| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76698-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92963-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92881-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38162-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61768-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81096-0.025mg | |||
![Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Analysis of IL-27 in A-20 cell lysate with IL-27 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: IL-27 NBP1-76698 (4 ug/mL), IL-27 NBP1-76412 (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-27 Antibody - BSA Free [NBP1-76698] - Validation of IL-27 in Mouse EL4 Cells. Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76698_rabbit-polyclonal-il-27-antibody-2711202420212370.jpg)
![Western Blot: CIRBP Antibody [NBP1-52067] - NBP1-52067 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CIRBP-Antibody-Western-Blot-NBP1-52067-img0003.jpg "Western Blot: CIRBP Antibody [NBP1-52067] - NBP1-52067 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CIRBP Antibody [NBP1-52067] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-Immunohistochemistry-Paraffin-NBP1-52067-img0004.jpg)
![Western Blot: Hexim 1 Antibody [NB100-55391] - Staining of human (A) and mouse (B) heart lysates (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Western-Blot-NB100-55391-img0006.jpg "Western Blot: Hexim 1 Antibody [NB100-55391] - Staining of human (A) and mouse (B) heart lysates (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody [NB100-55391] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55391-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody [NB100-55391] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55391-img0007.jpg)
![Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Western-Blot-NBP1-51946-img0006.jpg "Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of paraffin embedded Human Prostate with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP1-51946-img0005.jpg)
![Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts of various cell lines, using ARHGEF1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Western-Blot-NBP2-92963-img0003.jpg "Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts of various cell lines, using ARHGEF1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP2-92963] - Human tonsil using ARHGEF1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Immunohistochemistry-Paraffin-NBP2-92963-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Knockout-Validated-NBP2-92963-img0002.jpg)
![Western Blot: CDKN3 Antibody - Azide and BSA Free [NBP2-92881] - Western blot analysis of lysates from Mouse liver using CDKN3 Rabbit pAb at 1:2000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit.Exposuretime:60s.](http://images.novusbio.com/fullsize/nbp2-92881_rabbit-polyclonal-cdkn3-antibody-42202514251890.jpg%3Ft=1709191907 "Western Blot: CDKN3 Antibody - Azide and BSA Free [NBP2-92881] - Western blot analysis of lysates from Mouse liver using CDKN3 Rabbit pAb at 1:2000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit.Exposuretime:60s.")
![Western Blot: CDKN3 Antibody - Azide and BSA Free [NBP2-92881] - Western blot analysis of lysates from MCF7 cells, using CDKN3 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp2-92881_rabbit-polyclonal-cdkn3-antibody-42202514275269.jpg%3Ft=1709191907)
![Immunocytochemistry/ Immunofluorescence: CDKN3 Antibody - Azide and BSA Free [NBP2-92881] - Immunofluorescence analysis of MCF7 cells using CDKN3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92881_rabbit-polyclonal-cdkn3-antibody-42202514275236.jpg%3Ft=1709191907)
![Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-91220241519565.jpg "Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation: RFC4 Antibody [NBP3-38162] - Immunoprecipitation analysis of 200 ug extracts of K562 cells using RFC4 antibody. Western blot was performed from the immunoprecipitate using RFC4 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202414201340.jpg)
![Western Blot: RFC4 Antibody [NBP3-38162] - Western blot analysis of various lysates using RFC4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202415143626.jpg)
![Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202415175266.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0004.jpg "Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0003.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0005.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0001.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0002.jpg)
![Western Blot: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG.](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-OTI2C5-Azide-and-BSA-Free-Western-Blot-NBP2-71735-img0001.jpg "Western Blot: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG.")
![Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-OTI2C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71735-img0003.jpg)
![Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-OTI2C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71735-img0002.jpg)
![Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) - Azide and BSA Free [NBP2-71735] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-OTI2C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71735-img0004.jpg)
![Western Blot: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/IL-6-Antibody-1-6-Western-Blot-NBP2-89149-img0001.jpg "Western Blot: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of EL4 cells labeling IL-6 with Rabbit anti-IL-6 antibody at 1/1,000 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-6 antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp2-89149_mouse-monoclonal-il-6-antibody-1-6-immunocytochemistry-immunofluorescence-266202412753..jpg)
![Western Blot: ILF3 Antibody [NBP1-71876] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NF90/NF110 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies NBP1-71874 and NBP1-71875 as well as anti-NF110 antibody NBP1-71877, each of which recognizes a different epitope.](http://images.novusbio.com/fullsize/ILF3-Antibody-Western-Blot-NBP1-71876-img0001.jpg "Western Blot: ILF3 Antibody [NBP1-71876] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NF90/NF110 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies NBP1-71874 and NBP1-71875 as well as anti-NF110 antibody NBP1-71877, each of which recognizes a different epitope.")
![Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-71876] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- NF90/NF110 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ILF3-Antibody-Immunohistochemistry-Paraffin-NBP1-71876-img0002.jpg)