunocytochemistry-Immunofluorescence-H00001487-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: CtBP1 Antibody (2G7) [H00001487-M01] - Analysis of monoclonal antibody to CTBP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CtBP1-Antibody-2G7-Immunohistochemistry-Paraffin-H00001487-M01-img0008.jpg)
![Sandwich ELISA: CtBP1 Antibody (2G7) [H00001487-M01] - Detection limit for recombinant GST tagged CTBP1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CtBP1-Antibody-2G7-Sandwich-ELISA-H00001487-M01-img0009.jpg)
![Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Western-Blot-H00008850-M04-img0012.jpg "Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: PCAF Antibody (5E11) [H00008850-M04] - Analysis of monoclonal antibody to PCAF on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Immunocytochemistry-Immunofluorescence-H00008850-M04-img0008.jpg)
![Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in LNCaP (Cat # L004V1).](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Western-Blot-H00008850-M04-img0010.jpg)
![Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Western-Blot-H00008850-M04-img0011.jpg)
![Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in human uterus myoma.](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Western-Blot-H00008850-M04-img0013.jpg)
![Sandwich ELISA: PCAF Antibody (5E11) [H00008850-M04] - Detection limit for recombinant GST tagged PCAF is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Sandwich-ELISA-H00008850-M04-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NOC2L Antibody [NBP1-92190] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.](http://images.novusbio.com/fullsize/NOC2L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92190-img0007.jpg "Immunocytochemistry/Immunofluorescence: NOC2L Antibody [NBP1-92190] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.")
![Immunohistochemistry-Paraffin: Pit1 Antibody [NBP2-55357] - Staining of human liver, pancreas, pituitary gland and tonsil using Anti-POU1F1 antibody NBP2-55357 (A) shows similar protein distribution across tissues to independent antibody NBP1-92273 (B).](http://images.novusbio.com/fullsize/Pit1-Antibody-Immunohistochemistry-Paraffin-NBP2-55357-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Pit1 Antibody [NBP2-55357] - Staining of human liver, pancreas, pituitary gland and tonsil using Anti-POU1F1 antibody NBP2-55357 (A) shows similar protein distribution across tissues to independent antibody NBP1-92273 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Pit1 Antibody [NBP2-55357] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Pit1-Antibody-Immunohistochemistry-Paraffin-NBP2-55357-img0005.jpg)
![Immunohistochemistry-Paraffin: Pit1 Antibody [NBP2-55357] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Pit1-Antibody-Immunohistochemistry-Paraffin-NBP2-55357-img0003.jpg)
![Immunohistochemistry-Paraffin: Pit1 Antibody [NBP2-55357] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Pit1-Antibody-Immunohistochemistry-Paraffin-NBP2-55357-img0004.jpg)
![Immunohistochemistry-Paraffin: Pit1 Antibody [NBP2-55357] - Staining of human pituitary gland shows moderate to strong nuclear positivity in neuroendocrine cells in the anterior lobe.](http://images.novusbio.com/fullsize/Pit1-Antibody-Immunohistochemistry-Paraffin-NBP2-55357-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP1-83436] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles, cytosol & the Golgi apparatus.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83436-img0004.jpg "Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP1-83436] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles, cytosol & the Golgi apparatus.")
![Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP1-83436] - Staining of human skin shows moderate cytoplasmic positivity and strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-Paraffin-NBP1-83436-img0007.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP1-83436] - Staining of human placenta shows moderate cytoplasmic positivity and strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-Paraffin-NBP1-83436-img0005.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP1-83436] - Staining of human skeletal muscle shows weak cytoplasmic and nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-Paraffin-NBP1-83436-img0006.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP1-83436] - Staining of human cerebellum shows weak cytoplasmic positivity and moderate nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-Paraffin-NBP1-83436-img0008.jpg)