| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88832-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15747-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37490-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03736-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90134-25ul | |||
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human testis.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0009.jpg "Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human testis.")
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon, kidney, testis and urinary bladder using Anti-MKKS antibody NBP1-92122 (A) shows similar protein distribution across tissues to independent antibody NBP1-92123 (B).](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0006.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0007.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0008.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human kidney.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0010.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-92122_rabbit-polyclonal-mkks-antibody-29520251625315.jpg)
![Western Blot: RhoG Antibody [NBP1-88832] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/RhoG-Antibody-Western-Blot-NBP1-88832-img0004.jpg "Western Blot: RhoG Antibody [NBP1-88832] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: RhoG Antibody [NBP1-88832] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP1-88832-img0007.jpg)
![Western Blot: RhoG Antibody [NBP1-88832] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/RhoG-Antibody-Western-Blot-NBP1-88832-img0005.jpg)
![Immunohistochemistry-Paraffin: RhoG Antibody [NBP1-88832] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP1-88832-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RhoG Antibody [NBP1-88832] - Staining in human bone marrow and pancreas tissues using anti-RHOG antibody. Corresponding RHOG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP1-88832-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Immunocytochemistry-Immunofluorescence-NBP3-13505-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (NBP3-13505) diluted by 1:1000.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0003.jpg)
![beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13505_mouse-beta-iii-tubulin-mab-gt1338-immunohistochemistry-paraffin-2611202518393116.jpg)
![Immunocytochemistry/Immunofluorescence: RhoG Antibody [NBP3-15747] - Immunofluorescence analysis of L929 cells using RhoG Rabbit pAb (NBP3-15747) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15747-img0004.jpg "Immunocytochemistry/Immunofluorescence: RhoG Antibody [NBP3-15747] - Immunofluorescence analysis of L929 cells using RhoG Rabbit pAb (NBP3-15747) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human appendix using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP3-15747-img0003.jpg)
![Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human colon carcinoma using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP3-15747-img0001.jpg)
![Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human liver cancer using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoG-Antibody-Immunohistochemistry-Paraffin-NBP3-15747-img0002.jpg)
![Western Blot: RhoG Antibody - Azide and BSA Free [RhoG] - Western blot analysis of various lysates using RhoG Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15747_rabbit-rhog-pab-5220251733594.jpg)
![Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Western-Blot-NBP2-37490-img0003.jpg "Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.")
![Immunohistochemistry: NCK1 Antibody (5B7) [NBP2-37490] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Immunohistochemistry-NBP2-37490-img0005.jpg)
![Flow Cytometry: NCK1 Antibody (5B7) [NBP2-37490] - Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Flow-Cytometry-NBP2-37490-img0004.jpg)
![Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Western-Blot-NBP2-37490-img0002.jpg)
![ELISA: NCK1 Antibody (5B7) [NBP2-37490] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-ELISA-NBP2-37490-img0001.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-28820241423313.jpg "Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29820241435223.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29202410484898.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-39202488464.jpg)
![Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-2120249374941.jpg "Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein at nucleus by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:250. Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-2120249422615.jpg)
![Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-212024934278.jpg)
![NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518405413.jpg)
![NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518394124.jpg)
![NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-immunohistochemistry-paraffin-141120251841529.jpg)
![Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Western-Blot-NBP3-16759-img0003.jpg "Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Immunocytochemistry-Immunofluorescence-NBP3-16759-img0002.jpg)
![Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunohistochemistry of paraffin-embedded rat testis using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Immunohistochemistry-Paraffin-NBP3-16759-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16759_rabbit-nck1-mab-4r1n9-52202511374179.jpg)
![Western Blot: Exostosin 1 Antibody [NBP3-03736] - Analysis of extracts of various cell lines, using Exostosin 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Exostosin-1-Antibody-Western-Blot-NBP3-03736-img0002.jpg "Western Blot: Exostosin 1 Antibody [NBP3-03736] - Analysis of extracts of various cell lines, using Exostosin 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of NIH/3T3 cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-1620238270..jpg)
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of PC-12 cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-16202382850..jpg)
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of U2OS cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-16202383040..jpg)
![Immunocytochemistry/ Immunofluorescence: Exostosin 1 Antibody - Azide and BSA Free [NBP3-03736] - Immunofluorescence analysis of HeLa cells using Exostosin 1 Rabbit pAb (A2030) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-52202519313480.jpg)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Analysis in human skeletal muscle and liver tissues using NBP1-90134 antibody. Corresponding SPEG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0015.jpg "Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Analysis in human skeletal muscle and liver tissues using NBP1-90134 antibody. Corresponding SPEG RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0010.jpg)
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Staining of human endometrium shows strong membranous positivity in stromal cells.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0012.jpg)
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0013.jpg)
![Immunohistochemistry-Paraffin: APEG1 Antibody [NBP1-90134] - Staining of human skeletal muscle shows moderate membranous positivity in striated muscle fibers.](http://images.novusbio.com/fullsize/APEG1-Antibody-Immunohistochemistry-Paraffin-NBP1-90134-img0014.jpg)