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![Flow Cytometry: N-Cadherin Antibody (13A9) [Alexa Fluor® 488] [NBP1-48309AF488] - An intracellular stain was performed on A549 cells with N-Cadherin Antibody (13A9) NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Alexa-Fluor-R-488-Flow-Cytometry-NBP1-48309AF488-img0002.jpg "Flow Cytometry: N-Cadherin Antibody (13A9) [Alexa Fluor® 488] [NBP1-48309AF488] - An intracellular stain was performed on A549 cells with N-Cadherin Antibody (13A9) NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: N-Cadherin Antibody (13A9) [Alexa Fluor 488] [NBP1-48309AF488] - An intracellular stain was performed on HeLa cells with N-Cadherin Antibody (13A9)NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Alexa-Fluor-488-Flow-Cytometry-NBP1-48309AF488-img0001.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85730] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding SEPT7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85730-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85730] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding SEPT7 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85730] - Staining of human cerebral cortex, lymph node, skeletal muscle and testis using Anti-SEPT7 antibody NBP1-85730 (A) shows similar protein distribution across tissues to independent antibody NBP1-85731 (B).](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85730-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-7 Antibody [NBP1-85730] - Staining of human cell line U-2 OS shows localization to actin filaments & midbody. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85730-img0017.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85730] - Staining of human cerebral cortex shows positivity in neuropil.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85730-img0011.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85730] - Staining of human lymph node shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85730-img0012.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85730] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85730-img0013.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85730] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85730-img0014.jpg)
