| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26167-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92902-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54673-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33854-25ul | |||
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202413221512.jpg "Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202414471576.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-2722024820395.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4H7) [NBP3-26167] - Staining of Hela cells with Lamin A + C Antibody (4H7) at 1:38, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202412493637.jpg)
![Flow Cytometry: Lamin A + C Antibody (4H7) [NBP3-26167] - Overlay histogram showing Hela cells stained with Lamin A + C Antibody (4H7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202414312758.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in mouse brain.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0008.jpg "Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in mouse brain.")
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in PC-12.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0004.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in IMR-32.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0005.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in human liver.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0006.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in mouse kidney.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0007.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in transfected 293T cell line by CENPH polyclonal antibody.Lane 1: CENPH transfected lysate(27.28 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0009.jpg)
![Western Blot: CENPH Antibody [H00064946-B02P] - Analysis of CENPH expression in NIH/3T3.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-B02P-img0007.jpg "Western Blot: CENPH Antibody [H00064946-B02P] - Analysis of CENPH expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: CENPH Antibody [H00064946-B02P] - Analysis of purified antibody to CENPH on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/CENPH-Antibody-Immunocytochemistry-Immunofluorescence-H00064946-B02P-img0004.jpg)
![Western Blot: CENPH Antibody [H00064946-B02P] - Analysis of CENPH expression in HeLa.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-B02P-img0005.jpg)
![Western Blot: CENPH Antibody [H00064946-B02P] - Analysis of CENPH expression in Raw 264.7.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-B02P-img0006.jpg)
![Western Blot: CENPH Antibody [H00064946-B02P] - Analysis of CENPH expression in transfected 293T cell line by CENPH polyclonal antibody. Lane 1: CENPH transfected lysate(27.17 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-B02P-img0008.jpg)
![Western Blot: Cyclin C Antibody [NBP2-92902] - Analysis of extracts of various cell lines, using Cyclin C at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Western-Blot-NBP2-92902-img0005.jpg "Western Blot: Cyclin C Antibody [NBP2-92902] - Analysis of extracts of various cell lines, using Cyclin C at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded rat brain using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded human colon carcinoma using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded human lung cancer using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0003.jpg)
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Western-Blot-NBP1-18878-img0004.jpg "Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.")
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP1-18878] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-18878-img0003.jpg)
![Immunoprecipitation: Cyclin C Antibody [NBP1-18878] - Detection of human Cyclin C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin C antibody NBP1-18878 used for IP at 6 ug per reaction. For blotting immunoprecipitated Cyclin C, NBP1-18878 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunoprecipitation-NBP1-18878-img0005.jpg)
![Western Blot: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Western blot detection of Lamin A + C in 3T3, Hela cell lysates using NBP3-19963 (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 74, 63kDa.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-S06-7H5-Western-Blot-NBP3-19963-img0003.jpg "Western Blot: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Western blot detection of Lamin A + C in 3T3, Hela cell lysates using NBP3-19963 (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 74, 63kDa.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Immunohistochemistry of Lamin A + C in paraffin-embedded Human breast cancer tissue using NBP3-19963 at dilution 1/5](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-S06-7H5-Immunohistochemistry-Paraffin-NBP3-19963-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Immunofluorescence of Lamin A + C (green) in Hela cells using NBP3-19963 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19963_rabbit-lamin-a-c-mab-s06-7h5-113202515145450.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human cerebral cortex, colon, kidney and pancreas using Anti-NUMA1 antibody NBP2-54673 (A) shows similar protein distribution across tissues to independent antibody NBP2-54672 (B).](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human cerebral cortex, colon, kidney and pancreas using Anti-NUMA1 antibody NBP2-54673 (A) shows similar protein distribution across tissues to independent antibody NBP2-54672 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0006.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human kidney.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0004.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human colon.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0005.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human pancreas.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HGS Antibody [NBP1-83203] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HGS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83203-img0007.jpg "Immunocytochemistry/Immunofluorescence: HGS Antibody [NBP1-83203] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83203] - Staining of human skeletal muscle shows strong cytoplasmic positivity in subsets of muscle fibers.](http://images.novusbio.com/fullsize/HGS-Antibody-Immunohistochemistry-Paraffin-NBP1-83203-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CTCF Antibody [NBP2-33854] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33854-img0005.jpg "Immunocytochemistry/Immunofluorescence: CTCF Antibody [NBP2-33854] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry: CTCF Antibody [NBP2-33854] - Staining of human colon shows strong nuclear and moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunohistochemistry-NBP2-33854-img0002.jpg)
![Western Blot: PELO Antibody [NBP3-12192] - WB of NBP3-12192 on Mouse liver. 1:500 antibody dilution in DiluObuffer. Apparent MW is 47 kDa.](http://images.novusbio.com/fullsize/PELO-Antibody-Western-Blot-NBP3-12192-img0001.jpg "Western Blot: PELO Antibody [NBP3-12192] - WB of NBP3-12192 on Mouse liver. 1:500 antibody dilution in DiluObuffer. Apparent MW is 47 kDa.")
![Immunohistochemistry-Paraffin: PELO Antibody [NBP3-12192] - 1:100 dilution IHC Blobking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000B. FFPE section.](http://images.novusbio.com/fullsize/PELO-Antibody-Immunohistochemistry-Paraffin-NBP3-12192-img0002.jpg)