| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56581-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-81132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71010 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38172-20ul | |||
![Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Western-Blot-NBP2-70688-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0006.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)
![Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - CNAP1 monoclonal antibody (M01), clone 4C12 Analysis of CNAP1 expression in HeLa.](http://images.novusbio.com/fullsize/CNAP1-Antibody-4C12-Western-Blot-H00009918-M01-img0013.jpg "Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - CNAP1 monoclonal antibody (M01), clone 4C12 Analysis of CNAP1 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of monoclonal antibody to CNAP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CNAP1-Antibody-4C12-Immunocytochemistry-Immunofluorescence-H00009918-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of monoclonal antibody to CNAP1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CNAP1-Antibody-4C12-Immunohistochemistry-Paraffin-H00009918-M01-img0011.jpg)
![Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of CNAP1 expression in transfected 293T cell line by CNAP1 monoclonal antibody (M01), clone 4C12.Lane 1: CNAP1 transfected lysate(157 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CNAP1-Antibody-4C12-Western-Blot-H00009918-M01-img0014.jpg)
![Sandwich ELISA: CNAP1 Antibody (4C12) [H00009918-M01] - Detection limit for recombinant GST tagged CNAP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CNAP1-Antibody-4C12-Sandwich-ELISA-H00009918-M01-img0012.jpg)
![Western Blot: RABGAP1 Antibody [NBP2-56581] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Western-Blot-NBP2-56581-img0002.jpg "Western Blot: RABGAP1 Antibody [NBP2-56581] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: RABGAP1 Antibody [NBP2-56581] - Staining of human cell line MCF7 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56581-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-56581] - Staining in human thyroid gland and liver tissues using anti-RABGAP1 antibody. Corresponding RABGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-56581-img0005.jpg)
![Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-56581] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-56581-img0003.jpg)
![Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-56581] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-56581-img0004.jpg)
![Western Blot: ER beta/NR3A2 Antibody [NBP2-81132] - Total protein from human Adrenal gland, testis and tonsil was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ER Beta in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-Western-Blot-NBP2-81132-img0001.jpg "Western Blot: ER beta/NR3A2 Antibody [NBP2-81132] - Total protein from human Adrenal gland, testis and tonsil was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ER Beta in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NBP2-81132] - ER beta / NR3A2 was detected in paraffin-embedded sections of human breast carcinoma tissue using 1:200 Rabbit Anti-Human ER beta / NR3A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # NBP2-81132) overnight at 4 degrees C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using sodium citrate buffer (pH 6.0). Tissue was staining with DAB and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-Immunohistochemistry-Paraffin-NBP2-81132-img0002.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP2-16380-img0002.jpg "Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP2-16380] - Human intestine. ERR alpha stained by ERR alpha antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP2-16380-img0006.jpg)
![Chromatin Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Chromatin-Immunoprecipitation-NBP2-16380-img0003.jpg)
![Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 ug of ERR alpha antibody 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary eagent.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunoprecipitation-NBP2-16380-img0005.jpg)
![Western Blot: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF-1.](http://images.novusbio.com/fullsize/IGF-I-Antibody-4B12-Western-Blot-NBP2-46037-img0005.jpg "Western Blot: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF-1.")
![Immunohistochemistry: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IGF-I-Antibody-4B12-Immunohistochemistry-NBP2-46037-img0004.jpg)
![Immunohistochemistry: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IGF-I-Antibody-4B12-Immunohistochemistry-NBP2-46037-img0001.jpg)
![Immunohistochemistry: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IGF-I-Antibody-4B12-Immunohistochemistry-NBP2-46037-img0002.jpg)
![Immunohistochemistry: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/IGF-I-Antibody-4B12-Immunohistochemistry-NBP2-46037-img0003.jpg)
![Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Western-Blot-NBP3-15438-img0003.jpg "Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry: Aromatase Antibody (5C5N7) [NBP3-15438] - Immunofluorescence analysis of human placenta using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-NBP3-15438-img0002.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody (5C5N7) [NBP3-15438] - Human colon using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-Paraffin-NBP3-15438-img0001.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-52202517273588.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-5220251723570.jpg)
![Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Western-Blot-NBP2-15086-img0004.jpg "Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE; antibody diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: RABGAP1 Antibody [NBP2-15086] - Analysis of methanol-fixed HeLa, using RABGAP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15086-img0001.jpg)
![Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-15086] - Paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-15086-img0002.jpg)
![Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Western-Blot-NBP2-15086-img0003.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (C-542) [NBP1-19318] - Analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-C-542-Western-Blot-NBP1-19318-img0002.jpg "Western Blot: ER alpha/NR3A1 Antibody (C-542) [NBP1-19318] - Analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).")
![Western Blot: GnRH Antibody [NBP3-38172] - Western blot analysis of lysates from Mouse lung, using GnRH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38172_rabbit-gnrh-pab-912202414213740.jpg "Western Blot: GnRH Antibody [NBP3-38172] - Western blot analysis of lysates from Mouse lung, using GnRH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: GnRH Antibody [NBP3-38172] - Western blot analysis of lysates from Rat kidney, using GnRH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38172_rabbit-gnrh-pab-912202415175233.jpg)
![Immunocytochemistry/ Immunofluorescence: GnRH Antibody [NBP3-38172] - Immunofluorescence analysis of SH-SY5Y cells using GnRH Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38172_rabbit-gnrh-pab-912202415111874.jpg)
![Immunocytochemistry/ Immunofluorescence: GnRH Antibody [NBP3-38172] - Immunofluorescence analysis of NIH/3T3 cells using GnRH Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38172_rabbit-gnrh-pab-3112025173131.jpg%3Ft=1690980791)