| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89626-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41263-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15747-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38604-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37490-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97553-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1922SS | |||
![Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Western-Blot-NBP2-22526-img0001.jpg "Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0006.jpg)
![Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunohistochemistry-NBP2-22526-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0003.jpg)
![Immunoprecipitation: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunoprecipitation-NBP2-22526-img0002.jpg)
![Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Western-Blot-NBP2-02779-img0006.jpg "Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody (1A5) [NBP2-02779] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Immunocytochemistry-Immunofluorescence-NBP2-02779-img0005.jpg)
![Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Coronin-1a antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Flow-Cytometry-NBP2-02779-img0003.jpg)
![Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Western-Blot-NBP2-02779-img0004.jpg)
![Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - Analysis of Hela cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Flow-Cytometry-NBP2-02779-img0001.jpg)
![Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - Analysis of Jurkat cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Flow-Cytometry-NBP2-02779-img0002.jpg)
![Western Blot: ARF6 Antibody [NBP2-41263] - Analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/ARF6-Antibody-Western-Blot-NBP2-41263-img0001.jpg "Western Blot: ARF6 Antibody [NBP2-41263] - Analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: ARF6 Antibody - BSA Free [NBP2-41263] - Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41263_rabbit-polyclonal-arf6-antibody-2711202420324836.jpg)
![Immunohistochemistry: ARF6 Antibody - BSA Free [NBP2-41263] - Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41263_rabbit-polyclonal-arf6-antibody-2711202420212343.jpg)
![Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Human Lymph node (A) and Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB100-1298-img0004.jpg "Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Human Lymph node (A) and Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NB100-1298-img0002.jpg)
![Flow Cytometry: Coronin-1a Antibody [NB100-1298] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Flow-Cytometry-NB100-1298-img0005.jpg)
![Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Daudi (A), MOLT4 (B) and (1ug/ml) of Jurkat (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB100-1298-img0003.jpg)
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NB100-1298-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RhoG Antibody [NBP3-15747] - Immunofluorescence analysis of L929 cells using RhoG Rabbit pAb (NBP3-15747) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15747-img0004.jpg "Immunocytochemistry/Immunofluorescence: RhoG Antibody [NBP3-15747] - Immunofluorescence analysis of L929 cells using RhoG Rabbit pAb (NBP3-15747) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human appendix using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP3-15747-img0003.jpg)
![Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human colon carcinoma using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP3-15747-img0001.jpg)
![Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human liver cancer using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP3-15747-img0002.jpg)
![Western Blot: RhoG Antibody - Azide and BSA Free [RhoG] - Western blot analysis of various lysates using RhoG Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15747_rabbit-rhog-pab-5220251733594.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414201354.jpg "Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414201323.jpg)
![Western Blot: Coronin-1a Antibody [NBP3-38604] - Western blot analysis of various lysates using Coronin-1a Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202415152849.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414123825.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202415152850.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-91220241412386.jpg)
![Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Western-Blot-NBP2-37490-img0003.jpg "Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.")
![Immunohistochemistry: NCK1 Antibody (5B7) [NBP2-37490] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Immunohistochemistry-NBP2-37490-img0005.jpg)
![Flow Cytometry: NCK1 Antibody (5B7) [NBP2-37490] - Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Flow-Cytometry-NBP2-37490-img0004.jpg)
![Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Western-Blot-NBP2-37490-img0002.jpg)
![ELISA: NCK1 Antibody (5B7) [NBP2-37490] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-ELISA-NBP2-37490-img0001.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-278202415295566.jpg "Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP2-97553-img0003.jpg "Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa")
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP2-97553-img0004.jpg)
![Immunoprecipitation: Use1/UBE2Z Antibody [NBP2-97553] - Use1/UBE2Z was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-Use1/UBE2Z rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Use1/UBE2Z rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunoprecipitation-NBP2-97553-img0001.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody - BSA Free [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human duodenum with NBP2-97553 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-97553_rabbit-polyclonal-use1-ube2z-antibody-23520251220550.png)
![Immunocytochemistry/Immunofluorescence: Use1/UBE2Z Antibody [NBP2-97553] - Immunofluorescence staining of Use1/UBE2Z in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97553 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97553_rabbit-polyclonal-use1-ube2z-antibody-235202512205520.png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: Tubby Antibody [NB100-1922] - Detection of tub-1 (GFP complex) using NB 100-1922. Tub-1 (nr2004) was used as a negative control.](http://images.novusbio.com/fullsize/Tubby-Antibody-Western-Blot-NB100-1922-img0004.jpg "Western Blot: Tubby Antibody [NB100-1922] - Detection of tub-1 (GFP complex) using NB 100-1922. Tub-1 (nr2004) was used as a negative control.")