| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11969SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99717-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98695-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99548-50ul | |||
![Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/OX40-Ligand-TNFSF4-Antibody-MM0505-8S23-Flow-Cytometry-NBP2-11969-img0001.jpg "Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Western Blot: FABP7/B-FABP Antibody [NBP3-00016] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP3-00016-img0005.jpg "Western Blot: FABP7/B-FABP Antibody [NBP3-00016] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa")
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP3-00016] - Immunochemical staining of human FABP7/B-FABP in human malignant melanoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP3-00016-img0004.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP3-00016] - Immunochemical staining of human FABP7/B-FABP in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP3-00016-img0003.jpg)
![Immunoprecipitation: FABP7/B-FABP Antibody [NBP3-00016] - Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-FABP7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-FABP7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :15 kDa](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunoprecipitation-NBP3-00016-img0006.jpg)
![Western Blot: S100A16 Antibody [NBP2-99717] - Anti-S100A16 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa](http://images.novusbio.com/fullsize/S100A16-Antibody-Western-Blot-NBP2-99717-img0006.jpg "Western Blot: S100A16 Antibody [NBP2-99717] - Anti-S100A16 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa")
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP2-99717] - Immunochemical staining of human S100A16 in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP2-99717-img0003.jpg)
![Immunoprecipitation: S100A16 Antibody [NBP2-99717] - S100A16 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate0.5 ul anti-S100A16 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-S100A16 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunoprecipitation-NBP2-99717-img0005.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP2-99717] - Immunochemical staining of human S100A16 in human brain with NBP2-99717 at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-99717_rabbit-polyclonal-s100a16-antibody-235202521451755.png)
![Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Western-Blot-NBP1-44893-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Immunoprecipitation-NBP1-44893-img0002.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![IFITM1 Antibody [NBP2-98695] -Staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000).](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP2-98695-img0004.jpg "IFITM1 Antibody [NBP2-98695] -Staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000).")
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP2-98695] - Immunochemical staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP2-98695-img0003.jpg)
![IFITM1 Antibody [NBP2-98695] -Lane A: K562 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/nbp2-98695_rabbit-polyclonal-ifitm1-antibody-western-blot-274202312153.jpg)
![IFITM1 Antibody [NBP2-98695] -Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate 0.5uL anti-CD225 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD225 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDaObserved band size: 15 kDa](http://images.novusbio.com/fullsize/nbp2-98695_rabbit-polyclonal-ifitm1-antibody-immunoprecipitation-2742023121323.jpg)
![Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-278202416331295.jpg "Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NFIB Antibody (JE35-21) [NBP3-32647] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NFIB antibody (NBP3-32647) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32647) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-298202413585588.jpg)
![Immunocytochemistry/ Immunofluorescence: NFIB Antibody (JE35-21) [NBP3-32647] - Immunocytochemistry analysis of HeLa cells labeling NFIB with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-29202410234232.jpg)
![Western Blot: CLK1 Antibody [NBP2-87194] - MCF-7 and HeLa whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. CLK1 Antibody (NBP2-87194) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CLK1-Antibody-Western-Blot-NBP2-87194-img0003.jpg "Western Blot: CLK1 Antibody [NBP2-87194] - MCF-7 and HeLa whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. CLK1 Antibody (NBP2-87194) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![Western Blot: CLK1 Antibody [NBP2-87194] - WB Suggested Anti-CLK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysateCLK1 is supported by BioGPS gene expression data to be expressed in HEK293T](http://images.novusbio.com/fullsize/CLK1-Antibody-Western-Blot-NBP2-87194-img0001.jpg)
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-278202415212410.jpg "Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32668) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-298202414333643.jpg)
![Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunocytochemistry analysis of Hela cells labeling Nucleolin with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-29202410334079.jpg)
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0005.jpg "Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0001.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0002.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0003.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0004.jpg)