| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22832SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54592-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26180-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB5729-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56736SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21579-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21529-50ul | |||
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)
![Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-22832-img0004.jpg "Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.")
![Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20--p-Ser24]-Antibody-Western-Blot-NBP1-78102-img0001.jpg "Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.")
![Immunohistochemistry: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20-p-Ser24]-Antibody-Immunohistochemistry-NBP1-78102-img0003.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Immunohistochemistry-Paraffin-NBP2-53181-img0004.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (rWT1/857).")
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded human Testis stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Immunohistochemistry-Paraffin-NBP2-53181-img0002.jpg)
![Protein Array: WT1 Antibody (rWT1/857) [NBP2-53181] - Analysis of Protein Array containing more than 19,000 full-length human proteins using WT1 Antibody (rWT1/857).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Protein-Array-NBP2-53181-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/nbp2-53181_mouse-monoclonal-wt1-antibody-rwt1-857-1772024938132.jpg)
![HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunoprecipitation-NBP2-16508-img0003.jpg "HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp2-16508_rabbit-polyclonal-flotillin-1-antibody-western-blot-8620239419.jpg)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16508_rabbit-polyclonal-flotillin-1-antibody-western-blot-305202593537.png)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (NBP2-16508) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16508_rabbit-polyclonal-flotillin-1-antibody-western-blot-261120251953523.jpg)
![Western Blot: DLG5 Antibody [NBP1-28739] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DLG5 antibody NBP1-28739 used for WB at 0.1 ug/mL (A) and 1 ug/mL (B) and used for IP at 10 ug/mg lysate. DLG5 was also immunoprecipitated by rabbit anti-DLG5 antibody NBP1-28694, which recognizes an upstream epitope. Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/DLG5-Antibody-Western-Blot-NBP1-28739-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DLG5 Antibody [NBP1-28739] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DLG5 antibody NBP1-28739 used for WB at 0.1 ug/mL (A) and 1 ug/mL (B) and used for IP at 10 ug/mg lysate. DLG5 was also immunoprecipitated by rabbit anti-DLG5 antibody NBP1-28694, which recognizes an upstream epitope. Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DLG5 Antibody [NBP1-28739] - IP of overexpressed CitK (citron-kinase) in HEK293 cell line. DLG5 is co-immunoprecipitated with the DLG5 antibody but not with control beads or with a control isotypic antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DLG5-Antibody-Immunoprecipitation-NBP1-28739-img0002.jpg)
![Immunoprecipitation: KLF4 Antibody (3A1) [NBP3-26180] - Immunoprecipitating KLF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26180 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26180 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-262202413221520.jpg "Immunoprecipitation: KLF4 Antibody (3A1) [NBP3-26180] - Immunoprecipitating KLF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26180 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26180 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] - Image of KLF4 Antibody (3A1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-262202414471565.jpg)
![Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] - Image of KLF4 Antibody (3A1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-27220248274912.jpg)
![Western Blot: KLF4 Antibody (3A1) [NBP3-26180] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, Brain tissue. All lanes: KLF4 Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 52, 46, 13, 7 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-28220241141363.jpg)
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (3A1) [NBP3-26180] - Staining of Hela Cells with KLF4 Antibody (3A1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-2822024131222.jpg)
![Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Immunocytochemistry-MAB5729-img0002.jpg "Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.")
![Flow Cytometry: WT1 Antibody (960522) [MAB5729] - An intracellular stain was performed on THP-1 cells with WT1 [960522] Antibody MAB5729AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Flow-Cytometry-MAB5729-img0003.jpg)
![Western Blot: RGS2 Antibody [NB100-56736] - Western bot analysis of RGS2 in human heart lysate using RGS2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RGS2-Antibody-Western-Blot-NB100-56736-img0002.jpg "Western Blot: RGS2 Antibody [NB100-56736] - Western bot analysis of RGS2 in human heart lysate using RGS2 antibody at 2 ug/ml.")
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0002.jpg "Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: EBP1 Antibody [NBP2-16257] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16257-img0003.jpg)
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0001.jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP2-16257 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-91020242021926.jpg)
![Immunohistochemistry: PA2G4/EBP1 Antibody [NBP2-16257] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PA2G4/EBP1 antibody [N3C3] (NBP2-16257) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-9102024201579.jpg)
![Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using NBP3-21579.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-5720231526114.jpg "Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using NBP3-21579.")
![Immunohistochemistry: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunohistochemical analysis of paraffin-embedded human cervix cancer, using NBP3-21579.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-57202315545129.jpg)
![Western Blot: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Western blot analysis using NBP3-21579 in Jurkat cell lysates treated with Pervanadate.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-5720231649421.jpg)
![Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-572023156479.jpg "Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.")
![Immunohistochemistry: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunohistochemical analysis of paraffin-embedded human breast, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-5720231558484.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-6720231051264.jpg)