| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38164-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35293-1mg | |||
| Carrier-Free | NBP2-35293-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98826-50ul | |||
![Western Blot: FZR1/CDH1 Antibody [NBP3-38164] - Western blot analysis of lysates from 293T cells, using FZR1/CDH1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38164_rabbit-fzr1-cdh1-pab-1012202411421066.jpg "Western Blot: FZR1/CDH1 Antibody [NBP3-38164] - Western blot analysis of lysates from 293T cells, using FZR1/CDH1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP3-38164] - Immunofluorescence analysis of HeLa cells using FZR1/CDH1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38164_rabbit-fzr1-cdh1-pab-1012202411353070.jpg)
![Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CDC25C-Antibody-6R0I6-Western-Blot-NBP3-15437-img0002.jpg "Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (6R0I6) [NBP3-15437] - Immunofluorescence analysis of HeLa cells using CDC25C Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15437_rabbit-cdc25c-mab-6r0i6-72202512435382.jpg)
![SDS-Page: Rat EGF Protein [NBP2-35293]](http://images.novusbio.com/fullsize/Rat-EGF-Protein-SDS-Page-NBP2-35293-img0001.jpg "SDS-Page: Rat EGF Protein [NBP2-35293]")
![Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-278202415295577.jpg "Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HRAS Antibody (JE42-15) [NBP3-32413] - Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-298202414161896.jpg)
![Western Blot: BRD7 Antibody [NBP2-87080] - WB Suggested Anti-BRD7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateBRD7 is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP2-87080-img0002.jpg "Western Blot: BRD7 Antibody [NBP2-87080] - WB Suggested Anti-BRD7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateBRD7 is supported by BioGPS gene expression data to be expressed in Jurkat")
![Immunohistochemistry-Paraffin: BRD7 Antibody [NBP2-87080] - Rabbit Anti-BRD7 antibody. Paraffin Embedded Tissue: Human Lung. Cellular Data: alveolar cell. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/BRD7-Antibody-Immunohistochemistry-Paraffin-NBP2-87080-img0001.jpg)
![Western Blot: BRD7 Antibody [NBP2-87080] - Host: Rabbit. Target Name: BRD7. Sample Type: Jurkat. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide. Primary Antibody Concentration: 2.5ug/mL. Peptide Concentration: 2.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%BRD7](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP2-87080-img0003.jpg)
![Western Blot: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Western blot analysis of p18INK4c/CDKN2C on different lysates with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 ug/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 ug/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32690) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32690_rabbit-p18ink4c-cdkn2c-mab-je36-09-27820241631632.jpg "Western Blot: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Western blot analysis of p18INK4c/CDKN2C on different lysates with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 ug/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 ug/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32690) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32690) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32690_rabbit-p18ink4c-cdkn2c-mab-je36-09-298202414115420.jpg)
![Western Blot: KMT1A/SUV39H1 Antibody [NBP2-82268] - WB Suggested Anti-SUV39H1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysateSUV39H1 is supported by BioGPS gene expression data to be expressed in HepG2](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP2-82268-img0003.jpg "Western Blot: KMT1A/SUV39H1 Antibody [NBP2-82268] - WB Suggested Anti-SUV39H1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysateSUV39H1 is supported by BioGPS gene expression data to be expressed in HepG2")
![Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP2-82268] - Rabbit Anti-SUV39H1 Antibody. Paraffin Embedded Tissue: Human alveolar cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Immunohistochemistry-Paraffin-NBP2-82268-img0002.jpg)
![Immunohistochemistry: KMT1A/SUV39H1 Antibody [NBP2-82268] - Human Intestine](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Immunohistochemistry-NBP2-82268-img0001.jpg)
![Western Blot: TLK1 Antibody [NBP2-98826] - Lane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:87 kDaObserved band size:87 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/TLK1-Antibody-Western-Blot-NBP2-98826-img0003.jpg "Western Blot: TLK1 Antibody [NBP2-98826] - Lane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:87 kDaObserved band size:87 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: TLK1 Antibody [NBP2-98826] - Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate4 uL anti-TLK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TLK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 87 kDaObserved band size: 87 kDa](http://images.novusbio.com/fullsize/TLK1-Antibody-Immunoprecipitation-NBP2-98826-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TLK1 Antibody [NBP2-98826] - Immunofluorescence staining of TLK1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98826 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98826_rabbit-polyclonal-tlk1-antibody-235202511354115.png)
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)
![Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-298202414262624.jpg "Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-392024848501.jpg)
![Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-28820241419157.jpg "Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-UBA3 antibody (NBP3-33074) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33074) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-298202414262662.jpg)