| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05384-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92651-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-19115-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99593-50ul | |||
![Immunohistochemistry;NPM1 Antibody[NBP3-29483]Detection of human and mouse NPM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma and mouse renal cell carcinoma . Antibody: Affinity purified rabbit anti-NPM1 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29484_rabbit-npm1-pab-78202410153513.jpg "Immunohistochemistry;NPM1 Antibody[NBP3-29483]Detection of human and mouse NPM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma and mouse renal cell carcinoma . Antibody: Affinity purified rabbit anti-NPM1 used at a dilution of 1:1,000 . Detection: DAB")
![Immunoprecipitation:NPM1 AntibodyNBP3-29484] -Detection of human NPM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody NBP3-29484 used for IP at 10 µg/mg lysate. NPM1 was also immunoprecipitated by rabbit anti-NPM1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated NPM1, was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29484_rabbit-npm1-pab-301220241941541.jpg)
![Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NEK1 antibody [HL2489] (NBP3-48690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48690_rabbit-nek1-mab-hl2489-western-blot-1411202518425217.jpg "Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NEK1 antibody [HL2489] (NBP3-48690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NEK1 antibody [HL2489] (NBP3-48690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48690_rabbit-nek1-mab-hl2489-western-blot-141120251824332.jpg)
![Immunohistochemistry:[NBP3-29335]-Detection of human NPM1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPM1.](http://images.novusbio.com/fullsize/nbp3-29335_rabbit-npm1-pab-23120251252823.jpg "Immunohistochemistry:[NBP3-29335]-Detection of human NPM1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPM1.")
![Western Blot: ILF3 Antibody [NBP1-58225] - Titration: 0.0625ug/ml Positive Control: Raji cell lysate.](http://images.novusbio.com/fullsize/ILF3-Antibody-Western-Blot-NBP1-58225-img0004.jpg "Western Blot: ILF3 Antibody [NBP1-58225] - Titration: 0.0625ug/ml Positive Control: Raji cell lysate.")
![Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-58225] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/ILF3-Antibody-Immunohistochemistry-Paraffin-NBP1-58225-img0005.jpg)
![Western Blot: RRM1 Antibody (4P4U1) [NBP3-16364] - Western blot analysis of extracts of various cell lines, using RRM1 Rabbit mAb (NBP3-16364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/RRM1-Antibody-4P4U1-Western-Blot-NBP3-16364-img0001.jpg "Western Blot: RRM1 Antibody (4P4U1) [NBP3-16364] - Western blot analysis of extracts of various cell lines, using RRM1 Rabbit mAb (NBP3-16364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: RRM1 Antibody (4P4U1) [NBP3-16364] - Western blot analysis of extracts of Rat lung, using RRM1 Rabbit mAb (NBP3-16364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/RRM1-Antibody-4P4U1-Western-Blot-NBP3-16364-img0002.jpg)
![Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MDM2-HDM2-[p-Ser166]-Antibody-Western-Blot-NBP3-05384-img0002.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 [p Ser166] Antibody - BSA Free [MDM2/HDM2] - Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using MDM2/HDM2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-05384_rabbit-polyclonal-mdm2-hdm2-p-ser166-antibody-5220251640233.jpg)
![Western Blot: Cullin 2 Antibody [NBP2-92651] - Analysis of extracts of various cell lines, using Cullin 2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Cullin-2-Antibody-Western-Blot-NBP2-92651-img0002.jpg "Western Blot: Cullin 2 Antibody [NBP2-92651] - Analysis of extracts of various cell lines, using Cullin 2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Cullin 2 Antibody [NBP2-92651] - Analysis of A-549 cells using Cullin 2 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cullin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92651-img0001.jpg)
![Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Western blot analysis of extracts of HepG2 cells, using CDCA5 Rabbit mAb (NBP3-16318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CDCA5-Antibody-1Y1C9-Western-Blot-NBP3-16318-img0002.jpg "Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Western blot analysis of extracts of HepG2 cells, using CDCA5 Rabbit mAb (NBP3-16318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Immunohistochemistry of paraffin-embedded human thyroid cancer using CDCA5 Rabbit mAb (NBP3-16318) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDCA5-Antibody-1Y1C9-Immunohistochemistry-Paraffin-NBP3-16318-img0001.jpg)
![Western Blot: DUSP6a Antibody [NB120-19115] - E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:1000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-DUSP6 )](http://images.novusbio.com/fullsize/DUSP6a-Antibody-Western-Blot-NB120-19115-img0001.jpg "Western Blot: DUSP6a Antibody [NB120-19115] - E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:1000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-DUSP6 )")
![Western Blot: DP2 Antibody [NBP2-88806] - WB Suggested Anti-TFDP2 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysateTFDP2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells](http://images.novusbio.com/fullsize/DP2-Antibody-Western-Blot-NBP2-88806-img0002.jpg "Western Blot: DP2 Antibody [NBP2-88806] - WB Suggested Anti-TFDP2 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysateTFDP2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells")
![Immunohistochemistry: DP2 Antibody [NBP2-88806] - Human kidney](http://images.novusbio.com/fullsize/DP2-Antibody-Immunohistochemistry-NBP2-88806-img0001.jpg)
![Western Blot: IFRD1 Antibody [NBP2-99593] - Anti-IFRD1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-99593-img0003.jpg "Western Blot: IFRD1 Antibody [NBP2-99593] - Anti-IFRD1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa")
![Immunocytochemistry/Immunofluorescence: IFRD1 Antibody [NBP2-99593] - Immunofluorescence staining of IFRD1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99593 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .](http://images.novusbio.com/fullsize/nbp2-99593_rabbit-polyclonal-ifrd1-antibody-235202521525033.png)