| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35190-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92658-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52580-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37351-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97936-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98122-50ul | |||
![Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP3-35190] - Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35190_rabbit-calpain-1-pab-912202415175220.jpg "Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP3-35190] - Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: Calpain 1 Antibody [NBP3-35190] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Calpain 1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35190_rabbit-calpain-1-pab-912202414154360.jpg)
![Western Blot: Calpain 1 Antibody [NBP3-35190] - Western blot analysis of various lysates using Calpain 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35190_rabbit-calpain-1-pab-9122024159527.jpg)
![Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP3-35190] - Immunofluorescence analysis of HeLa cells using Calpain 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35190_rabbit-calpain-1-pab-9122024159598.jpg)
![Western Blot: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Western blot analysis of extracts of various cell lines, using Oncostatin M/OSM Rabbit mAb (NBP3-16686) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Oncostatin-M-OSM-Antibody-8O8K9-Western-Blot-NBP3-16686-img0004.jpg "Western Blot: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Western blot analysis of extracts of various cell lines, using Oncostatin M/OSM Rabbit mAb (NBP3-16686) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Immunofluorescence analysis of Jurkat cells using Oncostatin M/OSM Rabbit mAb (NBP3-16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Oncostatin-M-OSM-Antibody-8O8K9-Immunocytochemistry-Immunofluorescence-NBP3-16686-img0001.jpg)
![Immunohistochemistry: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Immunofluorescence analysis of mouse spleen using Oncostatin M/OSM Rabbit mAb (NBP3-16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Oncostatin-M-OSM-Antibody-8O8K9-Immunohistochemistry-NBP3-16686-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Immunofluorescence analysis of paraffin-embedded rat spleen using Oncostatin M/OSM Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16686_rabbit-oncostatin-m-osm-mab-8o8k9-52202511383752.jpg)
![Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92658] - Western blot analysis of extracts of various cell lines, using COMP/Thrombospondin-5 antibody (NBP2-92658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/COMP-Thrombospondin-5-Antibody-Western-Blot-NBP2-92658-img0003.jpg "Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92658] - Western blot analysis of extracts of various cell lines, using COMP/Thrombospondin-5 antibody (NBP2-92658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92658] - Western blot analysis of extracts of various cell lines, using COMP/Thrombospondin-5 antibody (NBP2-92658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/COMP-Thrombospondin-5 Antibody-Western Blot-NBP2-92658-img0004.jpg)
![Immunohistochemistry: COMP/Thrombospondin-5 Antibody [NBP2-92658] - Immunofluorescence analysis of Mouse cartilage using COMP/Thrombospondin-5 Rabbit pAb (NBP2-92658) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/COMP-Thrombospondin-5-Antibody-Immunohistochemistry-NBP2-92658-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: COMP/Thrombospondin-5 Antibody - Azide and BSA Free [NBP2-92658] - Immunofluorescence analysis of Rat cartilage using COMP/Thrombospondin-5 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92658_rabbit-polyclonal-comp-thrombospondin-5-antibody-4220251430488.jpg%3Ft=1695694289)
![Western Blot: Ets-1 Antibody (8A8) [NBP2-22169] - Western blot analysis using ETS1 mouse mAb against Jurkat (1), HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/Ets-1-Antibody-8A8-Western-Blot-NBP2-22169-img0004.jpg "Western Blot: Ets-1 Antibody (8A8) [NBP2-22169] - Western blot analysis using ETS1 mouse mAb against Jurkat (1), HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ets-1 Antibody (8A8) [NBP2-22169] - Immunofluorescence analysis of NIH/3T3 cells using anti-ETS1 mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Ets-1-Antibody-8A8-Immunocytochemistry-Immunofluorescence-NBP2-22169-img0002.jpg)
![Immunohistochemistry-Paraffin: Ets-1 Antibody (8A8) [NBP2-22169] - Immunohistochemical analysis of paraffin-embedded human Colon, Adipocytes tissues using anti-ETS1 mouse mAb](http://images.novusbio.com/fullsize/Ets-1-Antibody-8A8-Immunohistochemistry-Paraffin-NBP2-22169-img0003.jpg)
![Flow Cytometry: Ets-1 Antibody (8A8) [NBP2-22169] - Flow cytometric analysis of Jurkat cells using anti-ETS1 mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Ets-1-Antibody-8A8-Flow-Cytometry-NBP2-22169-img0001.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mouse mAb against Hela (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0006.jpg "Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mouse mAb against Hela (1), and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis of paraffin-embedded esophageal cancer tissues using UBE2C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Immunohistochemistry-Paraffin-NBP2-52580-img0002.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mAb against human UBE2C (AA: FULL(1-179)) recombinant protein. (Expected MW is 45.6 kDa)](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0003.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mAb against HEK293 (1) and UBE2C (AA: FULL(1-179))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0004.jpg)
![Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis of paraffin-embedded liver cancer tissues using UBE2C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Immunohistochemistry-Paraffin-NBP2-52580-img0001.jpg)
![ELISA: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-ELISA-NBP2-52580-img0005.jpg)
![Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mouse mAb against PANC1 (1) and PC-3 (2) cell lysate.](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Western-Blot-NBP2-37351-img0004.jpg "Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mouse mAb against PANC1 (1) and PC-3 (2) cell lysate.")
![Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using GPNMB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Immunohistochemistry-NBP2-37351-img0006.jpg)
![Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mAb against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Western-Blot-NBP2-37351-img0002.jpg)
![Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mAb against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Western-Blot-NBP2-37351-img0003.jpg)
![Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPNMB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Immunohistochemistry-NBP2-37351-img0005.jpg)
![ELISA: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-ELISA-NBP2-37351-img0001.jpg)
![Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29820241420932.jpg "Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: EGFR Antibody (PD00-75) [NBP3-32255] - Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32255) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-2920241192377.jpg)
![Flow Cytometry: EGFR Antibody (PD00-75) [NBP3-32255] - Flow cytometric analysis of A431 cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32255, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29202416372141.jpg)
![Western Blot: EGFR Antibody (PD00-75) [NBP3-32255] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32255) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MDA-MB-468 cell lysate Lane 4: MCF7 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 134 kDa Observed band size: 150 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-3920248485012.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-278202416331227.jpg "Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32350) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29820241435263.jpg)
![Immunocytochemistry/ Immunofluorescence: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunocytochemistry analysis of U-87 MG cells labeling FRA-1/FOSL1 with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202410484854.jpg)
![Flow Cytometry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Flow cytometric analysis of U-87 MG cells labeling FRA-1/FOSL1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32350, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202416453260.jpg)
![Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-97936-img0005.jpg "Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-97936] - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97936-img0004.jpg)
![Immunoprecipitation: RFC4 Antibody [NBP2-97936] - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunoprecipitation-NBP2-97936-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-97936] - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97936 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97936_rabbit-polyclonal-rfc4-antibody-23520251664787.png)
![Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-98122-img0002.jpg "Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse intestine with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0004.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0003.jpg)
![Immunoprecipitation: CDC37 Antibody [NBP2-98122] - Mouse CDC37 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-Mouse CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse CDC37 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-98122-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of CDC37 in mouse kidney with NBP2-98122 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-98122_rabbit-polyclonal-cdc37-antibody-23520252135161.png)
![Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0004.jpg "Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Human tonsil tissue using anti-IP10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunohistochemistry-Paraffin-NBP2-67004-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0003.jpg)
![Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Analysis of paraffin-embedded human skin tissue using anti-IP10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunohistochemistry-Paraffin-NBP2-67004-img0002.jpg)
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)