| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03921-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03257-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35288-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37510-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61769-0.025ml | |||
![Western Blot: MORF4L2 Antibody [NBP3-03921] - Western blot analysis of extracts of Jurkat cells, using MORF4L2 antibody (NBP3-03921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MORF4L2 Antibody-Western Blot-NBP3-03921-img0002.jpg "Western Blot: MORF4L2 Antibody [NBP3-03921] - Western blot analysis of extracts of Jurkat cells, using MORF4L2 antibody (NBP3-03921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: MORF4L2 Antibody - BSA Free [NBP3-03921] - Immunofluorescence analysis of A549 cells using MORF4L2 antibody (A7577).](http://images.novusbio.com/fullsize/nbp3-03921_rabbit-polyclonal-morf4l2-antibody-52202519294542.jpg)
![Western Blot: Folliculin Antibody [NBP3-03257] - Analysis of extracts of various cell lines, using FLCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Folliculin-Antibody-Western-Blot-NBP3-03257-img0004.jpg "Western Blot: Folliculin Antibody [NBP3-03257] - Analysis of extracts of various cell lines, using FLCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: Folliculin Antibody - Azide and BSA Free [NBP3-03257] - Immunofluorescence analysis of C6 cells using Folliculin Rabbit pAb (A14521) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03257_rabbit-polyclonal-folliculin-antibody-52202519294526.jpg)
![Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-10122024120667.jpg "Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk7 Antibody [NBP3-35288] - Western Blot analysis of lysates from wild type (WT) and Cdk7 knockout (KO) 293T cells, using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-31120251744483.jpg%3Ft=1709093634)
![Analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-western-blot-2332026125530.png "Analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.")
![Analysis of various lysates using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-western-blot-2332026125718.png)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (0D0C8) [p16INK4a/CDKN2A] - Confocal imaging of HeLa cells using [KO Validated] p16INK4a/CDKN2A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-52202517284956.jpg)
![Western Blot: LRP-5 Antibody (2B11) [NBP2-37510] - Western blot analysis using LRP5 mAb against human LRP5 (AA: 1422-1615) recombinant protein. (Expected MW is 20.8 kDa)](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-Western-Blot-NBP2-37510-img0002.jpg "Western Blot: LRP-5 Antibody (2B11) [NBP2-37510] - Western blot analysis using LRP5 mAb against human LRP5 (AA: 1422-1615) recombinant protein. (Expected MW is 20.8 kDa)")
![Flow Cytometry: LRP-5 Antibody (2B11) [NBP2-37510] - Flow cytometric analysis of HeLa cells using LRP5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-Flow-Cytometry-NBP2-37510-img0003.jpg)
![ELISA: LRP-5 Antibody (2B11) [NBP2-37510] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-ELISA-NBP2-37510-img0001.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0003.jpg "Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)")
![Flow Cytometry: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Flow-Cytometry-NBP2-61769-img0002.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-Western-Blot-NBP2-61769-img0004.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (1A6E11) [NBP2-61769] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-1A6E11-ELISA-NBP2-61769-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58367] - Anti-Coronin 1A](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58367-img0003.jpg "Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58367] - Anti-Coronin 1A")
![Western Blot: BRD7 Antibody [NBP2-87080] - WB Suggested Anti-BRD7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateBRD7 is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP2-87080-img0002.jpg "Western Blot: BRD7 Antibody [NBP2-87080] - WB Suggested Anti-BRD7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateBRD7 is supported by BioGPS gene expression data to be expressed in Jurkat")
![Immunohistochemistry-Paraffin: BRD7 Antibody [NBP2-87080] - Rabbit Anti-BRD7 antibody. Paraffin Embedded Tissue: Human Lung. Cellular Data: alveolar cell. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/BRD7-Antibody-Immunohistochemistry-Paraffin-NBP2-87080-img0001.jpg)
![Western Blot: BRD7 Antibody [NBP2-87080] - Host: Rabbit. Target Name: BRD7. Sample Type: Jurkat. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide. Primary Antibody Concentration: 2.5ug/mL. Peptide Concentration: 2.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%BRD7](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP2-87080-img0003.jpg)
![Western Blot: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Western blot analysis of p18INK4c/CDKN2C on different lysates with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 ug/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 ug/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32690) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32690_rabbit-p18ink4c-cdkn2c-mab-je36-09-27820241631632.jpg "Western Blot: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Western blot analysis of p18INK4c/CDKN2C on different lysates with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 ug/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 ug/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32690) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32690) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32690_rabbit-p18ink4c-cdkn2c-mab-je36-09-298202414115420.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-278202416331268.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32689) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-298202414333625.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunocytochemistry analysis of SKOV-3 cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-29202410484819.jpg)
![Western Blot: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Western blot analysis of FABP3/H-FABP on different lysates with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-278202416285235.jpg "Western Blot: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Western blot analysis of FABP3/H-FABP on different lysates with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32325) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-29820241435241.jpg)
![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)