| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16925-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35185-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92447-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74027-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04567-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35222-20ul | |||
![Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/TSC2-[p-Ser1387]-Antibody-Western-Blot-NBP3-16925-img0001.jpg "Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: CDC123 Antibody [NBP3-35185] - Western blot analysis of various lysates using CDC123 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35185_rabbit-cdc123-pab-912202414163075.jpg "Western Blot: CDC123 Antibody [NBP3-35185] - Western blot analysis of various lysates using CDC123 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TSC2-Antibody-7X2C6-Western-Blot-NBP3-15763-img0001.jpg "Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: TSC2 Antibody (7X2C6) [NBP3-15763] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug TSC2 antibody (NBP3-15763). Western blot was performed from the immunoprecipitate using TSC2 antibody (NBP3-15763) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TSC2-Antibody-7X2C6-Immunoprecipitation-NBP3-15763-img0002.jpg)
![Western Blot: dynein, cytoplasmic 2, light intermediate chain 1 Antibody [NBP2-92447] - Analysis of extracts of various cell lines, using dynein, cytoplasmic 2, light intermediate chain 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/dynein--cytoplasmic-2--light-intermediate-chain-1-Antibody-Western-Blot-NBP2-92447-img0001.jpg "Western Blot: dynein, cytoplasmic 2, light intermediate chain 1 Antibody [NBP2-92447] - Analysis of extracts of various cell lines, using dynein, cytoplasmic 2, light intermediate chain 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")
![Western Blot: p57 Kip2 Antibody (6T4E6) [NBP3-16648] - Analysis of extracts of various cell lines, using p57 Kip2 Rabbit mAb (NBP3-16648) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-6T4E6-Western-Blot-NBP3-16648-img0001.jpg "Western Blot: p57 Kip2 Antibody (6T4E6) [NBP3-16648] - Analysis of extracts of various cell lines, using p57 Kip2 Rabbit mAb (NBP3-16648) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Western-Blot-NBP1-74027-img0005.jpg "Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B")
![Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Flow-Cytometry-NBP1-74027-img0006.jpg)
![Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Pin1-Antibody-3G8-Western-Blot-NBP1-22956-img0002.jpg "Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Flow Cytometry: Pin1 Antibody (3G8) [NBP1-22956] - Analysis of Pin1 in Jurkat cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Pin1-Antibody-3G8-Flow-Cytometry-NBP1-22956-img0003.jpg)
![Western Blot: IFRD1 Antibody [NBP3-04567] - Analysis of extracts of various cell lines, using IFRD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL BasicECL Basic Kit](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP3-04567-img0002.jpg "Western Blot: IFRD1 Antibody [NBP3-04567] - Analysis of extracts of various cell lines, using IFRD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL BasicECL Basic Kit")
![Western Blot: Hexokinase 2 Antibody (1A7) [NBP1-04296] - A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-1A7-Western-Blot-NBP1-04296-img0003.jpg "Western Blot: Hexokinase 2 Antibody (1A7) [NBP1-04296] - A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection.")
![Western Blot: TCP1-eta Antibody [NBP3-35222] - Western blot analysis of various lysates using TCP1-eta Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35222_rabbit-tcp1-eta-pab-912202415143664.jpg "Western Blot: TCP1-eta Antibody [NBP3-35222] - Western blot analysis of various lysates using TCP1-eta Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot: Cdk9 [p Thr186] Antibody [NBP3-05455] - Western blot analysis of extracts of NIH/3T3, using Cdk9 antibody (NBP3-05455) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Cdk9-[p-Thr186]-Antibody-Western-Blot-NBP3-05455-img0001.jpg "Western Blot: Cdk9 [p Thr186] Antibody [NBP3-05455] - Western blot analysis of extracts of NIH/3T3, using Cdk9 antibody (NBP3-05455) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")