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![Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0001.jpg)
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13683_rabbit-egfr-mab-hl1022-l858r-mutant-910202420115098.jpg "Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Various whole cell extracts (30 | 15 | 15 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13683_rabbit-egfr-mab-hl1022-l858r-mutant-91020242063323.jpg)
![Western Blot:[NBP3-29192]-Detection of human and mouse CDC37 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CDC37 antibody used for WB at 0. 4 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. CDC37 was also immunoprecipitated by rabbit anti-CDC37 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29192_rabbit-cdc37-pab-231202512101677.jpg "Western Blot:[NBP3-29192]-Detection of human and mouse CDC37 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CDC37 antibody used for WB at 0. 4 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. CDC37 was also immunoprecipitated by rabbit anti-CDC37 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.")
![Immunoprecipitation:Cyclin E2 AntibodyNBP3-29686] -Detection of human CCNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNE2 antibody NBP3-29686 (lot NBP3-29686-1) used for IP at 6 µg per reaction. CCNE2 was also immunoprecipitated by rabbit anti-CCNE2 antibody. For blotting immunoprecipitated CCNE2, NBP3-29686 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29686_rabbit-cyclin-e2-pab-301220241952587.jpg "Immunoprecipitation:Cyclin E2 AntibodyNBP3-29686] -Detection of human CCNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNE2 antibody NBP3-29686 (lot NBP3-29686-1) used for IP at 6 µg per reaction. CCNE2 was also immunoprecipitated by rabbit anti-CCNE2 antibody. For blotting immunoprecipitated CCNE2, NBP3-29686 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: SNF5 Antibody (SMARCB1/4587R) [NBP3-13776] - Formalin-fixed, paraffin-embedded human sarcoma stained with SNF5 antibody (SMARCB1/4587R)](http://images.novusbio.com/fullsize/SNF5-Antibody-SMARCB1-4587R-Immunohistochemistry-Paraffin-NBP3-13776-img0001.jpg "Immunohistochemistry-Paraffin: SNF5 Antibody (SMARCB1/4587R) [NBP3-13776] - Formalin-fixed, paraffin-embedded human sarcoma stained with SNF5 antibody (SMARCB1/4587R)")
![Immunohistochemistry-Paraffin: SNF5 Antibody (SMARCB1/4587R) [NBP3-13776] - Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with SNF5 antibody (SMARCB1/4587R).](http://images.novusbio.com/fullsize/SNF5-Antibody-SMARCB1-4587R-Immunohistochemistry-Paraffin-NBP3-13776-img0002.jpg)
![Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29063_rabbit-npm1-pab-23120251204028.jpg "Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.")
![Immunohistochemistry:[NBP3-29236]-Detection of human Cul2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul2.](http://images.novusbio.com/fullsize/nbp3-29236_rabbit-cullin-2-pab-231202511585549.jpg "Immunohistochemistry:[NBP3-29236]-Detection of human Cul2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul2.")
![Western Blot: Septin-1 Antibody (1E1) [NBP2-45762] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Septin 1.](http://images.novusbio.com/fullsize/Septin-1-Antibody-1E1-Western-Blot-NBP2-45762-img0001.jpg "Western Blot: Septin-1 Antibody (1E1) [NBP2-45762] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Septin 1.")
![Western Blot: Septin-1 Antibody (1E1) [NBP2-45762] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Septin-1-Antibody-1E1-Western-Blot-NBP2-45762-img0002.jpg)
![Western Blot: Cyclin D2 Antibody (5A6) [NBP2-46103] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cyclin D2.](http://images.novusbio.com/fullsize/Cyclin-D2-Antibody-5A6-Western-Blot-NBP2-46103-img0001.jpg "Western Blot: Cyclin D2 Antibody (5A6) [NBP2-46103] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cyclin D2.")
![Western Blot: TSH R Antibody [NBP1-53167] - Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.5 ug/ml Peptide Concentration: 2.0 ug/ml lysate Quantity: 25ug/ Lane Gel Concentration: 12% There is BioGPS gene expression data showing that TSHR is expressed in Jurkat.](http://images.novusbio.com/fullsize/TSH-R-Antibody-Western-Blot-NBP1-53167-img0005.jpg "Western Blot: TSH R Antibody [NBP1-53167] - Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.5 ug/ml Peptide Concentration: 2.0 ug/ml lysate Quantity: 25ug/ Lane Gel Concentration: 12% There is BioGPS gene expression data showing that TSHR is expressed in Jurkat.")
![Western Blot: TSH R Antibody [NBP1-53167] - Jurkat cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/TSH-R-Antibody-Western-Blot-NBP1-53167-img0002.jpg)
![NRAS Antibody [NB100-94887] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NRAS-Antibody-NB100-94887-img0001.jpg "NRAS Antibody [NB100-94887] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NB100-53805-img0001.jpg "Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")