| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73930 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77171-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92546-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44217 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44231 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44221 | |||
| BSA Free | NBP3-07986-25ul | |||
![Western Blot: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROR beta.](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Western-Blot-NBP2-45610-img0013.jpg "Western Blot: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROR beta.")
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0012.jpg)
![Immunohistochemistry: ROR beta Antibody (OTI1G1) [NBP2-45610] - ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0001.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Adenocarcinoma of Human colon. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0002.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0003.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0004.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0005.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0006.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0007.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Human pancreas. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0008.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0009.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0010.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human prostate tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0011.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Western-Blot-NBP2-71351-img0001.jpg "Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0009.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0002.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0003.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0004.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0005.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0006.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0007.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0008.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0010.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0012.jpg "Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Immunocytochemistry-Immunofluorescence-H00004782-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Immunohistochemistry-Paraffin-H00004782-M01-img0009.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # H00004782-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0010.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - NFIC monoclonal antibody (M01), clone 1C12-2A2 Analysis of NFIC expression in A-431.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0011.jpg)
![Western Blot: IFITM1 Antibody [NBP1-77171] - 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Western-Blot-NBP1-77171-img0001.jpg "Western Blot: IFITM1 Antibody [NBP1-77171] - 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: IFITM1 Antibody [NBP1-77171] - Jurkat cells with IFITM1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77171-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171] - Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77171_rabbit-polyclonal-ifitm1-antibody-2711202419451297.jpg)
![Western Blot: DSPP Antibody - Azide and BSA Free [NBP2-92546] - Western blot analysis of various lysates using DSPP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92546_rabbit-polyclonal-dspp-antibody-4220251430494.jpg%3Ft=1711070358 "Western Blot: DSPP Antibody - Azide and BSA Free [NBP2-92546] - Western blot analysis of various lysates using DSPP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of RH-30 cells using the Anti-HDAC5 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/HDAC5-Antibody-CL8121-Immunocytochemistry-Immunofluorescence-NBP2-88913-img0001.jpg "Immunocytochemistry/Immunofluorescence: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of RH-30 cells using the Anti-HDAC5 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HDAC5-Antibody-CL8121-Immunohistochemistry-Paraffin-NBP2-88913-img0006.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/HDAC5-Antibody-CL8121-Immunohistochemistry-Paraffin-NBP2-88913-img0002.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/HDAC5-Antibody-CL8121-Immunohistochemistry-Paraffin-NBP2-88913-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/HDAC5-Antibody-CL8121-Immunohistochemistry-Paraffin-NBP2-88913-img0004.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human stomach cancer shows moderate to strong cytoplasmic and nuclear positivity.](http://images.novusbio.com/fullsize/HDAC5-Antibody-CL8121-Immunohistochemistry-Paraffin-NBP2-88913-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Analysis in human cerebral cortex and liver tissues using NBP2-88913 antibody. Corresponding HDAC5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HDAC5-Antibody-CL8121-Immunohistochemistry-Paraffin-NBP2-88913-img0007.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-278202415295566.jpg "Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-28820241423313.jpg "Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29820241435223.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29202410484898.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-39202488464.jpg)
![Western Blot: KLF10 Antibody (1H3) [H00007071-M15] - Analysis of KLF10 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/KLF10-Antibody-1H3-Western-Blot-H00007071-M15-img0013.jpg "Western Blot: KLF10 Antibody (1H3) [H00007071-M15] - Analysis of KLF10 expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: KLF10 Antibody (1H3) [H00007071-M15] - Analysis of KLF10 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/KLF10-Antibody-1H3-Western-Blot-H00007071-M15-img0008.jpg)
![Western Blot: KLF10 Antibody (1H3) [H00007071-M15] - Analysis of KLF10 expression in A-549 (Cat # L025V1).](http://images.novusbio.com/fullsize/KLF10-Antibody-1H3-Western-Blot-H00007071-M15-img0009.jpg)
![Western Blot: KLF10 Antibody (1H3) [H00007071-M15] - Analysis of KLF10 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/KLF10-Antibody-1H3-Western-Blot-H00007071-M15-img0010.jpg)
![Western Blot: KLF10 Antibody (1H3) [H00007071-M15] - Analysis of KLF10 expression in Y-79 (Cat # L042V1).](http://images.novusbio.com/fullsize/KLF10-Antibody-1H3-Western-Blot-H00007071-M15-img0011.jpg)
![Western Blot: KLF10 Antibody (1H3) [H00007071-M15] - Analysis of KLF10 expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/KLF10-Antibody-1H3-Western-Blot-H00007071-M15-img0012.jpg)
![Western Blot: KLF10 Antibody (1H3) [H00007071-M15] - Analysis of KLF10 expression in human colon.](http://images.novusbio.com/fullsize/KLF10-Antibody-1H3-Western-Blot-H00007071-M15-img0014.jpg)
![Immunohistochemistry-Paraffin: FGF18 Antibody (CL7628) [NBP2-88869] - Analysis in human heart muscle and liver tissues using AMAb91480 antibody. Corresponding FGF18 RNA-seq data are presented for the same tissues.i](http://images.novusbio.com/fullsize/FGF18-Antibody-CL7628-Immunohistochemistry-Paraffin-NBP2-88869-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FGF18 Antibody (CL7628) [NBP2-88869] - Analysis in human heart muscle and liver tissues using AMAb91480 antibody. Corresponding FGF18 RNA-seq data are presented for the same tissues.i")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FGF18 Antibody (CL7628) [NBP2-88869] - Analysis in human plasma.](http://images.novusbio.com/fullsize/FGF18-Antibody-CL7628-Western-Blot-NBP2-88869-img0002.jpg)
![Immunohistochemistry-Paraffin: FGF18 Antibody (CL7628) [NBP2-88869] - Staining of human heart muscle shows strong positivity of secretion around cardiomyocytes.](http://images.novusbio.com/fullsize/FGF18-Antibody-CL7628-Immunohistochemistry-Paraffin-NBP2-88869-img0007.jpg)
![Immunohistochemistry-Paraffin: FGF18 Antibody (CL7628) [NBP2-88869] - Staining in human Fallopian tube shows moderate positivity of secretion under the gladular cell layer.](http://images.novusbio.com/fullsize/FGF18-Antibody-CL7628-Immunohistochemistry-Paraffin-NBP2-88869-img0005.jpg)
![Immunohistochemistry-Paraffin: FGF18 Antibody (CL7628) [NBP2-88869] - Stainin of human testis shows moderate positivity of secretion around peritubular myiod cells.](http://images.novusbio.com/fullsize/FGF18-Antibody-CL7628-Immunohistochemistry-Paraffin-NBP2-88869-img0004.jpg)
![Immunohistochemistry-Paraffin: FGF18 Antibody (CL7628) [NBP2-88869] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FGF18-Antibody-CL7628-Immunohistochemistry-Paraffin-NBP2-88869-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cerebral cortex and liver tissues using NBP3-07986 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cerebral cortex and liver tissues using NBP3-07986 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Western-Blot-NBP3-07986-img0008.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human cerebral cortex shows strong cytoplasmic positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0004.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglia and nerves.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0005.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of rat brain shows strong cytoplasmic positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0006.jpg)