| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-60979-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98781-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06419-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98881-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98832-50ul | |||
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58367] - Anti-Coronin 1A](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58367-img0003.jpg "Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58367] - Anti-Coronin 1A")
![Western Blot: PDGFA Antibody (MM0013-6D35) - Azide and BSA Free [NB110-60979] - Lysate of human placenta tissue with mouse anti-human PDGFA antibody.](http://images.novusbio.com/fullsize/PDGFA-Antibody-MM0013-6D35-Azide-and-BSA-Free-Western-Blot-NB110-60979-img0002.jpg "Western Blot: PDGFA Antibody (MM0013-6D35) - Azide and BSA Free [NB110-60979] - Lysate of human placenta tissue with mouse anti-human PDGFA antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: PDGFA Antibody (MM0013-6D35) [NB110-60979] - Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using NB110-60979 (Proteinase K pretreatment is required).](http://images.novusbio.com/fullsize/PDGFA-Antibody-MM0013-6D35-Immunohistochemistry-Paraffin-NB110-60979-img0001.jpg)
![Western blot analysis on different lysates with NBP3-32175 at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32175 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32175_-western-blot-639204590076866489.jpg "Western blot analysis on different lysates with NBP3-32175 at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32175 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: NCAPH Antibody [NBP2-98781] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:82 kDaObserved band size:110 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP2-98781-img0001.jpg "Western Blot: NCAPH Antibody [NBP2-98781] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:82 kDaObserved band size:110 kDa")
![Immunoprecipitation: NCAPH Antibody [NBP2-98781] - Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-NCAPH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NCAPH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 83 kDaObserved band size: 110 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunoprecipitation-NBP2-98781-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP2-98781] - Staining of NCAPH in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-98781 antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98781_rabbit-polyclonal-ncaph-antibody-23520251135415.png)
![Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 Antibody was immunoprecipitated using: Lane A:0.5 mg U937 Whole Cell Lysate. 4 uLCCDC5 Antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: CCDC5 Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :32 kDa](http://images.novusbio.com/fullsize/CCDC5-Antibody-Western-Blot-NBP3-06419-img0003.jpg "Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 Antibody was immunoprecipitated using: Lane A:0.5 mg U937 Whole Cell Lysate. 4 uLCCDC5 Antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: CCDC5 Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :32 kDa")
![Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 antibody at 1:500 dilution. Lane A: HL60 Whole Cell Lysate Lane B: U937 Whole Cell Lysate Lane C: MOLT4 Whole Cell Lysate Lane D: Daudi Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:32 kDa](http://images.novusbio.com/fullsize/CCDC5-Antibody-Western-Blot-NBP3-06419-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CCDC5 Antibody [NBP3-06419] - Immunofluorescence staining of CCDC5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06419 antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06419_rabbit-polyclonal-ccdc5-antibody-23520252135125.png)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP2-98881-img0005.jpg "Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa")
![Immunoprecipitation: p16INK4a/CDKN2A Antibody [NBP2-98881] - p16INK4a/CDKN2A was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-p16INK4a/CDKN2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-p16INK4a/CDKN2A rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Immunoprecipitation-NBP2-98881-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody [NBP2-98881] - Immunofluorescence staining of p16INK4a/CDKN2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98881 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98881_rabbit-polyclonal-p16ink4a-cdkn2a-antibody-235202512205526.png)
![Immunohistochemistry-Paraffin: RAD9B Antibody - Azide and BSA Free [NBP3-26072] - Immunochemical staining of human RAD9B in human kidney with NBP3-26072 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-26072_rabbit-rad9b-pab-azide-and-bsa-free-136202422345047.png "Immunohistochemistry-Paraffin: RAD9B Antibody - Azide and BSA Free [NBP3-26072] - Immunochemical staining of human RAD9B in human kidney with NBP3-26072 at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: RAD9B Antibody - Azide and BSA Free [NBP3-26072] - NBP3-26072 at 1:500 dilution. Lane A: THP-1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa](http://images.novusbio.com/fullsize/nbp3-26072_rabbit-rad9b-pab-azide-and-bsa-free-136202422345068.png)
![Immunohistochemistry-Paraffin: RAD9B Antibody - Azide and BSA Free [NBP3-26072] - Immunochemical staining of human RAD9B in human skeletal muscle with NBP3-26072 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-26072_rabbit-rad9b-pab-azide-and-bsa-free-1362024223417.png)
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/TTK-Antibody-Immunohistochemistry-Paraffin-NBP2-98832-img0002.jpg "Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).")
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human RPS27 in human lung cancer with NBP2-98832 (1:1000).](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-235202511444413.png)
![Immunocytochemistry/Immunofluorescence: TTK Antibody [NBP2-98832] - Immunofluorescence staining of TTK in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98832 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-23520252135167.png)
![Immunohistochemistry: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32690) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32690_rabbit-p18ink4c-cdkn2c-mab-je36-09-298202414115420.jpg "Immunohistochemistry: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32690) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32690 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32690 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32690_-western-blot-639204597145889295.jpg)
![Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Western-Blot-NBP1-02798-img0003.jpg "Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.")
![Immunohistochemistry-Paraffin: MAD2L2 Antibody [NBP1-02798] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Immunohistochemistry-Paraffin-NBP1-02798-img0001.jpg)
![Western Blot: BTBD1 Antibody [NBP2-87087] - Host: Rabbit. Target Name: BTBD1. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/BTBD1-Antibody-Western-Blot-NBP2-87087-img0003.jpg "Western Blot: BTBD1 Antibody [NBP2-87087] - Host: Rabbit. Target Name: BTBD1. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml")
![Western Blot: BTBD1 Antibody [NBP2-87087] - WB Suggested Anti-BTBD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysateBTBD1 is supported by BioGPS gene expression data to be expressed in 721_B](http://images.novusbio.com/fullsize/BTBD1-Antibody-Western-Blot-NBP2-87087-img0001.jpg)
![Western Blot: BTBD1 Antibody [NBP2-87087] - Host: Rabbit. Target Name: BTBD1. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/BTBD1-Antibody-Western-Blot-NBP2-87087-img0002.jpg)
![Western Blot: BTBD1 Antibody [NBP2-87087] - Host: Rabbit. Target Name: BTBD1. Sample Type: Human Adult Placenta. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/BTBD1-Antibody-Western-Blot-NBP2-87087-img0004.jpg)