| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76487-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35799-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92733-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52492-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99443-50ul | |||
![Western Blot: Pappalysin-2/PAPP-A2 Antibody [NBP1-76487] - HeLa cell lysate at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Pappalysin-2-PAPP-A2-Antibody-Western-Blot-NBP1-76487-img0001.jpg "Western Blot: Pappalysin-2/PAPP-A2 Antibody [NBP1-76487] - HeLa cell lysate at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: Pappalysin-2/PAPP-A2 Antibody - BSA Free [NBP1-76487] - Immunofluorescence of Pappalysin-2/PAPP-A2 in HeLa cells with Pappalysin-2/PAPP-A2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76487_rabbit-polyclonal-pappalysin-2-papp-a2-antibody-2711202420324861.jpg)
![Immunocytochemistry/ Immunofluorescence: Pappalysin-2/PAPP-A2 Antibody - BSA Free [NBP1-76487] - Immunocytochemistry of Pappalysin-2/PAPP-A2 in HeLa cells with Pappalysin-2/PAPP-A2 antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76487_rabbit-polyclonal-pappalysin-2-papp-a2-antibody-271120242016949.jpg)
![Western Blot: Fumarase Antibody [NB100-61663] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NB100-61663-img0001.jpg "Western Blot: Fumarase Antibody [NB100-61663] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Fumarase Antibody [NB100-61663] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NB100-61663-img0003.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NB100-61663] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NB100-61663-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Western-Blot-NBP3-16844-img0003.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunofluorescence analysis of C6 cells using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunocytochemistry-Immunofluorescence-NBP3-16844-img0002.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunohistochemistry of paraffin-embedded rat kidney using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunohistochemistry-Paraffin-NBP3-16844-img0001.jpg)
![Immunoprecipitation: HGS Antibody [NBP3-35799] - Immunoprecipitation analysis of 300 ug extracts of HepG2 cells using 3 ug HGS antibody. Western blot was performed from the immunoprecipitate using HGS antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35799_rabbit-hgs-pab-91220241418114.jpg "Immunoprecipitation: HGS Antibody [NBP3-35799] - Immunoprecipitation analysis of 300 ug extracts of HepG2 cells using 3 ug HGS antibody. Western blot was performed from the immunoprecipitate using HGS antibody at a dilution of 1:1000.")
![Immunocytochemistry/ Immunofluorescence: HGS Antibody [NBP3-35799] - Immunofluorescence analysis of U-2 OS cells using HGS Rabbit pAb at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35799_rabbit-hgs-pab-91220241519575.jpg)
![Western Blot: HGS Antibody [NBP3-35799] - Western blot analysis of various lysates using HGS Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35799_rabbit-hgs-pab-912202414123810.jpg)
![Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of extracts of various cell lines, using COMP/Thrombospondin-5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/COMP-Thrombospondin-5-Antibody-Western-Blot-NBP2-92733-img0003.jpg "Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of extracts of various cell lines, using COMP/Thrombospondin-5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of L929 cells using COMP Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/COMP-Thrombospondin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92733-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of Human Dermal Fibroblasts (HDFa). Green: 1:100 COMP/Thrombospondin-5 antibody in PBS/BSA 1%, 1h room temperature Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-92733_rabbit-polyclonal-comp-thrombospondin-5-antibody-162023124536.jpg)
![Western Blot: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Analysis using TARDBP mAb against human TARDBP (AA: 126-260) recombinant protein. (Expected MW is 41.5 kDa)](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-7F9A6-Western-Blot-NBP2-52492-img0004.jpg "Western Blot: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Analysis using TARDBP mAb against human TARDBP (AA: 126-260) recombinant protein. (Expected MW is 41.5 kDa)")
![Flow Cytometry: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Analysis of Hela cells using TARDBP mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-7F9A6-Flow-Cytometry-NBP2-52492-img0001.jpg)
![Western Blot: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Analysis using TARDBP mAb against HEK293 (1) and TARDBP (AA: 126-260)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-7F9A6-Western-Blot-NBP2-52492-img0003.jpg)
![ELISA: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-7F9A6-ELISA-NBP2-52492-img0005.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Western-Blot-NBP1-22410-img0005.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Immunocytochemistry-Immunofluorescence-NBP1-22410-img0006.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0004.jpg "Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in cynomolgus heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0001.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in human colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0002.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0003.jpg)
![Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-27820241631678.jpg "Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32237) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-29820241435260.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunocytochemistry analysis of HeLa cells labeling Cyclin E2 with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-292024119231.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western Blot: CIAO1 Antibody [NBP2-84682] - Host: Rabbit. Target Name: CIAO1. Sample Type: MCF7. Antibody Dilution: 1.0ug/mlCIAO1 is supported by BioGPS gene expression data to be expressed in MCF7](http://images.novusbio.com/fullsize/CIAO1-Antibody-Western-Blot-NBP2-84682-img0003.jpg "Western Blot: CIAO1 Antibody [NBP2-84682] - Host: Rabbit. Target Name: CIAO1. Sample Type: MCF7. Antibody Dilution: 1.0ug/mlCIAO1 is supported by BioGPS gene expression data to be expressed in MCF7")
![Immunohistochemistry-Paraffin: CIAO1 Antibody [NBP2-84682] - Rabbit Anti-CIAO1 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue. Observed Staining: Cytoplasmic in alveolar type I cells. Primary Antibody Concentration: 1:100. Other Working Concentrations: 1/600. Secondary Antibody: Donkey anti-Ra](http://images.novusbio.com/fullsize/CIAO1-Antibody-Immunohistochemistry-Paraffin-NBP2-84682-img0001.jpg)
![Western Blot: CIAO1 Antibody [NBP2-84682] - WB Suggested Anti-CIAO1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysateCIAO1 is supported by BioGPS gene expression data to be expressed in 721_B](http://images.novusbio.com/fullsize/CIAO1-Antibody-Western-Blot-NBP2-84682-img0002.jpg)
![Western Blot: CIAO1 Antibody [NBP2-84682] - Host: Rabbit. Target Name: CIAO1. Sample Type: 721_B. Antibody Dilution: 1.0ug/mlCIAO1 is supported by BioGPS gene expression data to be expressed in 721_B](http://images.novusbio.com/fullsize/CIAO1-Antibody-Western-Blot-NBP2-84682-img0004.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)