| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02946-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04486-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15607-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NB120-10842-0.025mg | |||
![Western Blot: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of extracts of various cell lines, using HMGN1/HMG14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Western-Blot-NBP3-02946-img0004.jpg "Western Blot: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of extracts of various cell lines, using HMGN1/HMG14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit")
![Immunocytochemistry/Immunofluorescence: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of HeLa cells using HMGN1/HMG14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02946-img0001.jpg)
![Western Blot: HRAS Antibody [NBP3-04486] - Analysis of extracts of mouse brain, using HRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04486-img0002.jpg "Western Blot: HRAS Antibody [NBP3-04486] - Analysis of extracts of mouse brain, using HRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP3-04486] - Analysis of HeLa cells using HRAS antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04486-img0001.jpg)
![Western Blot: TRRAP Antibody [NBP3-15607] - Western blot analysis of extracts of various cell lines, using TRRAP antibody (NBP3-15607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Western-Blot-NBP3-15607-img0002.jpg "Western Blot: TRRAP Antibody [NBP3-15607] - Western blot analysis of extracts of various cell lines, using TRRAP antibody (NBP3-15607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: TRRAP Antibody [NBP3-15607] - Immunohistochemistry of paraffin-embedded human esophageal cancer using TRRAP Rabbit pAb (NBP3-15607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Immunohistochemistry-Paraffin-NBP3-15607-img0001.jpg)
![Western Blot: USP22 Antibody (9C7I8) [NBP3-16794] - Western blot analysis of extracts of Mouse thymus, using USP22 Rabbit mAb (NBP3-16794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/USP22-Antibody-9C7I8-Western-Blot-NBP3-16794-img0002.jpg "Western Blot: USP22 Antibody (9C7I8) [NBP3-16794] - Western blot analysis of extracts of Mouse thymus, using USP22 Rabbit mAb (NBP3-16794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: USP22 Antibody (9C7I8) [NBP3-16794] - analysis of extracts of HepG2 cells, using USP22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16794_rabbit-usp22-mab-9c7i8-western-blot-3112023125123..jpg)
![Western Blot: USP22 Antibody (9C7I8) [NBP3-16794] - Western blot analysis of lysates from Rat lung, using USP22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-16794_rabbit-usp22-mab-9c7i8-522025121543.jpg)
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0002.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.")
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Detection of endogenous human APRIL with MAb to APRIL (human) (Aprily-5). Method: Cell lysate (A) or cell supernatant (B) were run on an SDS-PAGE gel and analyzed with 2ug/ml Aprily-5 by Western blot. Dendritic cells (1), NIH3T3 (2) and NIH3T3 expressing human APRIL (3).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0001.jpg)
![Western Blot: Oncostatin M/OSM Antibody (JE40-04) [NBP3-32679] - Western blot analysis of Oncostatin M/OSM on different lysates with Rabbit anti-Oncostatin M/OSM antibody (NBP3-32679) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate Lane 3: Hela cell lysate Lane 4: Mouse kidney tissue lysate (20 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 28 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32679) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32679_rabbit-oncostatin-m-osm-mab-je40-04-278202415242629.jpg "Western Blot: Oncostatin M/OSM Antibody (JE40-04) [NBP3-32679] - Western blot analysis of Oncostatin M/OSM on different lysates with Rabbit anti-Oncostatin M/OSM antibody (NBP3-32679) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate Lane 3: Hela cell lysate Lane 4: Mouse kidney tissue lysate (20 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 28 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32679) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Oncostatin M/OSM Antibody (JE40-04) [NBP3-32679] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Oncostatin M/OSM antibody (NBP3-32679) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32679) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32679_rabbit-oncostatin-m-osm-mab-je40-04-298202414262671.jpg)
![Western Blot: HGS Antibody [NBP2-16820] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/HGS-Antibody-Western-Blot-NBP2-16820-img0001.jpg "Western Blot: HGS Antibody [NBP2-16820] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HGS Antibody [NBP2-16820] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using HGS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/HGS-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16820-img0002.jpg)
![Western Blot: Casein Kinase 2 beta Antibody [NBP1-06515] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CKII beta antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-Western-Blot-NBP1-06515-img0001.jpg "Western Blot: Casein Kinase 2 beta Antibody [NBP1-06515] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CKII beta antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243] - Human breast carcinoma.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP2-17243-img0002.jpg "Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243] - Human breast carcinoma.")
![<b>Biological Strategies Validation. </b>Western Blot: MDA5 Antibody [NBP2-17243] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDA5 antibody [N2C1], Internal (NBP2-17243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17243_rabbit-polyclonal-mda5-antibody-910202420134057.jpg)
![Western Blot: IPP Antibody [NBP2-16984] - Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG C: SK-N-SH 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/IPP-Antibody-Western-Blot-NBP2-16984-img0001.jpg "Western Blot: IPP Antibody [NBP2-16984] - Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG C: SK-N-SH 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: IPP Antibody [NBP2-16984] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/IPP-Antibody-Immunohistochemistry-Paraffin-NBP2-16984-img0002.jpg)
![Western Blot: ADAM33 Antibody [NBP1-30898] - Sample (30 ug of whole cell lysate)A: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ADAM33-Antibody-Western-Blot-NBP1-30898-img0002.jpg "Western Blot: ADAM33 Antibody [NBP1-30898] - Sample (30 ug of whole cell lysate)A: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: ADAM33 Antibody [NBP1-30898] - Paraffin-embedded placenta. ADAM33 antibody [C2C3], C-term dilution: 1:100.](http://images.novusbio.com/fullsize/ADAM33-Antibody-Immunohistochemistry-Paraffin-NBP1-30898-img0003.jpg)