| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96431-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44974 | |||
| Azide and BSA Free | NBP2-34751-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71079 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87321-25ul | |||
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0009.jpg "Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42496-img0008.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42496] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42496-img0016.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0004.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A) and Rat (B) Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0005.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42496-img0007.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0007.jpg "Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of Hela cells using Bmi1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunocytochemistry-Immunofluorescence-NBP2-22204-img0002.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Flow-Cytometry-NBP2-22204-img0001.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0006.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0003.jpg)
![ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-ELISA-NBP2-22204-img0005.jpg)
![Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Knockout-Validated-NB110-96431-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Load: 15 ug protein. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Western-Blot-NB110-96431-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (8A7) [NB110-96431] - analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Courtesy of: Mike Welsh, Umich.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunocytochemistry-Immunofluorescence-NB110-96431-img0007.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0016.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (8A7) [NB110-96431] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-Paraffin-NB110-96431-img0008.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0017.jpg)
![Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-FC82291-Western-Blot-NB600-1030-img0001.jpg "Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse")
![Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunofluorescence-NB600-1030-img0002.jpg)
![Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-B23 Clone: FC82291 Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunoblotting-NB600-1030-img0008.jpg)
![Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Western-Blot-NBP2-32865-img0005.jpg "Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (SPM252) [NBP2-32865] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunocytochemistry-Immunofluorescence-NBP2-32865-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0006.jpg)
![Flow Cytometry: HSP27 Antibody (SPM252) [NBP2-32865] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (SPM252).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Flow-Cytometry-NBP2-32865-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (20X) stained with HSP27 MAb (SPM252)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (10X) stained with HSP27 MAb (SPM252))](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0001.jpg)
![Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Positive lysates LY409579 (100ug) and LC409579 (20ug).](http://images.novusbio.com/fullsize/KRAS-Antibody-OTI2C1-Western-Blot-NBP2-45536-img0004.jpg "Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS. Positive lysates LY409579 (100ug) and LC409579 (20ug).")
![Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536] - Staining of paraffin-embedded human normal kidney using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. NBP2-45536 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show mostly cytoplasmic staining.](http://images.novusbio.com/fullsize/KRAS-Antibody-OTI2C1-Immunohistochemistry-Paraffin-NBP2-45536-img0012.jpg)
![Western Blot: KRAS Antibody (OTI2C1) [NBP2-45536] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/KRAS-Antibody-2C1-Western-Blot-NBP2-45536-img0002.jpg)
![Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536] - Staining of human normal brain using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. NBP2-45536 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.](http://images.novusbio.com/fullsize/KRAS-Antibody-OTI2C1-Immunohistochemistry-Paraffin-NBP2-45536-img0006.jpg)
![Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536] - Staining of human normal colon using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. NBP2-45536 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.](http://images.novusbio.com/fullsize/KRAS-Antibody-OTI2C1-Immunohistochemistry-Paraffin-NBP2-45536-img0008.jpg)
![Immunohistochemistry-Paraffin: KRAS Antibody (OTI2C1) [NBP2-45536] - Staining of human normal brain using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. NBP2-45536 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.](http://images.novusbio.com/fullsize/KRAS-Antibody-OTI2C1-Immunohistochemistry-Paraffin-NBP2-45536-img0010.jpg)
![KRAS Antibody (OTI2C1)[NBP2-45536]Simple Western� analysis of endogenous protein DCPS from HELA lysates (0.2 mg/mL) using DCPS Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-45536_mouse-kras-mab-oti2c1-simple-western-1311202512362213.jpg%253fstore=america&image-type=image)
![Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.](http://images.novusbio.com/fullsize/HSP27-Antibody-Western-Blot-NB120-5579-img0003.jpg "Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0008.jpg)
![Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of extracts of various cell lines, using HMGB2 Rabbit mAb (NBP3-16774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Western-Blot-NBP3-16774-img0007.jpg "Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of extracts of various cell lines, using HMGB2 Rabbit mAb (NBP3-16774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunoprecipitation: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of 300ug extracts of Mouse testis cells using 3ug HMGB2 antibody (NBP3-16774). Western blot was performed from the immunoprecipitate using HMGB2 (NBP3-16774) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Immunoprecipitation-NBP3-16774-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774] - Confocal imaging of U-2 OS cells using HMGB2 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16774_rabbit-hmgb2-mab-8p10v5-52202511392298.jpg)
![Immunohistochemistry: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using HMGB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16774_rabbit-hmgb2-mab-8p10v5-5220251138910.jpg)
![Immunohistochemistry: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using HMGB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16774_rabbit-hmgb2-mab-8p10v5-52202511411417.jpg)
![Immunohistochemistry: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HMGB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16774_rabbit-hmgb2-mab-8p10v5-52202511365118.jpg)
![Immunohistochemistry: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunohistochemistry analysis of paraffin-embedded Human colon using HMGB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16774_rabbit-hmgb2-mab-8p10v5-522025125494.jpg)
![Immunohistochemistry: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using HMGB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16774_rabbit-hmgb2-mab-8p10v5-52202511374110.jpg)
![Chromatin Immunoprecipitation: HMGB2 Antibody (8P10V5) [NBP3-16774] - Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using HMGB2 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16774_rabbit-hmgb2-mab-8p10v5-522025126536.jpg)
![Western Blot: ATF5 Antibody (SD2099) [NBP2-67767] - Western blot analysis of ATF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Western-Blot-NBP2-67767-img0008.jpg "Western Blot: ATF5 Antibody (SD2099) [NBP2-67767] - Western blot analysis of ATF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767] - Staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunocytochemistry-Immunofluorescence-NBP2-67767-img0007.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767] - Staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunocytochemistry-Immunofluorescence-NBP2-67767-img0001.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0002.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0003.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0004.jpg)
![Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Western-Blot-NBP2-67381-img0008.jpg "Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunocytochemistry-Immunofluorescence-NBP2-67381-img0002.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0007.jpg)
![Flow Cytometry: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of Hela cells with MLH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Flow-Cytometry-NBP2-67381-img0001.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0005.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - All lanes: Western blot analysis of MLH1 with anti-MLH1 antibody [SP08-04] at 1:1,000 dilution. Lane 1: Wild-type B16F10 whole cell lysate. Lane 2: MLH1 knockout B16F10 whole cell lysate. NBP2-67381 was shown to specifically react with MLH1 in wild-type B16F10 cells. No band was observed when MLH1 knockout samples were tested. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MLH1 antibody (1/1,000) and Anti-Vinculin antibody (1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Knockout-Validated-NBP2-67381-img0010.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0012.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0007.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0008.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0010.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human prostate shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0011.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0013.jpg)